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Lu Lu Qinghui Meng Ming Cui Xiaofei Chu Shuyi Zhao Huiwen Xiao Jiali Dong 《国际放射医学核医学杂志》2016,40(2):85-90
Objective Lung cancer cells associated with radioresistance are likely to give rise to local recurrence and distant metastatic relapse,but little is known about its underlying mechanisms.In the present paper,the effects of the HPV16 E6 and HPV16 E7 oncoprotein on the radiosensitivity of lung cancer cell lines were investigated.Methods The HPV16 E6 or HPV16 E7 oncoprotein was expressed by a transient transfection with pcDNA3-HPV16 E6 or pcDNA3-HPV16 E7 expression vector.Human lung cancer H2179 cells and mouse lung cancer Lewis cells were exposed to a γ-ray radiation source,cellular survival was evaluated by using a colony formation assay.The expression of HPV16 oncoproteins E6/E7,extracellular signal-regulated kinases 1/2(ERK1/2) and AKT signaling was determined by Western blot assay.VEGF secretion was determined by ELISA.Results Both HPV16 oncoproteins E6 and E7 significantly decreased radiosensitivity of H2179 cells,associated with a promotion of the ERK1/2 and AKT phosphorylation.A decrease of reactive oxygen species(ROS) and an increase of VEGF levels were observed in the cells expressing the HPV16 oncoproteins E6 and E7.Furthermore,a similar reduction of radiosensitivity mediated by the HPV16 oncoproteins E6 and E7 was also observed in a mouse lung cancer Lewis cells.Conclusion The findings indicate that the HPV16 oncoproteins E6 and E7 negatively affects susceptibility of lung cancer cells to radiotherapy via regulation of the ERK1/2 and Akt signaling pathway and VEGF expression. 相似文献
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Biomarkers,such as chromosome aberration and micronuclei assays,prove to be reliable for facilitating clinical diagnosis in radiation accidents.In a radiation accident in India,chromosomal aberration,γ-H2AX,as well as other blood markers,were detected in accidentally exposed victims.This multi-parametric approach aided in confirming that individuals had been exposed by ionizing radiation.However,doses were impossible to estimate because of a 30-day delay in accident awareness.Exposure dose for victims was estimated using a dose-response curve previously established.Dose estimation,blood cell depletion kinetics,and no appearance of prodromal symptoms suggested that doses of exposure were low.Hematologic investigation,sampling time,and chromosome aberration scoring were all proposed according to data from the victims exposed to 60Co.Finally,knowledge regarding chromosome aberration analysis and the importance of international co-operation and assistance should be shared from this accident. 相似文献
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本刊讯 武警浙江总队医院在认真做好部队官兵医疗保障的同时,连续30年开展为驻地困难家庭送温暖、送健康、献爱心活动,赢得了群众高度赞誉.今年春节前夕,该院在去年帮扶310户困难家庭的基础上,进一步加大了帮扶力度,出资22万余元,对嘉兴市区410户困难家庭开展献爱心行动. 相似文献
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目的 初步探索皮下应用Gd-DTPA-白蛋白的阳性磁共振淋巴造影检测隐匿转移性淋巴结的价值.方法 建立腘窝隐匿转移性淋巴结新西兰兔模型,分析隐匿转移性淋巴结在平扫及皮下注射0.10 mmol/肢Gd-DTPA-白蛋白后24 h时的MRI特征,并与病理检查对照.结果 12只兔的腘窝隐匿转移性淋巴结模型成功建立,其淋巴结大小为(6.7±0.2)mm.平扫时隐匿转移性腘窝淋巴结表现为T1WI上等信号,T2WI上高信号,与正常淋巴结相似.Gd-DTPA-白蛋白增强24 h,正常淋巴结呈明显、均匀的强化,而隐匿转移性淋巴结呈不均匀强化,强化形式有环状(4枚淋巴结)、云絮状(3枚)和不规则偏心"充盈缺损"(3枚),另有2枚淋巴结不强化.在脂肪抑制T1WI像上淋巴管呈明亮高信号,转移性淋巴结的引流淋巴管明显迂曲扩张.结论 皮下应用Gd-DTPA-白蛋白的阳性MR淋巴造影可清晰显示淋巴结和淋巴管,可用于检测隐匿转移性淋巴结. 相似文献
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目的 观察补充维生素B2对急性低氧暴露小鼠外周血代谢组的影响.方法 采用随机数字表法将35只雄性昆明小鼠随机分为:正常对照组,低氧对照组,2倍、4倍及8倍维生素B2补充组,每组7只.以相应饲料(维生素B2含量分别为6 mg/kg、12 mg/kg、24 mg/kg和48 mg/kg)喂养2周后,除正常对照组外,其它各组均在模拟6000 m高度停留8 h,采集血浆,以核磁共振的方法分析其代谢组变化.结果 急性低氧暴露后,各组动物血浆代谢组在得分图中呈聚类型分布,且有先分离后同归的代谢模式变化轨迹,显示出补充不同剂量维生素B2后小鼠血浆代谢组逐渐恢复的趋势.代谢模式产生差别的原因是脂类、乳酸、丙氨酸、N-乙酰糖蛋白、谷氨酸、胆碱、牛磺酸、糖、肉碱、甘氨酸和肌酐等物质的水平发生了变化,表明相关的代谢途径发生了变化.结论 补充维生素B2使急性低氧暴露机体碳水化合物、脂肪、蛋白质代谢发生改变,而维生素B2可以改善碳水化合物代谢,并可能通过肉碱间接调节了脂肪代谢;同时还发现一些氨基酸代谢发生显著变化.
Abstract:
Objective To observe the effects of vitamin B2 supplementation on plasma metabonome of the mice which exposed to acute hypoxia. Methods Thirty-five male Kunming mice were randomly and averagely divided into 5 groups, among which the control and hypoxia control groups were fed the fodder containing 6, 12, 24, 48 mg/kg vitamin B2 respectively. All groups were exposed to simulated hypoxia environment (equivalent to 6000 meters above sea level) for 8 hours except control group. Nuclear magnetic resonance spectrometer was used to test the change of metabonome from collected plasma. Results After acute hypoxia exposure, the metabonome pattern in all groups showed clustering distribution in scores plot and the metabonome changes were along the trail from segregation to regression. These indicated a gradual recover tendency on plasma metabonome when different doses of vitamin B2 supplemented. The changes of lipids, lactic acid,alanine, N-acetyl-glycoprotein (NAC), glutamate, choline, taurine, glucose, carnitine, glycine and creatinine were among the factors contributing significantly to the difference of metabonome pattern and indicated the changes of related metabolic pathway. Conclusions The metabonomes of carbohydrate, lipid and protein of acute hypoxia mice are improved by vitamin B2 supplementation.Vitamin B2 is helpful to improve carbohydrate metabolism and indirectly accommodate lipid metabolism through carnitine. Vitamin B2 supplementation also results in the significant change of amino acid metabonome. 相似文献
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1病历简介
患者,男,43岁。因间断性排尿困难半月余,伴尿频、尿急、尿痛入院。患者于12年前因骨盆骨折合并尿道断裂在我院行尿道修补术,术后排尿功能良好。术后第3年出现排尿困难,经2次尿道扩张术后,排尿正常。查体:体温37.5℃,心、肺、腹无异常。外科情况:双脊肋角不饱满,双肾区轻微扣击痛,沿输尿管走行无压痛及扣击痛,下腹部正中可见8.0cm长的纵向愈合伤口。 相似文献
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患者男性,54岁,下腹部不适两年,尿频、尿急等症状,时有下腹疼痛,近日疼痛逐渐加重,尿液检查:少量红细胞. 相似文献
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目的 探讨改良吸入CO2法实施呼吸暂停试验的安全性.方法 共收集可疑脑死亡病例14例.采用传统方法(试验A)及改良吸入CO2法(试验B)实施呼吸暂停试验.改良吸入CO2法:在气管插管末端连接"T"型管,并分别与呼吸机管路及CO2气体管路连接.与传统方法不同,纯氧机械通气10min后不断离呼吸机,打开CO2阀门,向吸入气中混入1.5L/min CO2,1min后断开呼吸机,继续观察呼吸动作1min.于试验前、吸入纯氧后、脱机后每间隔2min(试验A)、脱机前及脱机1min后(试验B)、重新上机后5min行动脉血气分析,记录血流动力学参数,试验前及试验结束后测定血乳酸浓度.结果 14例呼吸暂停试验结果均为阳性.试验A,脱机后8min内PaCO2显著上升(>60mmHg,P<0.01),pH值下降(P<0.05),但PaO2保持在200mmHg以上;MAP轻度下降,但无统计学意义;与基础值比较,PAP显著升高(P<0.05).试验B,吸入CO2 1min后,PaCO2能有效上升至60mmHg以上或较基础值升高20mmHg,仅1例超过80mmHg;试验过程中,血流动力学参数均保持相对稳定,PAP无显著变化.两种呼吸暂停试验前后血乳酸浓度无明显变化.结论 吸入CO2法及传统方法实施呼吸暂停试验均能有效保持足够组织氧代谢.传统呼吸暂停试验过程中,由于高PaCO2时间长,存在肺动脉高压以及循环不稳定风险.吸入CO2法能有效避免该并发症,连续PaCO2监测的应用是试验安全的有效保证. 相似文献
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"生命在于运动",科学的体育锻炼有益于人体健康,而青春期更是锻炼身体的最佳时期。青春期锻炼身体对青少年来说有如下好处:第一,锻炼可以促进身体的生长发育。在青春期,青少年的身体会发生巨大的变化,逐渐完成从儿童到成人的转变。一旦青春期结束,人的身体也就基本定型了。衡量这一 相似文献
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目的 观察成熟耳石损伤后修复再生的形态学和元素变化特点,为深入认识其修复再生的规律和机理提供依据.方法 给予过载刺激(10 Gy 5 min)造成18只豚鼠耳石的损伤破坏,用扫描电镜(scanning electron microscopy,SEM)分别观察过载刺激后1 h、2 d、8 d时其椭圆囊囊斑,用X射线显微能谱分析技术(electron probe X-ray microanalysis,EPMA)测量不同形态类别的耳石元素构成.结果 过载刺激后1 h正常耳石形态消失,被大量球状物代替,2 d时球状物逐渐矿化,8 d时哑铃状耳石出现并与矿化球状物共存,此外各时期均可见各种耳石的变性.EPMA结果显示,变性耳石Ca元素含量显著增高和P元素含量显著降低.另外,球状物在由初期向成熟矿化期转化过程中,表现出P元素含量逐渐增加和Ca元素含量逐渐减少的趋势,但未达到统计学显著水平.结论 成熟耳石损伤破坏后可以修复再生,该过程中形态学的变化伴随着元素构成的变化. 相似文献