慢性前列腺炎及其不育者的精浆微量元素研究

本文对30例正常生育力者、48例慢性前列腺炎(CP)患者(有生育力的20例,无生育力的28例),测定了精浆锌、铜、锰、铁、硒、镁、钙及果糖含量。精浆锌、镁及钙含量在CP者明显减低,具有诊断价值。有生育力的CP者与无生育力的CP者间三元素含量无差异,提示这不是不育的直接原因。精浆锰、铜、铁、硒及果糖含量在各受试组差异均不显著。锌/果糖比值在CP不育者明显低于正常人。精浆中的金属元素与精液pH负相关。在有生育力的CP者,钙与精子活动度、锰与精子活动率及铜与精子数量分别呈正相关。在无生育力的CP者,硒与精子活力、铁与精子数量分别呈正相关。     

慢性前列腺炎患者精浆果糖、微量元素与血清生殖激素水平的关系

本文对30例正常人及48例慢性前列腺炎(CP)患者进行了糖浆果糖、微量元素及血清FSH,LH及睾酮测定并进行了相关分析。结果表明CP者精浆锌、镁及钙含量较正常人明显减低,果糖含量差异不显著。CP者血清FSH、LH及睾酮水平均与正常人无明显差异。精浆果糖含量在CP者与LH显著正相关。正常人精浆锌、镁及钙分别与睾酮负相关,CP者不存在此关系。精浆铜、锰、铁及硒与血清生殖激素无关。     

精液质量与精浆微量元素关系探讨

作者采用 ICP 光谱法对86例不同质量的精液进行了微量元素分析。发现精浆中含有锌、铝、铁、铜、锰、硅、硼、金、银、钒、铊、硒12种微量元素和钙、磷、镁3种常量元素。其中锌、铁、铜、锰,铝5种微量元素及3种常量元素在不同精子密度的各组间的含量无显著性差异(P>0.05)。在不同精子活动力的两组间其精浆锌含量有极显著性差异(P<0.001),说明精浆中高锌可能是维持精子活动力的重要因素之一。精浆中铁及常量元素钙镁的含量与精浆锌含量成正比关系,提示精浆中铁、钙、镁的含量亦可能与精子活动力有关。     

男性血液和精液中微量元素含量与精子活力的相关性研究

目的:探讨男性血液和精液中微量元素的分布及其与男性精液参数的相关性。方法:采用BH-5100五通道原子吸收仪、YY-1001血铅仪和伟力彩色精子自动分析仪,对113例男性血液和精液进行钙、镁、铜、锌、铁、铅的测定及精液常规分析,以了解微量元素和精液参数的相互关系。结果:①血液与精液中铅、铜、锌、铁含量显著相关,其中以铜的相关系数最大;钙、镁没有相关性。②血液和精液中铅、铜、锌、钙、镁、铁6种微量元素分布存在显著性差异。③精液中钙与精子活率、直线运动精子活率、曲线速度、平均移动角度、活跃精子密度、直线速度、平均路径速度、侧摆幅度等指标显著正相关;铅与精子活率、直线运动精子活率、活跃精子密度、活动精子密度、直线运动精子密度等指标呈显著负相关;镁元素与精子活率、直线运动精子活率、直线运动精子密度呈显著负相关;精液酸碱度与钙、镁、铁3种元素呈显著负相关。④血液中的锌与精子活率和直线运动精子活率呈显著负相关,镁与精子活率、直线运动精子活率、平均路径速度呈显著负相关,铅与侧摆幅度呈显著正相关。结论:不同体液中的微量元素分布和精子的运动功能存在不同的相关性。     

锌硒宝对少弱精子症的疗效观察

精液中活动精子的比例及数量对男子的生育力起决定性的作用。精子的质量与人体必需的锌、镁、硒、镉等多种微量元素密切相关。近年来，随着生活方式的改变和环境的日益恶化，男性精子质量逐渐下降，由男性精液质量异常导致的不育症发病率逐渐升高，而作为促进精子发生、成熟、维持精子正常功能的微量元素特别是锌和硒在男性生育能力中发挥着重要的作用。本研究通过对138例患有少弱精子症的患者服用锌硒宝片剂疗效的观察，讨论锌硒宝在治疗男性少弱精子症导致的不育方面的作用。     

透析患者微量元素与炎症、氧化应激的相关性研究

目的:探讨血液透析(HD)和腹膜透析(PD)患者微量元素浓度与炎症、氧化应激之间的关系。方法:分别选择行维持性HD或PD治疗的年龄、性别等相似的患者各60例,同时选择年龄性别相似的健康人群30例作为健康对照组。抽血测定血微量元素浓度、CRP、血中抗氧化系统成分的浓度。结果:相比于对照组,HD组和PD组患者锌、铁和硒浓度显著降低,血浆铜水平显著上升,氧化产物水平显著增加,血浆过氧化氢酶、谷胱甘肽过氧化物酶显著降低,CRP水平明显高,SOD活性显著增加,差异均有统计学意义(P0.05)。相比于PD组,HD组患者锌、铁和硒浓度更低,CRP水平更高,氧化产物水平升高,两种酶的活性降低更显著,SOD活性亦显著增高,差异均有统计学意义(P0.05),不过两者血浆铜水平无显著差异。此外,CRP与硫代巴比土酸反应物质和蛋白质羰基水平正相关,与锌、硒水平负相关。血浆铁含量和丙二醛及羰基生产存在显著的负相关性。结论:长期HD或PD均会导致一些微量元素浓度明显改变从而增加氧化应激易感性及炎症水平。此外,PD患者血浆锌、硒,和铁的水平高于HD患者,他们水平越低患者的氧化应激及炎症水平越高。微量元素可能与透析患者的炎症和氧化应激存在一定的相关性。     

脑脊液微量元素与脊麻作用时效关系

临床上影响脊麻作用时间的因素很多,但至今没有肯定结论。本文对30例非脑性疾病患者施行脊麻同时进行脑脊液微量元素锌、铜、钙、镁测定,据此进行分析讨论,以探讨脑脊液微量元素含量与脊麻作用时效关系。     

精子与精浆中的微量元素,脂质及抗氧化系统

精液由精子和精浆组成,精浆是射精后精子的生存环境即代谢场所,由睾丸液、附睾液及男性各附属性腺分泌物共同组成。精子和精浆中某些成分含量的变化能客观地反映精子的功能状态,其中微量元素、脂质及抗氧化系统的改变具有重要的生理及临床意义。一、微量元素精浆中微量元素种类较多,包括锌、硒、镉、铜、铅、镁等。但与精子产生及成熟相关的元素主要是锌、镉、硒、铜,且它们各自作用也不尽相同。１．锌　锌在男性泌尿生殖系统含量很高,在成年男性的睾丸和精液中都含高浓度的锌,前列腺中锌含量比身体其它任何器官都要高［１,２］。与…     

合肥市3860例妊娠中、晚期孕妇全血微量元素检测结果分析

目的了解合肥地区孕妇全血微量元素水平,为合理补充微量元素和孕期指导提供科学依据。方法以原子吸收光谱法测定3 860例于安徽医科大学第一附属医院绩溪路门诊部产检的中、晚期孕妇血中铜、锌、钙、镁、铁5种微量元素的含量,以体检中心50例非孕期育龄女性作为对照组,分析比较各组间的微量元素含量差异,并与已发表的其他地区相关文献的检测结果进行比较。结果全部3 860例孕妇血微量元素水平检测值高于正常参考区间者占0.05%;低于正常参考区间者占25.60%,其中锌占1.74%、钙占3.47%、镁占0.05%、铁20.34%。孕妇组血铜、锌水平显著高于对照组(P0.05),血镁、铁水平显著低于对照组(P0.001),血钙水平组间无统计学差异(P0.05);与对照组比较,妊娠中、晚期血铜水平均显著升高(P0.05),妊娠中期血镁、铁水平显著降低(P0.05),妊娠晚期血锌水平显著升高(P0.05)而血镁、铁水平显著降低(P0.05)。与相关文献报道比较,本次检测结果显示孕妇血铜、锌、钙、镁水平基本正常,血铁水平随孕周增加有明显缺乏。结论合肥市城区内孕妇5种微量元素缺乏情况并不严重,除铁随孕周增加有相当程度缺乏外,其他元素基本不缺乏,与相关文献报道的地区基本一致;妊娠期间仍需重视铁元素补充,其他元素可选择合理食补;孕期有必要定期检测5种微量元素。     

膝关节液微量元素含量与骨性关节炎关节镜下Outerbridge分级关系的研究

[目的]探讨膝骨性关节炎(osteoarthritis,OA)膝关节液内微量元素的浓度与Outerbridge分级标准的关系。[方法]选择2012年1月~2014年1月本科确诊的膝关节OA患者160例,根据骨性关节炎关节镜下Outerbridge分级分4组,以40例健康体检者为对照,抽取各组膝关节液,检查关节液中铜、钙、镁、锌、铁元素的浓度。[结果]对照组与膝关节OA组关节液的铜、钙、镁、锌和铁浓度均有显著差异(P0.01)。不同Outerbridge的膝关节OA组内关节液的铜、钙、镁、锌和铁浓度均有显著差异(P0.01)。与Outerbridge分级相关的因素有关节液的钙、锌、铁和铜,其中铁为负相关(P0.05或P0.01),与镁无相关性。[结论]膝关节液中钙、锌、铁和铜的浓度变化可作为膝关节OA的诊断指标,且钙、锌、铁和铜与Outerbridge分级标准有相关性,可用作提示膝关节OA病变严重程度的指标。     

不育男子和正常生育力男子精浆微量元素的研究 (附183例精浆锌、铜、铁、硒和锰测定报告)

本文报告用原子吸收光谱法检测了49例正常生育男子和134例不育男子精浆锌,铜,铁,硒和锰的含量。结果表明,生育男子精浆锌,铁,硒含量较患少精症,无精症和死精症的不育男子含量高得多;此外,精浆锌浓度增加与精子活力有关。该研究提示:锌,铁和硒是维持正常生育功能所必需的元素。     

精索静脉曲张不育患者的精浆生化分析

目的 探讨精索静脉曲张不育患者精浆中酸性磷酸酶、果糖、锌和α-糖苷酶水平的变化.方法 分别检测120例精索静脉曲张不育患者、180例非精索静脉曲张不育患者和36例正常男性的精浆中酸性磷酸酶、果糖、锌和α-糖苷酶含量.结果 精索静脉曲张不育组和非精索静脉曲张不育组精浆中酸性磷酸酶含量均显著低于正常对照组（P＜0.01）,但精索静脉曲张不育组和非精索静脉曲张不育组之间的差异无显著性意义（P＞0.05）;各组精浆果糖活性无显著性差异（P＞0.05）;精浆中锌和α-糖苷酶含量随精索静脉曲张程度的增加而降低,且明显低于正常对照组（P＜0.05）,但与非精索静脉曲张不育组之间的差异无显著性意义（P＞0.05）.结论 精索静脉曲张可通过某些因素引起精浆中酸性磷酸酶、锌和α-糖苷酶含量降低,从而造成男性不育.     

Comparison of zinc concentrations in blood and seminal plasma and the various sperm parameters between fertile and infertile men

The aim of the study was to examine the relationships between concentrations of zinc in blood and seminal plasma and sperm quality among infertile and fertile men. One hundred seven male (infertile group) partners of couples who were undergoing investigation for infertility with no known cause for the infertility and 103 men (fertile group) whose wives were pregnant at the time of the study were recruited. The subjects' blood and seminal plasma concentration of zinc were determined by atomic absorption spectroscopy. Except for semen volume, all the other semen parameters for the infertile men were significantly lower than those for the fertile group. The geometric means of the seminal plasma zinc concentration were significantly lower in the infertile group compared with those in the fertile group; 183.6 mg/L (range, 63-499) versus 274.6 mg/L (range, 55-420). There were no significant differences in the geometric means of the blood zinc concentration between the 2 groups. Seminal plasma zinc concentration was significantly correlated with sperm density (r = 0.341, P < .0001), motility (r = 0.253, P < .0001), and viability (r = 0.286, P < .0001). On the basis of the findings of this study and those of other reports, zinc may contribute to fertility through its positive effect on spermatogenesis.     

精浆锌与精液质量关系的研究

本文对552例不育男子与207例正常男子精浆中锌的含量进行了比较研究,结果发现两组间锌含量有显著性差异(P<0.05)。在不育组中尤其以精子密度<20×10~6/ml组和精液不液化组差异显著(P<0.01和P<0.05)。无精症患者精浆锌高于正常人,但无统计学意义。输精管缺如者的锌值是正常人的3倍((?)±SD,432.9±74.5)。死精症患者精浆锌含量明显高于正常人。精子活动率低于40％时,随精子活动率的下降,锌含量有所上升(r=-0.2066,P<0.02),呈显著负相关。此外,本文还对16例输精管结扎前后的锌含量进行了比较,结果两者无差异。     

Liver cirrhosis induced by carbon tetrachloride and the effect of superoxide dismutase and xanthine oxidase inhibitor treatment.

Repeated administration of carbon tetrachloride (CCl4) induces liver cirrhosis, possibly because it involves the production of free radicals. In order to evaluate the effect of free radical scavengers such as superoxide dismutase (SOD) and allopurinol in the pathogenesis of liver cirrhosis, rats were subjected to repeated CCl4 administration with and without scavengers. Four groups of animals were studied: CCl4 plus SOD (group 1), CCl4 plus allopurinol (group 2), CCl4 alone (group 3) and olive oil (group 4, normal controls). Analysis of plasma and tissue concentrations of trace elements was performed and histopathological patterns were studied in all groups after 7 weeks of repeated intraperitoneal administration of the solutions. Plasma levels of zinc and selenium were significantly lower in all experimental groups, with reciprocal elevation of manganese and copper. Copper and manganese content in the liver tissue was significantly higher in all three experimental groups. The zinc content was elevated in groups receiving CCl4 alone (group 3) or with allopurinol (group 2). The liver selenium, however, was significantly lower in these two groups. The copper:zinc ratio for plasma was 0.78 in the control group, 1.6 in the CCl4 group, 1.3 in the allopurinol group and 1.5 in the SOD group. For liver tissue, the ratio was 0.07 for controls, 0.17 for CCl4, 0.11 for allopurinol and 0.28 for the SOD group. The changes in trace element content correlated with the severity of cellular damage observed microscopically in the liver. The higher the copper:zinc ratio, the more advanced and extensive was the microscopic evidence of liver injury after CCl4 challenge.     

Seminal lead and copper in fertile and infertile men: Blei und Kupfer im Spermaplasma bei fertilen und infertilen Männern

Lead and copper concentrations were determined by atomic absorption spectroscopy in semen from 18 fertile and 172 infertile men. Significant correlations between copper concentrations in semen and sperm concentration (r = 0.32, P less than 0.001), percentage progressive motility (r = 0.23, P less than 0.005) and normal morphology (r = 0.22, P less than 0.005) were observed, while no such correlation existed for lead. However, semen copper concentrations of infertile men (194.99 +/- 5.70 micrograms l-1) and fertile men (183.39 +/- 14.37 micrograms l-1) did not differ significantly. Mean lead concentration in semen of fertile men was 11.18 +/- 0.62 micrograms l-1 and significantly higher than lead concentration in semen of fertile men (5.61 +/- 0.53 micrograms l-1, P less than 0.006). Reinvestigation of 18 infertile men after 2 years showed a significant drop of lead concentrations in semen from 17.31 +/- 1.41 to 6.94 +/- 1.32 micrograms l-1 (P less than 0.0002), which might be related to the decreasing use of leaded petrol in the Federal Republic of Germany.     

弱精子症、少弱精子症患者血清、精浆和精子锌含量分析

目的:检测弱精子症和少弱精子症患者血清、精浆和精子锌的含量,分析锌含量的变化与精子密度和精子运动之间的关系。方法:按照WHO《人类精液及精子-宫颈粘液相互作用实验室检验手册》第四版的标准进行精液质量分析,随机筛选出90例弱精子症、60例少弱精子症患者以及20例精液质量正常的生育者作为研究对象,利用原子吸收光谱法检测其血清、精浆、精子的锌含量并进行统计学分析。结果:3组间血清锌含量没有显著差异;弱精子症、少弱精子症患者精浆锌含量均显著低于正常生育者(P<0.05);少弱精子症患者精子锌含量显著高于弱精子症患者和正常生育者(P<0.01)。结论:弱精子症、少弱精子症患者精子的发生及运动功能下降可能与精浆锌含量的低下呈正相关;但过高的精子锌含量与精子的发生和运动功能的关系尚不十分明了,有待进一步研究。     

In vitro clearance of trace elements via continuous renal replacement therapy.

OBJECTIVE: Trace element loss during continuous renal replacement therapy in patients with acute renal failure has not been quantified sufficiently. DESIGN: Trace element loss was quantified using an in vitro model of continuous venovenous hemofiltration. Bovine blood was used for the experiment, and the plasma was analyzed for its chromium, copper, selenium, manganese, and zinc content. Two different polysulfone hemodiafilters, a low-flux F8 and high-flux F70 were used, and tested at two different ultrafiltrate flow rates of 1 L/hr and 2 L/hr, respectively. Trace element concentrations in the plasma and ultrafiltrate were analyzed using inductively coupled plasma mass spectrometry. The sieving coefficient and clearance of each trace element were calculated and compared between the two hemodiafilters. SETTING: In vitro bovine model of continuous venovenous hemofiltration. PATIENTS OR OTHER PARTICIPANTS: Not applicable. RESULTS: Mean sieving coefficients of both F8 and F70 hemodiafilters were similar for each trace element. Copper, chromium, manganese, selenium, and zinc all were detected in the ultrafiltrate. Estimated trace element loss using typical trace element blood concentrations and study-derived sieving coefficients suggest that daily losses of selenium are greater than what is replenished with a standard daily trace element supplement in total parenteral nutrition. CONCLUSION: These data suggest that the degree of continuous venovenous hemofiltration clearance chromium, copper, selenium, manganese, and zinc differ between elements and that selenium and copper might need to be replaced with doses that exceed typical supplementation guidelines.     

Magnesium in human semen: possible role in premature ejaculation

Although magnesium is involved in many biological process and it is found higher levels in semen than serum, its role in human semen has not been elucidated. This investigation was conducted to evaluate the relationship between premature ejaculation and the levels of seminal magnesium. The levels of magnesium, zinc, copper, and selenium were evaluated with an atomic absorption spectrophotometer in serum and seminal plasma in 3 groups of men: (a) normal sperm parameters (15) (b) oligoasthenozoospermia (15), and genuine premature ejaculation (9). There were normal serum and semen levels of all the elements in the three groups, but significantly lower seminal plasma magnesium levels in men with premature ejaculation. The hormonal profile, body mass index (BMI) had no association with premature ejaculation. Decreased levels of magnesium gives rise to vasoconstriction from increased thromboxane level, increased endothelial intracellular Ca2+, and decreased nitric oxide. This may lead to premature emission and ejaculation processes. Magnesium is probably involved in semen transport. More research into the role of magnesium in the male physiology of reproductive tract, especially its association with premature ejaculation, is advocated.