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目的观察盐酸戊乙奎醚对小鼠胃肠动力及血浆血管活性肠肽的影响。方法健康昆明小鼠30只,体重18~24g,雌雄不限,随机分为对照组、阿托品组和盐酸戊乙奎醚组,每组10只。阿托品组腹腔注射阿托品0.3mg/kg,盐酸戊乙奎醚组腹腔注射盐酸戊乙奎醚0.3mg/kg,对照组给予等容量0.9%氯化钠溶液。3组给药15min后以营养性半固体糊0.8ml/只灌胃,30min后处死小鼠,采用放免法测定血管活性肠肽水平,计算胃内残留率。结果与对照组比较,阿托品组血管活性肠肽水平升高(P<0.05),盐酸戊乙奎醚组血管活性肠肽水平差异无统计学意义(P>0.05);与阿托品组比较,盐酸戊乙奎醚组血管活性肠肽水平下降(P<0.05)。与对照组比较,阿托品组胃内残留率升高,盐酸戊乙奎醚组胃内残留率差异无统计学意义(P>0.05);与阿托品组比较,盐酸戊乙奎醚组胃内残留率降低(P<0.05)。结论与阿托品比较,盐酸戊乙奎醚不抑制胃肠运动,其对血管活性肠肽的分泌无影响。 相似文献
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《河北医药》2012,34(5)
目的 观察盐酸戊乙奎醚对小鼠胃肠动力及血浆血管活性肠肽的影响.方法 健康昆明小鼠30只,体重18 ~24 g,雌雄不限,随机分为对照组、阿托品组和盐酸戊乙奎醚组,每组10只.阿托品组腹腔注射阿托品0.3 mg/kg,盐酸戊乙奎醚组腹腔注射盐酸戊乙奎醚0.3 mg/kg,对照组给予等容量0.9%氯化钠溶液.3组给药15 min后以营养性半固体糊0.8 ml/只灌胃,30 min后处死小鼠,采用放免法测定血管活性肠肽水平,计算胃内残留率.结果 与对照组比较,阿托品组血管活性肠肽水平升高(P<0.05),盐酸戊乙奎醚组血管活性肠肽水平差异无统计学意义(P>0.05);与阿托品组比较,盐酸戊乙奎醚组血管活性肠肤水平下降(P<0.05).与对照组比较,阿托品组胃内残留率升高,盐酸戊乙奎醚组胃内残留率差异无统计学意义(P>0.05);与阿托品组比较,盐酸戊乙奎醚组胃内残留率降低(P<0.05).结论 与阿托品比较,盐酸戊乙奎醚不抑制胃肠运动,其对血管活性肠肽的分泌无影响. 相似文献
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PVC膜离子缔合型有机碱阳离子选择电极响应规律探讨 总被引:2,自引:0,他引:2
以硅钨酸等六种未转型的定域体试剂为活性物的电极,测试十几种有机碱,并与以离子对为活性物的膜电极比较,得出定域体与待测离子缔合力强弱是决定离子缔合阳离子选择电极响应性能的主要因素的结论。提出预先筛选电活性物的方法——目视比浊法。找出未转型的硅钨酸为活性物、邻苯二甲酸二(2-乙基己基)酯为增塑剂的膜电极响应性能较佳,对某些有机碱响应的线性宽于或下限低于文献值。 相似文献
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本文报道以四苯硼酸-利多卡因作电活性物质制成了利多卡因PVC电极。并研究了膜相中活性物质的含量对电极性能的影响,活性物质含量为0.6%时,电极性能良好。电极的线性区为5×10^-2~2.5×10^-5mol/L,平均斜率为59.5,检测下限为1.4×10^-5mol/L,适用的pH范围宽,抗干扰能力强,且有较高的稳定性。电极响应快,适用于利多卡因样品的快速测定。 相似文献
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目的:在胆碱酯酶抑制剂沙林所致的犬循环衰竭模型上,观察循环衰竭前后及N受体拮抗剂美加明与M受体拮抗剂阿托品救治后,动物血液内心血管活性物质的变化情况。方法:20只健康成年雄性杂种犬,体重12~15 kg。随机分为4组,对照组、阿托品组、美加明组和阿托品与美加明联用组。肌肉注射沙林诱发循环衰竭模型,以氯解磷定10mg.kg-1为底药,观察循环衰竭前后血液内心血管活性物质的变化以及药物的影响。结果:与染毒前相比,沙林诱发犬循环衰竭时,血液中舒血管活性物质一氧化氮(NO)、前列腺环素(PGI2)和心房肽(ANP)以及缩血管活性物质血管紧张素Ⅱ(AngⅡ)、内皮素(ET)和血栓素A2(TXA2)均显著增高(P<0.01),美加明与阿托品合用治疗15 min时,上述心血管活性物质即显著下降(P<0.05),60 min时基本恢复正常,疗效均明显好于单用阿托品、美加明组。结论:血浆NO、PGI2、ANP、AngⅡ、ET和TXA2等心血管活性物质的升高水平与胆碱酯酶抑制剂沙林诱导的犬循环衰竭程度及病情转归相关,美加明与阿托品合用,抗毒作用显著增强。 相似文献
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离子色谱法测定尿碘浓度 总被引:1,自引:1,他引:1
目的:建立测定人尿样中碘离子浓度的离子色谱法。方法:采用离子色谱,在IonPacAS7柱上,用50mmol/L的硝酸为淋洗液测定尿液中碘离子的含量。检测方式为直流安培检测,工作电极为Ag电极,参比电极为Ag/Agcl电极。结果:碘离子浓度检测线性范围为50~1000μg/L(r=0.9998),平均回收率为95.8%~97.0%,相对标准偏差为2.6%~3.7%;测得的正常人尿碘浓度为100~300μg/L。结论:该方法稳定、准确、快速,可用于实际样品的测定。 相似文献
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目的:建立线性扫描伏安法(LSV)和循环伏安法(CV)等电化学方法测定头孢拉定胶囊含量的方法。方法:工作电极为多壁碳纳米管修饰玻碳电极(MWCNT/GCE),参比电极为Ag/AgC(l饱和KCl溶液)电极、辅助电极为铂丝电极。结果:在优化试验条件下,还原峰电流与头孢拉定浓度在5.0×10-6~1.0×10-4mol·L-1范围内呈良好的线性关系(r=0.9973),检测限为1.7×10-6mol·L-1,平均加样回收率为99.8%,RSD=1.02%(n=9)。结论:该方法简单、快速、灵敏,可用于头孢拉定各种剂型的质量分析。 相似文献
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S Pinzauti E La Porta P Gratteri G Papeschi V Giannellini 《Pharmaceutica acta Helvetiae》1989,64(4):125-128
A differential pulse polarographic method requiring little sample separation was developed for the determination of isosorbide-5-mononitrate in tablet dosage form with the standard addition technique and without interference from common excipients. Britton-Welford buffer (pH 3.0) was used as the supporting electrolyte, the single peak occurring at -0.36 V vs. a reference Ag/AgCl electrode. The irreversible, diffusion controlled, two-electron reduction process at the dropping mercury electrode permits a precise and accurate determination of the active ingredient in the 0.4-20 microgram/ml concentration range. 相似文献
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目的:建立一种黄柏中盐酸小檗碱含量测定的直接电位法.方法:以盐酸小檗碱(BH)为目标分子,甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂制备出盐酸小檗碱分子印迹聚合物,以此聚合物为电活性物质,制备出盐酸小檗碱玻璃涂膜离子选择性电极,采用工作曲线法测定了黄柏中盐酸小檗碱的含量.结果:当盐酸小檗碱的浓度C在1×10-6~1×10-4 mol·L-1之间与电动势E呈现良好的Nernst响应,斜率为52.44 mV/pc, 回收率为96.9%~103.6%.结论:该方法测定快速、准确、灵敏,结果满意. 相似文献
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Marcolino-Júnior LH Bergamini MF Teixeira MF Cavalheiro ET Fatibello-Filho O 《Il Farmaco; edizione pratica》2003,58(10):999-1004
The behavior of a carbon paste electrode was investigated as an amperometric detector for the determination of dipyrone by flow injection analysis (FIA). The electrode presented low cost and easy construction by simple mixing of graphite powder and mineral oil. Initially, an electrochemical study of the dipyrone oxidation at a carbon paste electrode has been developed before its use in the FIA system. The oxidation currents monitored at +0.35 V versus Ag/AgCl, were proportional to the dipyrone concentrations. Experimental parameters, such as nature of supporting electrolyte, pH of the carrier solution, flow rate, sample volume injection and probable interferents were investigated. Under the best experimental conditions selected, the calibration curve for dipyrone was linear in the concentration range from 4.91 x 10(-6) to 2.50 x 10(-4) M l(-1) (I(anodic)/microA)=0.056+81.06 [dipyrone]) with a detection limit of 2.07 x 10(-6) M l(-1). Recoveries ranged from 93.8 to 100.8% and an analytical frequency of 130 h(-1) was achieved. The proposed flow procedure has been satisfactorily applied to the determination of dipyrone in several pharmaceutical formulations. 相似文献
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Franzoi AC Spinelli A Vieira IC 《Journal of pharmaceutical and biomedical analysis》2008,47(4-5):973-977
A carbon paste electrode modified with poly(vinylpyrrolidone) (PVP) was developed for the determination of rutin. PVP enhances the adsorption of rutin on the electrode surface due to the presence of hydrogen bonding between the imide group in PVP and the hydroxyl group in rutin. The current responses in cyclic and linear sweep voltammetric experiments were investigated and an oxidation peak was observed at +0.87 V vs. Ag/AgCl. Several parameters were investigated to evaluate the performance of the modified electrode. The best analytical response was obtained employing (75:15:10%, w/w/w) graphite powder:Nujol:PVP, with an accumulation time of 10 min, scan rate of 100 mV s(-1) and 0.1M phosphate buffer solution (pH 6.0) as supporting electrolyte. The analytical curve was linear for rutin concentrations of 3.9 x 10(-7) to 1.3 x 10(-5)M (r=0.9991) and the detection limit was 1.5 x 10(-7)M. The recovery of rutin from pharmaceutical samples ranged from 98.3 to 101.7% and the relative standard deviation was 3.3% for a solution containing 7.7 x 10(-6)M rutin (n=5). This electrode was successfully applied to the determination of rutin in pharmaceutical formulations. The results obtained using the proposed modified carbon paste electrode and those obtained by the standard method are in agreement at the 95% confidence level. 相似文献
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目的 建立测定β-环糊精中有关物质α-环糊精和γ-环糊精的离子色谱方法,并用质谱进行验证.方法 采用离子色谱法,CarPac PA 200离子柱,柱温30 ℃,安培检测器,金电极为工作电极,Ag/AgCl为参比电极.高分辨质谱法采用电喷雾电离模式,离子阱扫描范围为300~1 400.结果 α-环糊精和γ-环糊精的检出限为0.2和0.5 mg·L-1,定量限分别为0.4和1.0 mg·L-1.采用离子肼质谱法确认了α-环糊精和γ-环糊精,结果与离子色谱相一致.所有样品均检出γ-环糊精,同时探讨了其限量标准,为该品种标准提高和风险监控提供了技术参数.结论 该方法快速、有效,可用于β-环糊精中有关物质的检测. 相似文献
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离子选择电极法测定三黄片中盐酸小檗碱的含量 总被引:2,自引:0,他引:2
以小檗碱溴汞酸盐为电活性物制备PVC膜离子选择电极,用以测定三黄片中盐酸小檗碱的含量。电极的线性范围1.0×10~(-3)~5.0×10~(-7)mol/L,级差56mV/pC,检测下限2.3×10~(-7)mol/L,平均加样回收率100.3%,RSD0.75%。 相似文献