两种方法保存同种异体髌腱移植重建膝关节交叉韧带的光镜电镜观察

目的从实验角度观察采用程序冷冻液氮保存和-80℃深低温保存同种异体髌腱及移植重建膝关节交叉韧带后的组织学变化,为临床同种异体腱移植提供理论依据。方法分别将兔的1/2骨-髌腱-骨复合体经程序冷冻液氮保存2周和-80℃深低温保存2周后,观察冻存变化差异并行同种异体移植重建前交叉韧带,分别于术后3周和8周在光镜和电镜下观察其组织学变化。结果光镜和电镜下可见,经程序冷冻液氮保存方法处理后,冷冻损伤较-80℃深低温保存方法轻微; -80℃深低温保存组和程序冷冻液氮保存组的移植物在术后愈合过程中的组织学行为均与自体移植组相似,而程序冷冻液氮保存组更接近于自体移植组。结论经程序冷冻液氮保存和-80℃深低温保存方法冻存的兔异体髌腱重建膝关节交叉韧带后愈合过程和自体韧带移植重建过程相似,但程序冷冻液氮保存法优于传统的-80℃深低温保存法。     

液氮冷冻术配合rhEGF治疗角膜炎的临床观察

目的探讨液氮冷冻术配合重组人表皮生长因子衍生物滴眼液治疗单纯疱疹病毒性角膜炎（溃疡型）的疗效观察。方法对30眼单纯疱疹病毒性角膜炎（溃疡型）施行液氮冷冻术配合rhEGF治疗，31眼单纯疱疹病毒性角膜炎（溃疡型）单纯施行液氮冷冻术治疗。术后对角膜上皮的愈合速度与透明度进行比较。结果实验组30眼，所有治疗病例临床症状好转，视力不同程度恢复，角膜透明度增加，角膜混浊面积缩小，病情稳定，无复发，有效率均达100％，对角摸组织无永久性损伤。结论液氮冷冻术配合rhEGF治疗单纯疱疹病毒性角膜炎（溃疡型）有促进角膜上皮损伤修复的作用，临床疗效好，病程明显缩短，是一种简单、安全、理想的治疗方法。     

液氮冷冻与激光治疗雀斑的效果比较

目的对比研究液氮冷冻与激光治疗雀斑的效果。方法选取2016年12月至2017年4月兰考县第三人民医院收治的76例雀斑患者,依照治疗方法分为液氮冷冻组(38例)与激光组(38例)。激光组接受激光治疗,液氮冷冻组接受液氮冷冻治疗。观察两组治疗效果、不良反应发生率和复发率。结果治疗8周后,液氮冷冻组治愈率(92.11%)高于激光组(47.37%)(P<0.05);液氮冷冻组术后红斑发生率低于激光组(P<0.05);随访6～12个月液氮冷冻组复发率低于激光组(P<0.05)。结论与激光治疗对比,液氮冷冻治疗雀斑治愈率高,可显著降低色素沉着等不良反应发生率,减少复发。     

液氮冷冻与复方倍他米松局部注射治疗局限型斑秃的效果比较

目的比较液氮浅低温冷冻法与复方倍他米松注射液局部注射法治疗局限型斑秃的治疗效果。方法将112例局限型斑秃患者随机分为冷冻组60例和倍他米松组52例,两组分别用液氮或倍他米松局部治疗,并口服复方甘草酸苷片和胱氨酸片。结果两组患者疗效比较差异无统计学意义（P〉0.05）。结论液氮浅低温冷冻法和复方倍他米松注射液局部注射法治疗局限型斑秃效果显著,疗效相当。     

局部曲安缩松封闭加液氮冷冻治疗瘢痕疙瘩

目的 :观察局部曲安缩松封闭加液氮冷冻治疗瘢痕疙瘩的临床疗效。方法 :采用随机、单盲、对照法将 86例病人分为治疗组 46例、对照组 40例。治疗组 46例局部曲安缩松封闭加液氮冷冻治疗瘢痕疙瘩 ,对照组 40例单纯用液氮冷冻治疗。结果 :治疗组与对照组治愈率有非常显著差异性P <0 0 0 1,治疗组临床疗效优于对照组。结论 :局部曲安缩松封闭加液氮冷冻是治疗瘢痕疙瘩的较有效的方法。     

液氮冷冻联合白癜酊治疗局限型白癜风107例疗效观察

目的比较单一液氮冷冻与液氮冷冻联合白癜酊治疗局限型白癜风的疗效。方法将180例局限型白癜风患者随机分为治疗组与对照组,治疗组接受液氮冷冻联合白癜酊治疗,对照组只接受冷冻治疗。结果经过2个月的规律治疗后,治疗组总有效率明显高于对照组(P<0.05)。结论液氮冷冻联合白癜酊治疗局限型白癜风较单一液氮冷冻治疗疗效显著。     

小鼠窦前卵泡的玻璃化冷冻方法研究

目的对性成熟前小鼠的窦前卵泡采用不同的方法进行玻璃化冷冻,探讨不同冷冻方法对卵泡复苏率的影响。方法用Ⅰ型胶原酶和脱氧核糖核酸酶Ⅰ分离10-14dICR雌鼠的卵巢,共进行A、B两个实验,每次实验对获得的卵泡分为4组,1组作为新鲜对照组,其余3组采用不同的冷冻方法处理,试验A采用常规玻璃化法、-205℃玻璃化法和液氮网篮法;试验B采用-205℃玻璃化法、液氮网篮法和-205℃表面玻璃化法,卵泡复苏后进行台盼蓝染色,计算各组卵泡复苏率。结果液氮网篮和-205℃玻璃化冷冻的冷冻效果一致,两种方法的卵泡复苏率差别无统计学意义;常规玻璃化的冷冻效果最差,-205℃表面玻璃化冷冻的冷冻效果最好,其冷冻小鼠窦前卵泡的复苏率达（83．02±4．10）％。结论-205℃表面玻璃化是一种较好的冷冻窦前卵泡的方法。     

自然冻融蟾蜍眼球的形态学观察

本实验采用自然和离体冻融（－２２），冷冻蟾蜍眼球。将其分为在体和离体冻融。冷冻６０分钟后，用０．２％甘油３０℃复温，１－２分钟后复苏，速将其眼球经组织学常规处理。ＨＥ染色，ｏｌｙｍｐｕｓ显微镜观察及图像分析冻融后蟾蜍眼球组织结构的变化。离体眼球组，显微镜下可见：角膜，视网膜，脉络膜，巩膜细胞体积增大，颗粒增多现象。纤维呈透明玻璃样变。在体冷冻复温组，眼球组织结构完整。各种细胞和纤维清楚可见，实验结     

冷冻联合光动力治疗尖锐湿疣疗效观察

目的探讨液氮冷冻联合光动力疗法治疗尖锐湿疣的疗效。方法治疗组200例采用液氮冷冻联合光动力治疗,对照组60例采用液氮冷冻联合鬼臼毒素治疗,比较两组疗效。结果治疗组痊愈率高于对照组（χ2=9.28,P〈0.05）,复发率低于对照组（χ2=11.05,P〈0.05）。结论液氮冷冻联合光动力疗法可有效治疗尖锐湿疣,此治愈率优于液氮冷冻联合鬼臼毒素治疗方法,且复发率低。     

液氮棉沾冷冻法治疗慢性宫颈炎937例临床分析

<正> 近年来国内开展了对慢性宫颈炎电灼、激光、液氮冷冻等疗法,取得了较好的治疗效果。其中液氮接触冷冻法及喷射冷冻法,虽然疗效满意,但萍在许多不足之处。接触冷冻法对宫颈外形不整者,因无法密切接触而影响疗效;喷射冷冻法如果掌握不当,易损伤阴道壁。为此,笔者在液氮冷冻法的基础上,进行了革新,创立了液氮柿沽冷冻法,并深入工厂对937例患者进行治疗,经随诊观察疗效满意,且具许多优点,现报道如下:     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.