张力吻合对动脉顺应性影响的实验研究

目的 探讨动脉张力吻合后其顺应性的变化。方法 在体测定犬股动脉非吻合、正常吻合及张力吻合后不同时相点吻合口段的压力与直径相应的数据，推算动脉非吻合、正常及张力吻合后的顺应性，确定动脉顺应性与平均血压的关系。结果 在各时相点，吻合组动脉的顺应性低于非吻合组，张力吻合组动脉的顺应性低于正常吻合组，术后14d降低最显著。结论 动脉张力吻合术后近期吻合口段的可扩张能力较正常吻合动脉及正常动脉有明显减弱，张力吻合对吻合口段动脉的功能有较大影响。     

动脉张力吻合后的顺应性变化

通过在体测定狗股动脉非吻合、正常吻合及张力吻合后不同时相点吻合口段的压力与直径相应的数据 ,计算出动脉非吻合、正常及张力吻合后的顺应性 ,顺应性与平均血压曲线用幂函数 C=bem Pa进行拟合 ,建立顺应性与平均血压的关系。结果表明 :在各时相点 ,吻合组的顺应性低于非吻合组 ,张力吻合组吻合口段动脉的顺应性低于正常吻合组 ,术后 14 d降低最显著。反映出动脉张力吻合术后近期吻合口段的可扩张能力较正常吻合动脉及正常动脉有明显减弱。张力吻合对吻合口段动脉的功能有较大影响     

狗股动脉吻接后顺应性变化的形态学基础

目的：探讨动脉吻接后形态学变化、结构成分含量与顺应性变化之间的关系。方法：取术后１天、３天、７天、１４天、３０天、７５天吻合口动脉段,切片分别用ＨＥ,Ｗｅｉｇｅｒｔ,Ａｎｉｌｉｎｅ ｂｌｕｅ,Ｎｅｕｂｅｒｔ法染色,光科像珞结构成分相对含量。结果：术后１４天,吻合口已基本疤痕化,各时相点弹性纤维含量变化无显著性差异,胶原纤维含量呈增加之趋势,Ｃ／Ｅ值术后逐日增高,第１４天最高,结论：血和过程,结构成     

动脉正常吻接和张力吻接后顺应性对比研究

通过在体测定狗股动脉正常吻接和张力吻接后不同时相点吻合口段的压力与直径相应的数据，从而得出动脉在正常及张力吻接后的顺应性，以及顺应性与平均血压的关系。结果表明在各时相点，张力吻接组吻合口段动脉的顺应性均低于正常吻接组。反映出动脉张力吻接后吻合口段的可扩张能力较正常吻接动脉有明显减弱，术后14天减弱最显著。张力吻合对吻合口段动脉的功能有较大影响。     

动脉正常吻接和张力吻接后顺应性对比研究

通过在体测定狗股动脉正常吻接和张力吻接后不同时相点吻合口段的压力与直接相应的数据,从而得出动脉在正常及张力吻接后的顺应性,以及顺应性与平均血压的关系。结果表明：在各时相点,张力吻接组吻合口段动脉的顺应性均低于正常吻接组,反映出动脉张力吻接合吻合口段的可扩张能力较正常吻接动脉有明显减弱,术后14天减弱最显著,张力吻合对吻合口段动脉的功能有较大影响。     

小动脉吻合后弹性纤维构筑及弹性蛋白原表达变化

目的：探讨小动脉吻合术后弹性纤维的构筑及弹性蛋白原mRNA表达的变化。方法：采用光镜、扫描电镜及寡核苷酸探针原位杂交方法，观察大鼠股动脉吻合术后3d、7d、14d、21d、30d和90d弹性纤维的构筑及弹性蛋白原mRNA表达。结果：吻合术后3d即有弹性蛋白原mRNA的表达，3～7d达到高峰，随后逐渐下降；术后14d吻合口区弹性纤维含量逐渐增多，术后30d增高明显，同时弹性纤维的形态结构趋于正常。结论：动脉吻合术后弹性纤维的重构分为：静止期、增生期、重建期。弹性蛋白原mRNA表达与弹性纤维的增生出现峰值的时间不同步。吻合后弹性纤维的重建修复为一种新生内膜型模式。     

入颅动脉弹性成分变化的形态学观察和顺应性的应用研究

本研究用组织学方法和图像分析系统研究了颈内动脉、大脑中动脉和推动脉、基底动脉两个系统中弹性纤维和胶原纤维含量的变化，从颅外至颅内两个系统中弹性纤维和胶原纤维含量均有下降。根据压力－容积变化关系，结果表明大脑中动脉的顺应性明显低于颈内动脉。本研究结果支持了高血压性脑出血与脑动脉壁薄弱有关的观点。     

弹性纤维在吻合后动脉修复中的作用

目的 研究动脉吻合术后弹性纤维在修复过程中的作用。方法 在手术显微镜下将90只大鼠股动脉横行切断后行端端吻合术，在手术后3、7、14、21、30、90d，采用Weigert弹性纤维染色及甲酸消化法扫描电镜观察弹性纤维在动脉吻合术后的修复过程。结果 动脉吻合术后弹性纤维的重构分为：静止期、增生期、重建期。结论 动脉壁的弹性纤维在动脉吻合术后经历了重建过程，吻合后弹性纤维的修复为新生内膜的模式。动脉吻合术后30d弹性纤维的含量明显增高及弹性纤维的形态结构趋于正常。     

胎儿静脉壁组织结构增龄性变化的定量分析

目的：探讨胎儿脐带静脉随胎龄变化的形态学改变，为临床挑选适当的血管代用品提供科学的理论依据。方法：33例新鲜脐带，常规石蜡包埋、切片、HE染组织结构，Weigert,Aniline Blue及桔黄G分染弹性纤维，胶原纤维和平滑肌，光镜观察及计算机图像分析。结果：随胎龄随长，脐静脉管径、中膜厚度、中膜弹性纤维含量逐渐增多；平滑肌含量及细胞核的数密度和面密度在37－40周最大（P＜0．05），胶原纤维则最小，因而此时段顺应性最好。结论：脐静脉管壁中各成分的含量随胎龄变化呈不等比增长，37－40周时血管顺应性最好。建议选用此时段胎龄的脐静脉作为移植材料。     

动脉吻接后的顺应性变化

本实验通过在体测定狗股动脉端端吻合前及吻合后不同时相点的压力与直径相应数据，推导出动脉吻接前后的顺应性，找出顺应性与平均血压的关系。结果表明；动脉吻接后吻合口段的顺应性较吻接前逐日下降，吻俣扣１４天下降最明显，以后有所回升。     

Biochemical analysis of collagen and elastin synthesis in the balloon injured rat carotid artery.

INTRODUCTION: Balloon catheter denudation of the endothelium in the common carotid artery of the rat is routinely used as a model of neointimal lesion development. The response to endothelial denuding injury involves proliferation and migration of medial smooth muscle cells (SMCs), with the formation of a dramatically thickened neointima. While many studies have focused on the kinetics of the early proliferative and migration responses, much less attention has been paid to the pronounced accumulation of extracellular matrix that occurs as the neointima grows. The purpose of this study was to measure collagen and elastin content, and to assess collagen and elastin synthesis in injured rat carotid arteries. METHODS: Male Sprague-Dawley rats were subject to balloon catheter injury of the left carotid artery using a 2F embolectomy catheter. Rats were sacrificed at different time points after injury, and both carotids left (injured) and right (uninjured and control) were used for measurement of elastin and collagen synthesis (7 days) and content (7, 21 and 60 days). RESULTS: Elastin and collagen syntheses were significantly increased in the injured carotids at 7 days after injury. The increase in elastin synthesis was more dramatic (100% compared to control) than the increase in collagen synthesis (50% compared to control). Both elastin and collagen content were significantly increased by 21 days, and contents were further increased by 60 days in the injured carotid compared to uninjured controls. At 60 days, collagen content of the injured vessels was 2.09+/-0.01 mg/arterial segment compared to 1.32+/-0.08 mg/arterial segment in controls (P=.01). Elastin content of injured vessels was 2.40+/-0.43 mg/arterial segment compared to 1.19+/-0.25 mg/arterial segment in controls (P=.03). CONCLUSIONS: Collagen and elastin contents were significantly increased following injury of the rat carotid. This study provides for the first time a biochemical assessment of collagen and elastin in the balloon-injured rat carotid artery.     

Hydrostatic pressure independently increases elastin and collagen co-expression in small-diameter engineered arterial constructs

Prior studies have demonstrated that smooth muscle cell (SMC) proliferation, migration, and extracellular matrix production increase with hydrostatic pressure in vitro. We have engineered highly compliant small-diameter arterial constructs by culturing primary adult baboon arterial SMCs under pulsatile perfusion on tubular, porous, elastomeric scaffolds composed of poly(glycerol sebacate) (PGS). This study investigates the effect of hydrostatic pressure on the biological and mechanical properties of PGS-based engineered arterial constructs. Pressure was raised using a downstream needle valve during perfusion while preserving flow rate and pulsatility, and constructs were evaluated by pressure-diameter testing and biochemical assays for collagen and elastin. Pressurized constructs contained half as much insoluble elastin as baboon common carotid arteries but were significantly less compliant, while constructs cultured at low hydrostatic pressure contained one-third as much insoluble elastin as baboon carotids and were similar in compliance. Hydrostatic pressure significantly increased construct burst pressure, collagen and insoluble elastin content, and soluble elastin concentration in culture medium. All arteries and constructs exhibited elastic recovery during pressure cycling. Hydrostatic pressure did not appear to affect radial distribution of SMCs, collagens I and III, and elastin. These results provide insights into the control of engineered smooth muscle tissue properties using hydrostatic pressure.