THE ANTICANCER EFFECT AND ANTI－DNA TOPOISOMERASE II EFFECT OF EXTRACTS OF CAMELLIA PTILOPHYLLA CHANG AND CAMELLIA SINENSIS

ＴＨＥＡＮＴＩＣＡＮＣＥＲＥＦＦＥＣＴＡＮＤＡＮＴＩ－ＤＮＡＴＯＰＯＩＳＯＭＥＲＡＳＥＩＩＥＦＦＥＣＴＯＦＥＸＴＲＡＣＴＳＯＦＣＡＭＥＬＬＩＡＰＴＩＬＯＰＨＹＬＬＡＣＨＡＮＧＡＮＤＣＡＭＥＬＬＩＡＳＩＮＥＮＳＩＳＸｉｅＢｉｎｓｆｅｎ；谢冰芬；ＬｉｕＺ...     

ISOLATION AND CHARACTERIZATION OF AN ADRIAMYCIN－RESISTANT SUBLINE OF THE HUMAN GASTRIC ADENOCARCINOMA CELLLINE

ＩＳＯＬＡＴＩＯＮＡＮＤＣＨＡＲＡＣＴＥＲＩＺＡＴＩＯＮＯＦＡＮＡＤＲＩＡＭＹＣＩＮ－ＲＥＳＩＳＴＡＮＴＳＵＢＬＩＮＥＯＦＴＨＥＨＵＭＡＮＧＡＳＴＲＩＣＡＤＥＮＯＣＡＲＣＩＮＯＭＡＣＥＬＬＬＩＮＥＷａｎｇＹａｎｐｉｎｇ；王艳萍；ＸｕＧａｎｇ；徐刚（Ｉ...     

COMPARATIVE PATHOLOGICAL AND FCM STUDIES ON THE PRIMARY AND METASTATIC CANCERS IN STOMACH AND BREAST

ＣＯＭＰＡＲＡＴＩＶＥＰＡＴＨＯＬＯＧＩＣＡＬＡＮＤＦＣＭＳＴＵＤＩＥＳＯＮＴＨＥＰＲＩＭＡＲＹＡＮＤＭＥＴＡＳＴＡＴＩＣＣＡＮＣＥＲＳＩＮＳＴＯＭＡＣＨＡＮＤＢＲＥＡＳＴ￥ＺｈａｎｇＸｉａｎｇｈｏｎｇ；张祥宏；ＺｈａｎｇＪｉｅｙｉｎｇ；张杰英；Ｘｉ...     

THE CERVIX MULTI－VIRUSES INFECTION AND THE DEVELOPMENT OF CERVICAL CARCINOMAS

ＴＨＥＣＥＲＶＩＸＭＵＬＴＩ－ＶＩＲＵＳＥＳＩＮＦＥＣＴＩＯＮＡＮＤＴＨＥＤＥＶＥＬＯＰＭＥＮＴＯＦＣＥＲＶＩＣＡＬＣＡＲＣＩＮＯＭＡＳＺｈａｎｇＷｅｉ张伟；ＪｉｎＳｎｕｎｑｉａｎ金顺钱；ＬｉｕＢｏｑｉ刘伯齐；ＬｉａｎｓＸｉａｏ梁肖；ＭｉｎｇＬｉｈｕ...     

THE EXPRESSION OF C－MYC AND N－RAS ONCOGENES IN HUMAN HEPATOCELLULAR CARCINOMA－AN IN SITU HYBRIDIZATION STUDY ON PARAFFIN EMBE

ＴＨＥＥＸＰＲＥＳＳＩＯＮＯＦＣ－ＭＹＣＡＮＤＮ－ＲＡＳＯＮＣＯＧＥＮＥＳＩＮＨＵＭＡＮＨＥＰＡＴＯＣＥＬＬＵＬＡＲＣＡＲＣＩＮＯＭＡ－ＡＮＩＮＳＩＴＵＨＹＢＲＩＤＩＺＡＴＩＯＮＳＴＵＤＹＯＮＰＡＲＡＦＦＩＮＥＭＢＥＤＤＥＤＴＩＳＳＵＥＳＥＣＴＩＯＮ...     

EXPERIMENTAL AND CLINICAL STUDY OF CONCOMITANT RADIATION THERAPY AND CHEMOTHERAPY FORCERVICAL CARCINOMA

ＥＸＰＥＲＩＭＥＮＴＡＬＡＮＤＣＬＩＮＩＣＡＬＳＴＵＤＹＯＦＣＯＮＣＯＭＩＴＡＮＴＲＡＤＩＡＴＩＯＮＴＨＥＲＡＰＹＡＮＤＣＨＥＭＯＴＨＥＲＡＰＹＦＯＲＣＥＲＶＩＣＡＬＣＡＲＣＩＮＯＭＡ￥ＷｅｉＫｅ；魏克；ＣａｉＳｈｕｍｏ；蔡树模；ＷａｎｇＸｉａｎｇ－...     

PROLIFERATION OF ANTI－CD_3 McAb AND IL－2 INDUCED SPLENOCYTES AND ANTITUMOR EFFECT OF THEIR CULTURE SUPERNATANTS

ＰＲＯＬＩＦＥＲＡＴＩＯＮＯＦＡＮＴＩ－ＣＤ_３ＭｃＡｂＡＮＤＩＬ－２ＩＮＤＵＣＥＤＳＰＬＥＮＯＣＹＴＥＳＡＮＤＡＮＴＩＴＵＭＯＲＥＦＦＥＣＴＯＦＴＨＥＩＲＣＵＬＴＵＲＥＳＵＰＥＲＮＡＴＡＮＴＳ￥ＳｈｅｎＧｕａｎｘｉｎ；沈关心；ＷａｎｇＸｉａｌｉｎ；?..     

THE BLOCKING EFFECTS OF GLYCYRRHIZE URALENSIS AND CHELIDONIUM MAJUS ON MUTAGENESIS INDUCED BY AFLATOXIN B1

ＴＨＥＢＬＯＣＫＩＮＧＥＦＦＥＣＴＳＯＦＧＬＹＣＹＲＲＨＩＺＥＵＲＡＬＥＮＳＩＳＡＮＤＣＨＥＬＩＤＯＮＩＵＭＭＡＪＵＳＯＮＭＵＴＡＧＥＮＥＳＩＳＩＮＤＵＣＥＤＢＹＡＦＬＡＴＯＸＩＮＢ１ＳｈｉＧｕｉｚｎｉ；史桂芝；ＪｉＸｉｎｈｕａ；纪新华；ＬｉａｎｇＹ...     

THE SIGNIFICANCE OF P53 GENE MUTATIONS AND EXPRESSIONS IN HUMAN COLORECTAL TUMORS

ＴＨＥＳＩＧＮＩＦＩＣＡＮＣＥＯＦＰ５３ＧＥＮＥＭＵＴＡＴＩＯＮＳＡＮＤＥＸＰＲＥＳＳＩＯＮＳＩＮＨＵＭＡＮＣＯＬＯＲＥＣＴＡＬＴＵＭＯＲＳＱｉａｎＨｕａ钱桦；ＹｕＢａｏｍｉｎｇ郁宝铭；ＺｈｏｕＸｉｇｅｎｇ周锡庚；ＷａｎｇＲｕｉｎｉａｎ王瑞年；Ｈｕａ...     

THE EFFECT OF ACTIVE COMPONENTS OF LYCIUM BARBARUM AND GARLIC（LB－GO）ON THE SYNTHESIS OF DNA AND ULTRASTRUCTURE OF U_（14） CERV

ＴＨＥＥＦＦＥＣＴＯＦＡＣＴＩＶＥＣＯＭＰＯＮＥＮＴＳＯＦＬＹＣＩＵＭＢＡＲＢＡＲＵＭＡＮＤＧＡＲＬＩＣ（ＬＢ－ＧＯ）ＯＮＴＨＥＳＹＮＴＨＥＳＩＳＯＦＤＮＡＡＮＤＵＬＴＲＡＳＴＲＵＣＴＵＲＥＯＦＵ_（１４）ＣＥＲＶＩＸＣＡＮＣＥＲＣＥＬＬＳＩＮＭＩＣ?..     

The expression of Cathepsin-D,C-erbB-2 and EGFR in breast cancer and its correlation to lymphatic metastasis

The expression of Cathepsin-D (Cath-D), c-erbB-2 and EGFR in breast cancer and its correlation to lymphatic metastases were studied in 277 cases by immunohistochemical technique. Positive staining of Cath-D was detected in 107 cases (38.62%). Among those, 89 cases (83.17%)had documented metastases in the lymph nodes. One hundred and seventy cases (61.38%) stained negative for Cath-D. Of which 64 cases (37.64%)had detectable lymphatic metastases. There is a significant difference in the rate of the lymphatic metastases between the Cath-D positive and Cath-D negative groups (x ²=55.05P<0.0001). Fifty-six out of 107 Cath-D positive cases (52.23%) were c -erbB - 2 positive as well. However, only 27 out of 170 Cath - D negative cases (15.88%) were c-erbB-2 positive. The positive rate of c-erbB - 2 in Cath - D positive group was significantly different from that of Cath - D negative group (x ²=41.58P<0.0001). Among those 107 Cath - D positive cases,49 cases (45.79%) were EGFR positive. Only 24 cases (14.12%) were EGFR positive among the 170 Cath - D negative cases. The positive rate of EGFR between these two groups was also significantly different (x ²=33.95P<0.0001). An analysis of the three mentioned markers, the lymph node metastasis and tumor size suggests that Cath - D is the most valuable indicator for tumor aggressiveness. Breast cancer cases with a positive Cath - D staining are more likely to have lymphatic metastases and a poor prognosis. Therefore, alternative therapeutic strategies and close follow ups are appropriate for these patients.     

乳腺癌组织中Cath-D、c-erbB-2的表达及其与肿瘤标志物水平、淋巴结转移的相关性

目的观察乳腺癌患者病理组织中Cath-D、c-erbB-2的表达情况,并分析其与淋巴结转移、肿瘤标志物水平的相关性。方法选取接受手术治疗的乳腺癌患者为研究对象,同时选取同期接受手术治疗的乳腺纤维腺瘤患者为对照。观察乳腺癌(有、无淋巴转移组)、乳腺纤维腺瘤患者组织切片Cath-D、c-erbB-2阳性表达情况,比较乳腺癌(有、无淋巴转移组)、乳腺纤维腺瘤患者血清肿瘤标志物水平的差异,分析淋巴结转移的乳腺癌患者Cath-D、c-erbB-2阳性表达与肿瘤标志物水平的相关性。结果乳腺癌组患者的组织切片Cath-D、c-erbB-2阳性表达率均高于乳腺纤维腺瘤组(χ²=51.796、70.090,P均<0.001);有淋巴结转移的乳腺癌患者组织切片Cath-D、c-erbB-2阳性表达率高于无淋巴结转移组。乳腺癌组患者血清CEA、CA15-3、CA125水平高于乳腺纤维腺瘤组(t=52.270、58.784、76.349,P<0.001);乳腺癌组有淋巴结转移组患者血清CEA、CA15-3、CA125水平高于无淋巴结转移组(t=16.681、27.880、23.216,P<0.001)。有淋巴结转移的乳腺癌患者Cath-D、c-erbB-2阳性表达率与血清CEA、CA15-3、CA125水平正相关。结论乳腺癌患者病理组织中Cath-D、c-erbB-2阳性表达率较高,且与淋巴结转移和肿瘤标志物水平相关。     

组蛋白酶D和c-erbB-2在乳腺癌中的表达及其与预后的关系

目的探讨乳腺癌组织中c-erbB-2和组织蛋白酶D（cath—D）的表达水平与临床病理及预后的关系。方法选取128例乳腺癌患者,采用免疫组化法检测c—erbB-2和cath—D蛋白的表达水平。并与患者的临床病理指标和预后做关联分析。结果c-erbB-2阳性率为36．5％,cath-D阳性表达率为40．9％。cath—D的表达水平与患者肿块直径大小显著相关,肿瘤直径〉5cm者cath—D的阳性率显著高于〈2cm者（x^2=21．0,P〈0．0001）;cath-D阴性的68例中,24例（35．29％）有淋巴结转移,cath-D阳性者淋巴结转移率（63．83％）明显高于cath-D阴性者（P〈O．0001）。c—erbB-2表达比不表达患者预后差（P=0．01）。结论c-erbB-2阳性表达和cath-D阳性表达者,恶性程度高,易转移、预后差。     

c－erbB－2和EGFR在胃癌中表达的研究

应用ＡＢＣ和ＬＳＡＢ法对７５例胃癌及癌旁组织进行了ｃ－ｅｒｂＢ－２和ＥＧＦＲ表达的研究。结果：１）７５例胃癌ｃ－ｅｒｂＢ－２表达阳性率为１８．７％，阳性表达只限于癌灶，而癌旁组织均为阴性。ｃｅｒｂＢ－２在高分化胃癌、大体局限型胃癌中的阳性率较高（Ｐ＜０．０５）。２）ＥＧＦＲ表达阳性率为６１．３％，癌旁组织、新生血管有阳性表达。ＥＧＦＲ表达与胃癌的大体类型、生长方式、分化程度、淋巴结受累和远处转移至正相关（Ｐ＜０．０５）。３）ｃ－ｅｒｂＢ－２表达与ＥＧＦＲ表达无明显关系。结果说明：ｃ－ｅｒｂＢ－２表达出现于胃粘膜癌变的后期，是恶性细胞的标志；ＥＧＦＲ表达与胃癌的恶性程度有明显关系；ｃ－ｅｒｂＢ－２和ＥＧＦＲ在胃癌中的表达是互相独立的。     

EXPRESSION OF ONCOGENE AND ANTI-ONCOGENE IN MALE BREAST CARCINOMA

ＥＸＰＲＥＳＳＩＯＮＯＦＯＮＣＯＧＥＮＥＡＮＤＡＮＴＩ－ＯＮＣＯＧＥＮＥＩＮＭＡＬＥＢＲＥＡＳＴＣＡＲＣＩＮＯＭＡ￥ＸｕＬｉａｎｇｚｈｏｎｇ；许良中；ｚｈａｎｇＴｉｎｇｑｉｕ；张廷璆ＨｕＸｉａｏｊｕａｎ；胡筱娟；ＺｈａｎｇＪｉｅ；张捷（Ｄｅｐａｒｔｍ...     

男性乳腺癌癌基因与抗癌基因表达的初步研究

本文观察了１２例男性乳腺癌的癌基因与抗癌基因产物的表达。１２例中ｃ－ｍｙｃ阳性６例，ＥＧＦＲ阳性６例，ｃ－ｅｒｂＢ－２阳性４例，Ｎ－ｒａｓ阳性２例，Ｒｂ阳性５例，ｐ５３阳性３例。上述癌基因与抗癌基因产物中，全部阳性的１例，全部阴性的４例。此外还作了ＣａｔｈｅｐｓｉｎＤ、ＥＲ、ＰＲ、ＡＲ、ＰＣＮＡ与ＡｇＮＯＲ检测，ｃ－ｅｒｂＢ－２或ｐ５３阳性的病例，ＣａｔｈｅｐｓｉｎＤ均阳性。     

Study of c-erbB-2 protein and epidermal growth factor receptor expression and DNA ploidy pattern in colorectal carcinoma

Correlation of c-erbB-2 protein (n = 44), epidermal growth factor receptor (EGFR) (n = 41) expression, and DNA ploidy pattern (n = 42) with clinical outcomes of human colorectal cancers was studied. Using monoclonal antibodies against c-erbB-2 protein and EGFR, an immunohis-tochemical study of the expression of c-erbB-2 protein and EGFR in frozen tissue sections from the lesion was performed. There was no significant correlation between the expression of c-erbB-2 protein and clinicopatho-logical findings such as, tumor size, histological type, depth of invasion, lymph node metastasis, lymphatic vessel invasion, or venous invasion. However, the incidence of c-erbB-2 protein expression in Dukes D was significantly higher (9/10, 90%) than that in Dukes A to C (16/34, 47.1%). Similar tendency was also observed in the expression of EGFR. Aneuploid case was observed in 12 of observed 25 (48%) cases without lymph node metastasis, while it was observed in 16 of 17 cases (94.1%) with lymph node metastasis and there was significant association between DNA ploidy pattern and lymph node metastasis (P< 0.01) and most of the cases (17/20, 85%) were aneuploidy in Dukes C and D. The results above suggest that the expression of c-erbB-2 protein or EGFR was associated with distant metastasis, while on the other hand DNA ploidy pattern was correlated with lymph node metastasis. © 1993 Wiley-Liss, Inc.     

大肠腺癌EGFR和ras p21表达与淋巴结转移的关系

目的 探索大肠腺癌ＥＧＦＲ和ｒａｓ ｐ２１表达与淋巴结转移的关系。方法 采用免疫组织化学ＬＳＡＢ法,对７０例大肠腺癌连续切片组织同步进行ＥＧＦＲ和ｒａｓ ｐ２１检测。结果 在有淋巴结转移的３５例大肠腺癌中,ＥＧＦＲ、ｒａｓ ｐ２１及ＥＧＦＲ和ｒａｓ ｐ２１共同呈阳性表达者分别是２４例（６８．６％）、２８例（８０．０％））和２２例（６２．９％）,在无淋巴结转移的３５例大肠腺癌中,ＥＧＦＲ、ｒａｓ ｐ     

腋淋巴结阴性乳腺癌中组织蛋白酶D表达意义的比较研究

目的 对组织蛋白酶D(Cath-D)在腋淋巴结阴性(ANN)乳腺癌中不同部位的表达与预后的相关性进行比较性研究。方法 根据术后病理检查腋淋巴结有否转移及根据随访结果选取192例乳腺癌病例作为本项目研究对象,并将其分为ANN预后差组、ANN预后好组和淋巴结阳性(ANP)组3组。采用免疫组化S-P法检测全部病例中Cath-D的表达,根据不同的表达部位,采用三种不同的评价方法分析比较,并用χ2。检验、χ2分割法和u检验进行统计学处理。结果 Cath-D阳性表达为棕黄色着色,定位于部分癌细胞和间质细胞胞质中;ANN预后差组和ANP组癌细胞Cath-D阳性率明显高于预后好组(χ2=23.20,P<0.01);ANP组表达与预后差组间无统计学差异(χ2=0.19,P>0.05);三组间间质细胞Cath-D表达阳性率无统计学差异(χ2=1.56,P>0.05);癌细胞和间质细胞表达均计入阳性率时,其阳性率与仅观察癌细胞表达无显著意义的提高(u1=0.47;u2=1.41;P>0.05)。结论 Cath-D可以作为ANN乳腺癌预后差的标志物之一,仅观察评价其在癌细胞中的表达是比较可靠、实用和简便的方法。     

MMP-9、Cath-D阳性与胃癌生物学行为相关性

目的:探讨MMP-9和Cath-D阳性与胃癌生物学行为的关系。方法:采用免疫组化S-P法,检测MMP-9和Cath-D在128例原发性胃癌的阳性率。结果:128例胃癌组织中MMP-9和Cath-D阳性表达与胃癌生长方式、浸润深度、TNM分期、淋巴结转移密切相关(P<0.05);弥漫型胃癌MMP-9阳性率明显高于肠型胃癌(P<0.01);MMP-9和Cath-D阳性的胃癌患者五年生存率较阴性者低(P<0.05);两者在胃癌中阳性呈正相关,MMP-9(-)/Cath-D(-)组发生淋巴结转移和浆膜浸润的比率均明显低于MMP-9( )/Cath-D( )组、MMP-9( )/cath-D(-)组和MMP-9(-)/Cath-D( )组(P<0.05)。结论:MMP-9和(Sath-D与胃癌的生长、浸润、转移及预后均有密切关系,同时进行MMP-9和Cath-D免疫组化检测对于评估胃癌的预后有意义。