荧光定量PCR法在丙型肝炎病毒核糖核酸检测中的应用

目的：通过与荧光半定量PCR及传统逆转录PCR（RT-PCR）比较,探讨荧光定量PCR（FQ-PCR）在丙型肝炎病毒核糖核酸（HCV RNA）检测中的应用价值。方法：采用FQ-PCR、荧光半定量PCR及RT-PCR三种方法同步检测180例丙型肝炎患者HCV RNA含量,所得结果采用χ^2检验。结果：FQ-PCR在血浆中及单个核细胞中的阳性检出例数最多,分别占78.3%和83.7%。无论是在血浆中或单个核细胞测定,FQ-PCR阳性检出率均明显高于同步检测的荧光半定量PCR及RT-PCR（P〈0.01）。以RT-PCR为标准,当HCV RNA水平在10^3～5copies/ml时,FQ-PCR和荧光半定量PCR阳性检出水平相一致（P〉0.05）,明显高于RT-PCR（P〈0.01）。当HCV RNA水平在10^6～7cop-ies/ml时,3种检测方法的阳性检出率基本相同（P〉0.05）。结论：FQ-PCR检测HCV RNA具有高灵敏度、高特异性,且二次污染的可能性小。     

3种方法对发热伴血小板减少综合征检测结果比较

目的 采用实时荧光PCR法、酶联免疫吸附法及细胞培养分离病毒株的方法,对2013年临床疑似发热伴血小板减少综合征患者急性期血进行检测比较,寻求快速、准确的鉴定新布尼亚病毒感染的方法。方法 对180例临床疑似病例的急性期血液首先采用实时荧光PCR法检测,筛选出新布尼亚病毒核酸阳性的样本,同时将阳性样本采用酶联免疫吸附的方法检测抗体及采用VERO-E6细胞培养的方法分离病毒株。结果 180份样本经实时荧光PCR法检测,阳性率为42.78%(77/180 Ct值≤35曲线形态与阳性对照一致呈S型的),ELISA方法检测抗体阳性率为44.16%(34/77),将核酸检测阳性的77份样本全部感染VERO-E6细胞获得病毒株32株,阳性率为38.55%(32/77)。结论 实时荧光PCR法更适合于新布尼亚病毒感染者早期实验室快速诊断,发病在1w左右的病例不适合用ELISA的方法做确证实验。细胞培养分离病毒虽然是金标准,但耗时、费力,对早期诊断意义不大,但适于研究或疫苗研发。     

IgG在前列腺癌细胞株中的表达及意义

目的探讨免疫球蛋白(IgG)在前列腺癌发生、发展过程中的作用。方法培养雄激素依赖性前列腺癌(LNCaP)细胞株和雄激素非依赖性前列腺癌(PC-3)细胞株,采用流式细胞仪检测两种细胞株IgG蛋白表达,实时定量PCR法检测IgG mRNA表达。结果 LNCaP细胞膜及胞质IgG阳性率分别2.96%、89.22%,PC-3细胞分别为86.73%、90.99%,两者阳性表达率比较(P<0.05);LNCaP、PC-3细胞IgG mRNA相对表达量分别为1±0.37、3.08±0.15,P<0.05。结论 IgG在LNCaP和PC-3细胞中均有表达,PC-3细胞中呈高表达,提示其与前列腺癌的恶性程度密切相关。     

海南省不明原因发热病人中钩端螺旋体病的实验检测结果分析

目的了解海南省不明原因发热病人中钩端螺旋体病的感染情况。方法分别用钩端螺旋体显微镜凝集试验(MAT)、酶联免疫试验(ELISA)和聚合酶链反应(PCR)法,检测海南省2015年全省不明原因发热病人的血标本。结果共检测标本179份,其中显微镜凝集试验检出阳性标本40份,感染率为22.35%,男性感染率为21.65%(21/97),女性感染率为23.17%(19/82);菌群中有7个群能检出,分别是问号钩端螺旋体黄疸出血群、拜伦群、秋季热群、波摩那群、流感伤寒群、赛罗群、塔拉索夫群;酶联免疫试验检测阳性38份,阳性率为21.23%,其中IgM阳性6份,检出率为3.35%,IgG阳性32份,检测率为17.88%;血清学检测结果一致。PCR法检测结果均为阴性。结论海南省不明原因发热病人中存在钩端螺旋体的感染,主要型别以黄疸出血群为主。     

胶体金免疫层析法与双单克隆抗体夹心ELISA检测鼠疫F1抗原的比较

目的比较胶体金免疫层析法（GICA）和双单克隆抗体夹心酶联免疫吸附试验（DMcAbS-ELISA）检测鼠疫F1抗原的敏感性和特异性。方法用GICA试纸条和DMcAbS-ELISA平行检测308份强毒鼠疫耶尔森菌感染鼠脏器标本和327份对照鼠标本,用RIHA微量法同时检测作为参照。结果327份对照鼠鼠疫细菌学检验及GICA,DMcAbS-ELISA和RIHA的F1抗原检测均为阴性,GICA,DMcAbS-ELISA和RIHA在108cfu/mL水平上未发现与近缘假结核耶尔森菌有交叉反应;308只感染鼠样本细菌培养阳性284只,24只样本未分离到鼠疫菌;F1抗原检测GICA阳性287份,DM-cAbS-ELISA阳性280份,RIHA阳性276份,GICA阳性检出率最高93.19%,与DMcAbS-ELISA和RIHA比较,差异均有统计学意义（χ^2=5.14,9.09;P=0.016,0.001）;GICA与DMcAbS-ELISA符合率97.73%（Kappa=0.845）;GICA和DMcAbS-ELISA与RIHA符合率分别是96.43%和98.70%（Kappa=0.774,0.926）;284份细菌培养阳性鼠标本F1抗原检测GICA阳性率是98.59%,高于DMcAbS-ELISA和RIHA,差异有统计学意义（χ^2=4.17,5.14;P=0.031,0.016）;24份细菌培养阴性实验鼠标本F1抗原检测：GICA检出7份阳性,阳性率29.17%,DMcAbS-ELISA检出6份阳性,阳性率25%,RIHA检出3份阳性,阳性率12.5%,GICA高于DMcAbS-ELISA和RIHA,但差异无统计学意义（χ^2=2.25,0;P=0.125,1.000）。结论GICA检测鼠疫F1抗原敏感特异,快速简便,优于DMcAbS-ELISA和RIHA,是鼠疫快速诊断中有应用价值的检测技术。     

乙型肝炎病毒共价闭合环状DNA套式-实时荧光定量聚合酶链反应的建立

目的 建立检测HBV共价闭合环状DNA(cccDNA)的套式一实时荧光定量PCR法.方法 根据HBV cccDNA与松环DNA(rcDNA)结构上的差异,设计2对跨缺口的特异引物及1条位于负链缺口下游的特异TaqMan荧光探针.根据Plasmid-SafeTM ATP-Dependent Dnasc(PSAD)对rcDNA与cccDNA作用的不同,对模板DNA进行酶切纯化,降解reDNA,再进行套式PCR扩增,先用外引物和模板进行第一轮常规PCR,再用内引物、荧光探针和第一轮PCR产物进行实时荧光定量PCR,根据阳性参照标准品,得出待检标本定量值.结果 检测阳性参照标准品.得出该方法灵敏度可达2 lg拷贝/mL.用上述方法检测34份乙型肝炎患者血清HBV DNA阳性标本,25份血清HBVcccDNA阳性,28份外周血单个核细胞HBV cccDNA阳性.27份健康对照者血清HBV DNA阴性标本,6份HBV cccDNA阳性.对5份HBV cccDNA阳性标本扩增产物进行克隆测序,无碱基缺失、突变.与HBV不同基因型序列(A～G)比较,同源性为90.6%～99.1%,其中,与B、C基因型同源性为95.3%～99.1%,验证了方法的特异度.结论 套式-实时定量PCR法可检测乙型肝炎患者血清、PBMC中的HBV CCCDNA,且具有敏感、特异性.     

重庆万州区育龄妇女TORCH感染的调查研究

目的探讨重庆市万州区育龄人群TORCH的感染现况,为优生优育工作提供数据支撑。方法选取2016年8月～2018年7月在重庆市万州区医院进行TORCH筛查的3 975例育龄期妇女,检测其血清Anti-TORCH抗体,并分成高龄组(≥35岁)及低龄组(35岁)进行比较。结果 3 975例育龄期妇女血清IgM抗体检测阳性率分别为Anti-TOX IgM 1.45%、Anti-RV IgM 1.76%、Anti-CMV IgM 6.92%;IgG抗体的阳性率为Anti-TOX IgG 13.27%、Anti-RV IgG 92.26%、Anti-CMV IgG 98.32%、Anti-HSVⅠIgG 70.48%;Anti-HSVⅡIgG 17.56%。高龄组妇女Anti-TOX IgM、Anti-CMV IgM的阳性检出率分别为3.01%和9.58%,低龄组分别为1.21%和6.50%,差异均有统计学意义(χ~2=10.43、6.90,P均0.05),Anti-RV IgM的阳性检出率高龄组为2.08%,低龄组为1.71%,差异无统计学意义(P0.05)。高龄组妇女Anti-TOX IgG、Anti-RV IgG和Anti-CMV IgG阳性检出率分别为14.50%、91.43%、97.93%,低龄组分别为13.08%、92.39%、98.39%,差异均无统计学意义(P0.05)。高龄组Anti-HSVI IgG和Anti-HSVⅡIgG阳性检出率分别为83.24%和27.01%,低龄组分别为68.43%和16.06%,差异均有统计学意义(χ~2=49.30、35.53,P0.01)。结论本地区育龄期妇女TORCH抗体阳性率较高,应加强育龄期妇女特别是高龄备孕女性的TORCH筛查力度。     

荧光定量PCR与常规PCR检测血清乙型肝炎病毒的对比观察

目的 探讨荧光定量聚合酶链反应（FQ-PCR）与常规聚合酶链反应检测乙型肝炎病毒（HBV）敏感性的差异。方法 对105份乙型肝炎患者血清用两种方法检测HBV。结果 常规PCR对10^6-10^9,10^5-10^4和≤10^3copy/ml的血清检测结果有统计学差异。常规PCR检测10^6-10^9copy/ml的血清假阴性率仅5.0%,10^4-10^5copy/ml时假阴性率达36.8%,10^3copy/ml主要为阴性结果，但有6例为阳性或弱阳性（24.0%)。结论 常规PCR对≤10^5copy/ml的血清有较高的假阴性率，比FQ-PCR灵敏度低10-100倍，弱阳性标本FQ-PCR检测也可能出现假阴性结果。     

实时荧光PCR检测MRSA对减少儿科ICU病区医院感染的效果观察

目的观察实时荧光PCR检测耐甲氧西林金黄色葡萄球菌(MRSA)对减少儿科ICU病区医院感染的效果。方法选取2011-07~2012-06实时荧光PCR法检测的儿科ICU病区标本856份,其结果与分离培养药敏试验法的检测结果进行比较;另与2009-07~2010-06 MRSA医院感染率进行比较。结果实时荧光PCR法与分离培养药敏试验法对金葡菌检测的一致率为100%,均检测出33株金葡菌阳性株。实时荧光PCR法与分离培养药敏试验法检测MRSA的符合率为93.9%。分离培养药敏试验法检测为阴性而实时荧光PCR法检测为阳性的2株待检菌的测序结果显示为阳性。利用实时荧光PCR法检测MRSA后,医院感染率从36.4%下降到16.7%。结论实时荧光PCR法能更及时、更准确发现MRSA,从而早隔离、早治疗,减少MRSA医院感染。     

Field evaluation of a one-step dipstick assay for the diagnosis of human leptospirosis in the Seychelles

OBJECTIVE AND METHOD: To compare the response of a dipstick assay (DSA) detecting Leptospira-specific immunoglobulin M (IgM) antibodies with that of an enzyme-linked immunosorbent assay (ELISA), an indirect haemagglutination assay (IHA), the microagglutination test (MAT) and a polymerase chain reaction assay (PCR) in patients with leptospirosis confirmed by MAT alone or by MAT and/or PCR (MAT/PCR). RESULT: In 75 patients with acute leptospirosis diagnosed by MAT (respectively, 90 patients diagnosed by MAT/PCR), the response in paired early and convalescent sera was positive in 78.9% (67.9%) by DSA, 76.0% (67.8%) by ELISA, 58.7% (55.6%) by IHA, 44.0% (53.3%) by PCR, and 100% (90.0%) by MAT. In early serum only, the response in patients diagnosed by MAT (respectively by MAT/PCR) was positive in 36.0% (38.9%) by DSA, 36.0% (37.8%) by ELISA, 14.7% (18.9%) by IHA, 39.2% (48.3%) by PCR, and 53.3% (58.9%) by MAT titre > or =1:100. DSA detected the main serogroups implicated in human leptospirosis in Seychelles and demonstrated sensitivity comparable to ELISA. In 124 single sera from control subjects without overt disease, the response was positive in 4.8% by DSA, 3.2% by ELISA, 3.2% by IHA, 13.8% by PCR, 37.9% by MAT titre > or =1:100, and 2.4% by MAT titre > or =1:800, giving evidence of the frequency of both past and current subclinical infection in Seychelles and that DSA was less sensitive than MAT to detect moderate levels of leptospiral antibodies. CONCLUSION: DSA is a simple and reproducible assay well adapted to field conditions and could usefully contribute to the evaluation of leptospirosis in areas devoid of serological laboratory facilities.     

不同抗囊尾蚴抗体检测试剂盒用于囊尾蚴病血清学诊断效果评价

目的　评价不同厂家生产的4种抗囊尾蚴IgG、IgG4和IgM抗体酶联免疫吸附试验（ELISA）检测试剂盒的诊断效果,为囊尾蚴病流行病学调查和临床检测提供参考。方法　采用A品牌3种抗囊尾蚴抗体（IgG、IgG4和IgM抗体）ELISA检测试剂盒以及B品牌抗囊尾蚴IgG抗体ELISA检测试剂盒,同时检测40份脑囊尾蚴病患者、100份健康人、30份斯氏并殖吸虫病患者、17份细粒棘球蚴病患者和19份皮下或脑裂头蚴病患者血清,比较不同试剂盒检测囊尾蚴病的敏感度、特异度和假阳性率。结果　A品牌抗囊尾蚴IgG、IgG4和IgM抗体ELISA试剂盒以及B品牌抗囊尾蚴IgG抗体ELISA试剂盒检测囊尾蚴病的敏感度分别为95.00%（38/40）、87.50%（35/40）、7.50%（3/40）和75.00%（30/40）,特异度分别为98.00%（98/100）、100.00%（100/100）、100.00%（100/100）和100.00%（100/100）;A品牌抗囊尾蚴IgG抗体ELISA试剂盒检测囊尾蚴病的敏感度高于B品牌（[χ2] = 6.28,P < 0.05）,两者特异度差异无统计学意义（[χ2] = 2.01,P > 0.05）。4种试剂盒检测并殖吸虫病、细粒棘球蚴病、裂头蚴病患者血清总假阳性率分别为37.88%（25/66）、22.73%（15/66）、62.12%（41/66）和15.15%（10/66）（[χ2] = 37.61,P < 0.05）;其中A品牌抗囊尾蚴IgM抗体ELISA试剂盒检测总假阳性率最高（[χ2] = 7.56,P' < 0.008）,A品牌抗囊尾蚴IgG抗体ELISA试剂盒检测总假阳性率高于B品牌（[χ2] = 8.75,P' < 0.008）。4种试剂盒检测并殖吸虫病患者血清假阳性率分别为40.00%（12/30）、16.67%（5/30）、76.67%（23/30）和13.33%（4/30）（[χ2] = 32.88,P < 0.05）,检测裂头蚴病患者血清假阳性率分别为21.05%（4/19）、26.32%（5/19）、73.68%（14/19）和15.79%（3/19）（[χ2] = 19.97,P < 0.05）,均以A品牌抗囊尾蚴IgM抗体ELISA试剂盒检测假阳性率最高（P' 均 < 0.008）。4种试剂盒检测细粒棘球蚴病患者血清假阳性率分别为52.94% （9/17）、29.41%（5/17）、23.53%（4/17）和17.65%（3/17）,差异无统计学意义（[χ2] = 8.24,P > 0.05）。A品牌抗囊尾蚴IgM抗体ELISA试剂盒检测并殖吸虫病、棘球蚴病和裂头蚴病患者血清假阳性率分别为76.67%（23/30）、23.53%（4/17）和73.68%（14/19）（[χ2] = 14.537,P < 0.05）,其中检测棘球蚴病患者血清假阳性率最低（[χ2] = 14.537,P' < 0.014）;其他3种试剂盒检测并殖吸虫病、棘球蚴病和裂头蚴病患者血清假阳性率差异均无统计学意义（P 均> 0.05）。 结论　不同囊尾蚴病免疫学诊断试剂各有优劣;A品牌抗囊尾蚴IgG抗体ELISA试剂盒敏感度较高,但需进一步解决与其他寄生虫病的交叉反应和稳定性问题。     

Polymerase chain reaction in comparison with serological tests for early diagnosis of human leptospirosis

The aim of this study was to compare the sensitivity and specificity of polymerase chain reaction (PCR) using two primer pairs and combined with blood culture, immunoglobulin M enzyme-linked immunosorbent assay (IgM ELISA), microscopic agglutination test (MAT) and slide agglutination test (SAT) in the diagnosis of human leptospirosis. We analysed 124 serum samples: 60 from patients with confirmed leptospirosis, 20 from patients with other diseases and 44 from healthy individuals. Analysing the first serum sample collected during the first 3-8 days of disease, the sensitivities of the four tests MAT, IgM ELISA, SAT and PCR were, respectively, 69.0%, 79.3%, 72.4% and 62%. In subsequent samples, those same sensitivities were, respectively, 95.4%, 100%, 100% and 72.7% in samples collected from days 9 to 14 and 88.9%, 88.9%, 77.8% and 44.4% in those collected from days 15 to 42. The most specific method (at 100%) was PCR and the least specific (at 89.1%) was IgM ELISA. Although we found PCR to be less sensitive than the serological tests over the course of the disease, our data indicate that PCR was the most sensitive in those initial serum samples presenting no specific antibodies detectable by any of the serological methods tested. We also recommend that PCR can be used in combination with serological tests as we found that this improves the sensitivity of the diagnosis of leptospirosis in the first phase of the disease (93.1-96.5%).     

胶体金免疫层析法检测结核分枝杆菌特异性IgG/IgM抗体对结核病诊断的应用价值

目的 评价胶体金免疫层析法检测血清中结核分枝杆菌特异性IgG/IgM抗体在结核病诊断中的应用价值。方法 收集结核病患者和健康人的血清样本共332份及其背景资料,采用胶体金法检测血清中特异性结核抗体IgG/IgM,与临床诊断和细菌学检测结果比较,使用SPSS 22.0统计软件对结果进行比较分析,以P<0.05为差异具有统计学意义。结果 胶体金免疫层析法检测结核病患者中特异性结核抗体IgG/IgM的灵敏度为41.15%、特异性为91.67%,检测菌阳和菌阴结核病患者的敏感性分别为51.38%和33.77%。在全部结核病患者中,特异性结核抗体IgG/IgM检测检出率(41.15%)显著高于痰涂片法(18.84%)和痰菌培养法(36.15%)(P<0.05)。结核抗体检测、痰涂片法和痰培养法三种方法联合检测阳性率为61.54%,高于单种方法检测或其中两种方法联合检测。结论 胶体金免疫层析法检测血清中结核分枝杆菌抗体具有灵敏、特异、快速和简便等优点,可用于结核病的筛查,同时该方法也具有一定的局限性,敏感度和特异度有待进一步的提高,因此不可单独用于诊断结核病,可配合痰细菌学、影像学和临床表现等进行辅助诊断。     

散发性戊型肝炎的病原学和血清学研究

目的 研究散发性戊型肝炎（ＨＥ）患者病毒血症,粪便排病毒及抗体消长规律。方法 采用逆转录－套式－聚合酶链反应（ＲＴ－ｎｅｓｔｅｄ－ＰＣＲ）动态检测血清和粪便ＨＥＶＲＮＡ,采用ＨＥＶＯＲＦ２,ＯＲＦ３合成多肽单独和联合ＥＩＡ法动态检测血清抗－Ｈ。结果 血清ＨＥＶＲＮＡ阳性患者占７１％（４４／６２）平均持续时间为病后２０．６天（全程随访３２例）粪便ＨＥＶＲＮＡ阳性标本中有９６．２％（２５／２６）出现在     

Swine-like hepatitis E viruses are a cause of unexplained hepatitis in the Netherlands

Hepatitis E virus (HEV) infections in developed countries are recognized as an imported disease related to travel to endemic regions. However, increasing evidence suggests that HEV infection may also occur in the developed countries and that swine may act as a possible reservoir. To investigate the indigenous transmission of HEV in the Netherlands, sera from 50 blood donors and 1027 sera from patients with acute hepatitis were screened with an ELISA for HEV-specific IgG and IgM. Because the Netherlands is considered a nonendemic region, all positive ELISA results were confirmed by immunoblot to exclude false-positive results. Evidence of recent HEV infection was detected in 0% of the blood donors and 4.4% of the cases, based on combined positive IgM and IgG responses. The serodiagnosis was confirmed by a positive polymerase chain reaction (PCR) in 24 patients with hepatitis (2.3% overall, 51% of confirmed IgM+/IgG+ cases). IgG antibodies alone were detected in 4.2% of patients. We found related sequences to virus strains detected in Dutch pigs (genotype 3, 91-97% homology) in 89% of PCR-confirmed HEV patients. The detection of unique swine-like HEV sequences in 16 indigenous hepatitis patients without a recent travel history suggests that HEV is endemic in the Netherlands. We recommend including HEV tests in unexplained acute hepatitis patients, despite their travel history.     

用不同基因型戊型肝炎病毒重组蛋白建立酶联免疫吸附测定一步法

目的 以7种不同基因型和亚型的HEV重组蛋白作为包被抗原,建立HEV抗体检测ELISA一步法.方法 通过方阵滴定法确定包被抗原浓度,确定临界值,并进行敏感度、特异度和热稳定性试验.结果 7种重组抗原混合物(Mix166)最佳包被浓度为1.5 mg/L.抗-HEV IgG检测试剂的批内、批间变异系数分别为8.67%和10.85%,抗-HEV IgM检测试剂的批内、批间变异系数分别为4.56%和5.99%.一步法检测50份HEV RNA阳性血清的HEV IgG和HEV IgM抗体,阳性率均为94%.一步法检测674份健康者血清,52份抗-HEV IgG阳性,3份抗-HEV IgM阳性.一步法检测HEV墨西哥株攻击黑猩猩后收集的系列血清发现,病毒攻击后1～6周抗-HEVIgM阳性,2～76周抗-HEV IgG阳性,而进口试剂盒缺乏对抗-HEV墨西哥株IgG、IgM的反应性.结论 Mix166作为包被抗原建立的HEV抗体ELISA一步法具有较好的敏感性和特异性,可用于HEV感染的诊断.     

Development of monoclonal antibody based IgG and IgM ELISA for diagnosis of severe fever with thrombocytopenia syndrome virus infection

IntroductionSevere fever with thrombocytopenia syndrome virus (SFTSV) is a newly emerged virus that poses a great threat to human health because of high fatality rate.MethodsTo develop sensitive and specific sero-diagnostic systems for SFTSV infections, monoclonal antibodies (MAbs) against recombinant SFTSV nucleocapsid (rSFTSV-N) protein were developed by immunizing BALB/C mice with rSFTSV-N protein and fusing the spleen cells with SP2/0 myeloma cells. Three hybridoma cell lines secreting MAbs against rSFTSV-N were obtained. MAb based IgG sandwich enzyme linked immunosorbent assay (ELISA) and IgM capture ELISA systems were established by using the newly developed MAbs. One hundred fifteen clinical suspected SFTS patients serum samples were used to evaluate the newly established systems by comparing with the total antibody detecting sandwich ELISA system and indirect ELISA systems.ResultsThe MAbs based sandwich IgG ELISA was perfectly matched with that of the total antibody sandwich ELISA and the indirect IgG ELISA. IgM capture ELISA results perfectly matched with that of the total antibody sandwich ELISA while detecting eight additional positive samples missed by the indirect IgM ELISA.ConclusionsThe MAbs against rSFTSV-N protein offer new tools for SFTSV studies and our newly developed MAb-based IgG and IgM capture ELISA systems would offer safe and useful tools for diagnosis of SFTS virus infections and epidemiological investigations.     

SARS-CoV抗体实验诊断的特异性研究

为探讨严重急性呼吸综合征(SARS)病毒(SARS-CoV)抗体在SARS病原学诊断中的特异性，应用酶联免疫吸附试验(ELISA)和荧光定量RT-PCR技术检测了80例非SARS患者SARS-CoV抗体的阳性率。结果在23例健康人中，SARS-CoV-IgG抗体的阳性率为8．7％(2／23)，20例肿瘤患者中，阳性率为20％(4／20)；18例自身免疫性疾病患者中，SARS-CoV-IgM抗体和SARS-CoV-IgG抗体阳性率分别为11．1％(2／18)和22．2％(4／18)；19例系统性红斑狼疮(SLE)患者中，SARS-CoV-IgM抗体和SARS-CoV-IgG抗体阳性率分别为21．1％(4／19)和47．4％(9／19)，SARS-CoV-IgG抗体和SARS-CoV-IgM抗体同时阳性率为15．8％(3／19)。证实SARS-CoV-IgM抗体诊断SARS的特异性为92，5％；SARS-CoV-IgG抗体诊断SARS的特异性为76．25％；两种抗体同时阳性诊断SARS的特异性为96．25％。经RT-PCR证实上述抗体单项阳性均为假阳性。认为两种抗体同时测定可提高诊断的特异性，出现假阳性的原因可能与包被的抗原中存在细胞膜、胞浆、核和抗细胞核成分的自身抗体有关。