ELISA法检测不育男子精浆中抗人精浆免疫抑制因子IgG和IgA

本文首次建立了抗人精浆免疫抑制因子（ＳＰＩＦ）ＩｇＧ和ＩｇＡ的ＥＬＩＳＡ测定法，用其对５０例生育男子和１００例不育男子精浆中抗ＳＰＩＦ ＩｇＧ和ＩｇＡ进行了测定。结果显示：精浆中抗ＳＰＩＦ ＩｇＧ和ＩｇＡ的阳性率在生育男子分别为６％和４％；在不育男子分别为３４％和２８％。不育组抗ＳＰＩＦ ＩｇＧ和ＩｇＡ阳性率均显著高于生育组（Ｐ＜０．０１）。本文就抗ＳＰＩＦ ＩｇＧ和ＩｇＡ在抗生育及生殖道感染方面     

ELISA法检测不育男子精浆中抗人精浆免疫抑制因子IgG和IgA

本文首次建立了抗人精浆免疫抑制因子（ＳＰＩＦ）ＩｇＧ和ＩｇＡ的ＥＬＩＳＡ测定法，用其对５０例生育男子和１００例不育男子精浆中抗ＳＰＩＦＩｇＧ和ＩｇＡ进行了测定。结果显示：精浆中抗ＳＰＩＦＩｇＧ和ＩｇＡ的阳性率在生育男子分别为６％和４％；在不育男子分别为３４％和２８％。不育组抗ＳＰＩＦＩｇＧ和ＩｇＡ阳性率均显著高于生育组（Ｐ＜０．０１）。本文就抗ＳＰＩＦＩｇＧ和ＩｇＡ在抗生育及生殖道感染方面的可能作用进行了讨论。     

不育男性精浆酸性磷酸酶和锌与精液参数分析

目的:探讨不育男性精浆中酸性磷酸酶和锌水平与精子密度及精子活力的关系。方法:对21例正常生育男性、169例不育男性精浆中酸性磷酸酶和锌分别进行检测,并作白细胞染色计数。结果:①不论按精子密度分组或按精子活力分组,不育各组精浆中酸性磷酸酶含量明显低于正常对照组(P<0.01),但不育各组之间的差异无显著性意义(P>0.05);②不论按精子密度分组或按精子活力分组,不育各组精浆锌含量仅死精子症组低于正常对照组(P<0.05);③按精子密度分组,各组标本之间白细胞计数的差异用秩和检验分析,不育各组白细胞计数的平均秩次明显大于正常对照组(P<0.001);按精子活力分组,不育各组精浆白细胞计数均高于正常对照组(P<0.05),死精子症组除外(P>0.05)。④精浆中酸性磷酸酶及锌含量均与白细胞计数不相关(r=0.088,P=0.162;r=0.119,P=0.057)。结论:精浆中酸性磷酸酶水平下降和白细胞增多均可导致精子密度和精子活力降低,可作为男性不育的诊断指标;不育患者精浆中锌离子水平虽然低于正常对照组,但无显著性差异。     

精浆锌与精液质量关系的研究

本文对552例不育男子与207例正常男子精浆中锌的含量进行了比较研究,结果发现两组间锌含量有显著性差异(P<0.05)。在不育组中尤其以精子密度<20×10~6/ml组和精液不液化组差异显著(P<0.01和P<0.05)。无精症患者精浆锌高于正常人,但无统计学意义。输精管缺如者的锌值是正常人的3倍((?)±SD,432.9±74.5)。死精症患者精浆锌含量明显高于正常人。精子活动率低于40％时,随精子活动率的下降,锌含量有所上升(r=-0.2066,P<0.02),呈显著负相关。此外,本文还对16例输精管结扎前后的锌含量进行了比较,结果两者无差异。     

弱精子症、少弱精子症患者血清、精浆和精子锌含量分析

目的:检测弱精子症和少弱精子症患者血清、精浆和精子锌的含量,分析锌含量的变化与精子密度和精子运动之间的关系。方法:按照WHO《人类精液及精子-宫颈粘液相互作用实验室检验手册》第四版的标准进行精液质量分析,随机筛选出90例弱精子症、60例少弱精子症患者以及20例精液质量正常的生育者作为研究对象,利用原子吸收光谱法检测其血清、精浆、精子的锌含量并进行统计学分析。结果:3组间血清锌含量没有显著差异;弱精子症、少弱精子症患者精浆锌含量均显著低于正常生育者(P<0.05);少弱精子症患者精子锌含量显著高于弱精子症患者和正常生育者(P<0.01)。结论:弱精子症、少弱精子症患者精子的发生及运动功能下降可能与精浆锌含量的低下呈正相关;但过高的精子锌含量与精子的发生和运动功能的关系尚不十分明了,有待进一步研究。     

慢性前列腺炎及其不育者的精浆微量元素研究

本文对30例正常生育力者、48例慢性前列腺炎(CP)患者(有生育力的20例,无生育力的28例),测定了精浆锌、铜、锰、铁、硒、镁、钙及果糖含量。精浆锌、镁及钙含量在CP者明显减低,具有诊断价值。有生育力的CP者与无生育力的CP者间三元素含量无差异,提示这不是不育的直接原因。精浆锰、铜、铁、硒及果糖含量在各受试组差异均不显著。锌/果糖比值在CP不育者明显低于正常人。精浆中的金属元素与精液pH负相关。在有生育力的CP者,钙与精子活动度、锰与精子活动率及铜与精子数量分别呈正相关。在无生育力的CP者,硒与精子活力、铁与精子数量分别呈正相关。     

正常和不育男子精浆硒含量的测定

本文报告用氢化物.原子吸收法检测了17例正常生育力男子和51例不白男子的枯浆硒含量.结果为.正常组硒78.84±11.20ng/m], 少精组57。9]±39.76vg/ml, 死精组95。94‘43.97ng/m1。无精组74.26土14.13rig/m1.经统计学处理,少精组精浆硒水平低于对照纽,廾有显著性意义。死精组、无精组硒水平与对照组比无显著性意义。各组精于计数与精浆硒水平呈正相关.而各组的精子活力、活率与精浆中硒水平无相关性。结果表明硒与精于生成有关。低硒可造成生精障碍。     

精索静脉曲张不育患者生殖激素和精浆生化分析

目的 观察精索静脉曲张(VC)患者的生殖激素与精浆生化的关系.方法99例患者根据阴囊彩色多普勒超声诊断分为3组:VC不育组、原发性不育组和正常生育组,分别进行计算机辅助精子分析、电化学发光法测定生殖激素和分光光度法测定精浆生化.结果VC组和原发性不育组精液质量下降,睾酮(T)含量高于正常生育组,VC组酸性磷酸酶、锌低于原发性不育组和正常生育组,VC组T和锌存在负相关.结论VC可能会影响前列腺与下丘脑-垂体-性腺轴分泌功能,而两者之间的损害程度可能相关.     

精液液化不良男子与正常人精浆尿激酶活性的研究

应用琼脂糖－纤维蛋白－平板法和抗尿激酶多克隆抗体的免疫阻断法测定１５名正常生育男子和２０例精液液化不良的不育症男子精浆中尿激酶的活力。结果发现：正常生育组精浆中尿激酶的含量为８４５０±１０５０ＩＵ／Ｌ，液化不良组尿激酶的含量为６４５０±１０５０ＩＵ／Ｌ。与正常生育组比较，尿激酶活性明显降低，两组间差异有极显著性（Ｐ＜０．００１）。提示精浆中尿激酶可能与精液的液化有关联。     

精浆蛋白电泳带谱分析对梗阻性无精子症的定位诊断价值

目的：评估精浆蛋白电泳带谱对梗阻性无精子症的定位诊断价值。方法：对28例正常生育男子和21例梗阻性无精子症患者(包括14例双侧输精管结扎和7例双侧附睾尾梗阻)，采用不连续SDS-PAGE分析精浆蛋白带谱变化，并分别测定精浆总蛋白质含量。结果：正常生育男子与梗阻性无精子症者比较，虽然总蛋白质含量有差异(P＜0．05)，但精浆蛋白带谱分析基本相同。结论：精浆蛋白电泳带谱分析对梗阻性无精症的诊断及定位无临床意义。     

精浆微量元素对慢性前列腺炎患者不育的可能影响

本文在对正常人、无生育力及有生育力的慢性前列腺炎(CP)患者进行了精浆多种微量元素测定的基础上,分别对三组受试者进行了微量元素间的相关分析并对不育组与其它两组分别进行了影响生育力的因素分析.结果表明,三组中均存在锌,镁及钙之间非常显著的正相关,正常人及CP生育组尚存在铜与锌、铜与镁的显著正相关,CP生育组中存在硒与多个元素的相关性。影响CP者生育力的可能危险因素是铜和铁,保护因素是硒.     

慢性前列腺炎患者精浆果糖、微量元素与血清生殖激素水平的关系

本文对30例正常人及48例慢性前列腺炎(CP)患者进行了糖浆果糖、微量元素及血清FSH,LH及睾酮测定并进行了相关分析。结果表明CP者精浆锌、镁及钙含量较正常人明显减低,果糖含量差异不显著。CP者血清FSH、LH及睾酮水平均与正常人无明显差异。精浆果糖含量在CP者与LH显著正相关。正常人精浆锌、镁及钙分别与睾酮负相关,CP者不存在此关系。精浆铜、锰、铁及硒与血清生殖激素无关。     

精索静脉曲张不育患者的精浆生化分析

目的 探讨精索静脉曲张不育患者精浆中酸性磷酸酶、果糖、锌和α-糖苷酶水平的变化.方法 分别检测120例精索静脉曲张不育患者、180例非精索静脉曲张不育患者和36例正常男性的精浆中酸性磷酸酶、果糖、锌和α-糖苷酶含量.结果 精索静脉曲张不育组和非精索静脉曲张不育组精浆中酸性磷酸酶含量均显著低于正常对照组（P＜0.01）,但精索静脉曲张不育组和非精索静脉曲张不育组之间的差异无显著性意义（P＞0.05）;各组精浆果糖活性无显著性差异（P＞0.05）;精浆中锌和α-糖苷酶含量随精索静脉曲张程度的增加而降低,且明显低于正常对照组（P＜0.05）,但与非精索静脉曲张不育组之间的差异无显著性意义（P＞0.05）.结论 精索静脉曲张可通过某些因素引起精浆中酸性磷酸酶、锌和α-糖苷酶含量降低,从而造成男性不育.     

精索静脉曲张不育与微量元素锌和镉关系的研究

本研究应用原子吸收光谱法 (AAS)测定 18例精索静脉曲张 (VC)不育患者和 14例正常生育的斜疝和或鞘膜积液患者外周静脉血、精索静脉血、精浆中的锌 (Zn)和镉 (Cd)的含量。结果表明 :精索静脉曲张不育患者精静脉血和精浆中Cd含量较对照组明显增高 (P <0 .0 1)、而精浆中的Zn含量较对照组明显减低 (P <0 .0 1)。此外 ,其精索静脉血和精浆中Cd/Zn比值与对照组也有显著性差异 (P <0 .0 1)。提示Cd的增高可能是导致精索静脉曲张不育的原因之一     

Selenium, rubidium and zinc in human semen and semen fractions

The levels of selenium, rubidium and zinc were determined in samples of semen, seminal plasma and spermatozoa from men with suspected infertility, together with several parameters of semen quality. The proportion of whole semen selenium present in sperm increased with increasing sperm count from 0 to 40%. For rubidium 98 +/- 4% and for zinc 95 +/- 8% of the total amount in semen was contained in seminal plasma. In seminal plasma a positive correlation was found between the levels of zinc and selenium, and between the levels of zinc and rubidium, indicating that, like zinc, selenium and rubidium in seminal plasma also derive mainly from the prostate gland. Semen quality parameters, such as sperm motility, vitality, speed and morphology, were not correlated with the contents of the three elements in either whole semen or seminal plasma. As the seminal content of selenium is dependent on the proportion of prostatic secretion in seminal plasma and on the sperm count, and both factors can vary considerably, the selenium level of whole semen does not appear to be a suitable parameter for investigation of the relationship between selenium and semen quality. Provisional measurements suggest lower sperm selenium levels at abnormally low or high sperm counts.     

Comparison of zinc concentrations in blood and seminal plasma and the various sperm parameters between fertile and infertile men

The aim of the study was to examine the relationships between concentrations of zinc in blood and seminal plasma and sperm quality among infertile and fertile men. One hundred seven male (infertile group) partners of couples who were undergoing investigation for infertility with no known cause for the infertility and 103 men (fertile group) whose wives were pregnant at the time of the study were recruited. The subjects' blood and seminal plasma concentration of zinc were determined by atomic absorption spectroscopy. Except for semen volume, all the other semen parameters for the infertile men were significantly lower than those for the fertile group. The geometric means of the seminal plasma zinc concentration were significantly lower in the infertile group compared with those in the fertile group; 183.6 mg/L (range, 63-499) versus 274.6 mg/L (range, 55-420). There were no significant differences in the geometric means of the blood zinc concentration between the 2 groups. Seminal plasma zinc concentration was significantly correlated with sperm density (r = 0.341, P < .0001), motility (r = 0.253, P < .0001), and viability (r = 0.286, P < .0001). On the basis of the findings of this study and those of other reports, zinc may contribute to fertility through its positive effect on spermatogenesis.     

Human Seminal Plasma Cadmium: Comparison with Fertility and Smoking Habits/Cadmium in menschlichem Seminalplasma: Vergleich zwischen Fertilität und Rauchgewohnheiten

Cadmium, selenium and zinc were determined in seminal plasma and serum of 64 men by atomic absorption spectrometry (AAS). The mean (+/- SD) cadmium concentrations in seminal plasma and serum were 0.22 +/- 0.22 micrograms and 0.28 +/- 0.10 micrograms, respectively, but they did not correlate with each other. Smokers (n = 31) had significantly (p less than 0.01) higher serum cadmium concentrations than non-smokers (n = 31). Also seminal plasma cadmium in smokers was elevated, but a significant difference to non-smokers was only found if more than 20 cigarettes were consumed daily. No differences were found in semen quality and fertility between smokers and non-smokers. The seminal plasma cadmium had no correlation to selenium or zinc which, however, displayed a positive correlation (r = 0.852, p less than 0.001) to each other. It is concluded that smoking increases the exposure to cadmium. Although no obvious reproductive suppression was observed, heavy smoking may possibly enhance toxic effects in men under other detrimental exposures.     

生育与不育男性血清和精浆褪黑素检测及其意义

目的 :检测生育与不育男性精浆褪黑素 (MLT)浓度并探讨在男性生育中的意义。　方法 :年龄为 2 6～ 36岁的生育男性 (18例 )和年龄为 2 3～ 36岁的不育男性 (99例 ) ,其中 ,后者又分为正常精子症组 (13例 )、少精子症组(2 7例 )、弱精子症组 (31例 )、少弱精子症组 (17例 )和少弱畸精子症组 (11例 )。分别采集静脉血和精液 ,采用酶联免疫吸附实验 (ELISA)检测血清和精浆中MLT浓度。　结果 :血清MLT浓度在生育与不育男性之间无显著性差异 ,各组精浆MLT浓度均低于相应的血清值。生育组精浆MLT浓度与各不育组相比无显著性差异 ,而少弱精子症组和少弱畸精子症组MLT浓度下降较为明显 ,但尚未达到统计学意义 (P >0 .0 5 )。　结论 :本研究结果表明 ,精浆MLT可能对精子功能具有一定作用 ,其具体作用机制尚需进一步深入的研究。