冷适应建立对寒冷暴露大鼠心肌的保护作用

 摘要：【目的】依据不同强度冷刺激大鼠血浆激素水平的改变建立冷适应性模型，为研究寒冷适应性保护机制奠定基础。【方法】60只SD大鼠分成3组每组20只。对照组，4℃冷刺激4h/d组，0℃冷刺激10h/d组。高效液相检测血浆儿茶酚胺，放射免疫法检测血浆皮质醇换算成皮质酮含量。观察大鼠冷适应后寒冷暴露血浆LDH,CK-MB ,心肌髓过氧化物酶（MPO）活性。【结果】不同强度冷刺激大鼠血浆糖皮质激素、儿茶酚胺的分泌水平不同组间比较（P＜0.01），建立冷适应模型。冷适应处理大鼠寒冷暴露组血浆LDH、CK-MB和心肌MPO活性显著低于寒冷暴露组（P＜0.05）。【结论】建立了冷适应大鼠模型，冷适应能够降低寒冷暴露所造成的心肌损伤。     

柯萨奇病毒B组3型VP1蛋白的原核表达及免疫效果研究

目的:用原核细胞表达柯萨奇病毒B组3型VP1蛋白并观察其免疫效果.方法:构建原核表达质粒pET-his/VP1,转化大肠杆菌BL21(DE3)pLysS,诱导VP1蛋白的表达并纯化.BALB/c小鼠随机分为实验组和对照组.实验组腹腔注射VP1蛋白,对照组注射PBS,每3周免疫1次,共免疫3次.每次免疫后2周取血清,用微量中和试验法检测血清CVB3特异性中和抗体滴度;末次免疫后3周,每组随机取3只小鼠,用细胞计数试剂盒检测淋巴细胞增殖活性和特异性CTL杀伤活性;每组另随机抽取3只腹腔注射3LD50CVB3,第7天检测血中病毒滴度;用致死量的CVB3攻击每组剩余12只小鼠,观察免疫保护作用.结果:实验组小鼠血清中和抗体滴度随免疫次数增加而提高(P＜0.05),第3次免疫后中和抗体滴度达70.79±1.31,明显高于PBS对照组(P＜0.05);非特异性淋巴细胞增殖活性亦明显高于PBS组(P＜0.05),但特异性淋巴细胞增殖活性和CTL杀伤活性两组间无统计学差别(P＞0.05);CVB3攻击后,实验组小鼠血中病毒滴度显著降低,生存率达33.33%,而PBS组小鼠无存活,差异有统计学意义(P＜0.05).结论:VP1蛋白可显著增强小鼠的体液免疫应答水平,提高致死量CVB3感染后小鼠的生存率.     

线栓法复制昆明小鼠局灶性脑缺血再灌注模型的稳定性研究

目的: 探讨影响线栓法复制小鼠局灶性脑缺血再灌注模型稳定性的因素。方法: 60只雄性昆明小鼠按体重分为3组:A组(18-21 g)、 B 组(22-28 g)和C组(30-35 g)。线栓栓塞法制备大脑中动脉缺血再灌注模型。模型评价指标包括:PRM2激光多普勒脑血流仪检测大脑中动脉供血区的血流,按Longa标准进行小鼠运动行为评分,TTC 染色法测量小鼠脑梗死灶体积,ELISA法检测脑皮层丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果: 与 C组相比, A、B两组模型成功率高(P＜0.05),尤以B组模型成功率更高;A组死亡率显著高于B组和C组(P＜0.05);A、B两组行为学评分显著高于C组(P＜0.05),表现出明显的神经功能障碍;A、B两组小鼠脑梗死灶的体积显著高于C组 (P＜0.05),表现出明显的脑损伤,损伤侧脑皮层中MDA含量明显升高,SOD活性明显下降。结论: 小鼠体重与线栓直径良好匹配、进线深度和24 ℃左右的室温是保证造模成功的3个重要因素。脑组织脂质过氧化反应可作为脑缺血再灌注损伤的有效指标。     

丙酮酸乙酯对脓毒症小鼠血清Leptin及乳酸／丙酮酸比值、ALT和UA的影响

探讨丙酮酸乙酯(Ethyl Pyruvate,EP)对脓毒症小鼠血清Leptin、乳酸/丙酮酸比值、丙氨酸氨基转移酶(ALT)和尿酸(UA)水平的影响.建立小鼠脓毒症模型,90只昆明小鼠随机分为:假手术组(Sham组)、林格氏液组(RLS组)、EP处理组(REPS组),后两组又设立15 min、1 h、3 h、6 h组,每组10只,用全自动生化分析仪检测血清乳酸/丙酮酸比值、ALT、UA;RIA测定血清Leptin水平.结果表明:1、血清Leptin水平:REPS组后期显著高于RLS组(P＜0.05)2、乳酸/丙酮酸:与Sham组比较,RLS组与REPS组均显著升高(P＜0.05或P＜0.01);REPS组与RLS组比较各相同时间点之间无显著性差异.3、血清ALT:与Sham组比较,REPS组与RLS组均显著增高(P＜0.01或P＜0.05);REPS组在后期显著高于RLS组(P＜0.05).4、血清UA:与Sham组比较,RLS组先显著升高(P＜0.05)后降低,REPS组先降低后升高,后期显著高于Sham组(P＜0.05);REPS组与RLS组比较显著降低(P＜0.05).EP对脓毒症损伤小鼠肾功能具有一定的保护作用,并加重脓毒症早期肝脏损伤,同时能促进Leptin的释放.     

单纯心理应激小鼠模型的建立及对行为、内分泌免疫功能的影响

目的 建立Balb/c小鼠单纯心理应激模型及其评价体系,观察单纯心理应激对机体内分泌免疫功能的影响.方法 采用旁观电击实验建立心理应激模型,20只Balb/c小鼠随机分为正常对照组10只和心理应激组10只,另外准备10只小鼠仅接受足部电击,作为应激源,不进入实验处理.记录并分析正常对照组和心理应激组小鼠行为学指标,检测血清皮质酮水平,血清IL-2、IL-4含量,评价Balb/c小鼠单纯心理应激模型.结果 心理应激组小鼠旷场试验指标、血清IL-2水平较对照组有显著降低(P＜0.05);血清皮质酮、IL-4水平较对照组有显著增高(P＜0.05).结论单纯心理应激小鼠模型建立成功,该心理应激模型能激活下丘脑-垂体-肾上腺轴,抑制小鼠的细胞免疫功能.     

IL-2增强肺炎支原体P1C核酸疫苗的肌注免疫效果

目的:研究IL-2对肺炎支原体(Mycoplasma pneumoniae,Mp)P1C核酸疫苗经肌注免疫BALB/c小鼠后的免疫应答水平和免疫保护作用.方法:将P1C-IL-2核酸疫苗肌注免疫BALB/c鼠,ELISA检测疫苗免疫后56天小鼠血清IgG和IgG亚类、支气管灌洗液中SIgA、IFN-γ和IL-4的水平;用2×107 Mp菌落形成单位鼻饲感染BALB/c鼠,建立感染小鼠模型,病理切片检测Mp感染后小鼠肺部炎症病理改变;将系列10倍稀释的支气管灌洗液接种于SP4固体平板,并进行菌落计数.结果:P1C-IL-2核酸疫苗免疫组小鼠血清中的总IgG、IgG1、IgG2a、IFN-γ和IL-4水平均较P1C单基因疫苗组显著增高(P＜0.05),但两组支气管灌洗液中SIgA差异无显著性(P＞0.05).Mp感染后第1、3、6天P1C-IL-2双基因疫苗组小鼠肺组织病理评分(HPS)较P1C单基因疫苗免疫组显著增高,但支气管灌洗液中的Mp菌落数明显减少;第9天后两组HPS和Mp菌落数差异无显著性.结论:IL-2能显著增强P1C疫苗肌注的免疫保护作用和免疫应答水平,但同时在Mp感染早期激发了较重的肺组织炎症.     

免疫低下小鼠肺部肺炎链球菌感染模型的制备

目的建立免疫低下肺部肺炎链球菌感染小鼠模型。方法将配比好的FLV病毒悬浊液腹腔注射模型组小鼠,21 d后,采用滴鼻法对小鼠进行Ⅲ型肺炎链球菌接种;正常对照组给予与药液同体积的生理盐水。结果与正常对照组比较模型组小鼠毛发光泽度欠佳,竖毛现象严重,活动度逐渐减少,饮食明显减少,体质量减轻;模型组小鼠各脏器指数比正常对照组均升高,差异有显著统计学意义(P0.05),免疫低下肺部肺炎链球菌感染模型小鼠血清中IL-6、PCT表达水平均比正常对照组增高(P0.05)。结论采用FLV病毒悬浊液结合滴鼻法滴注肺炎链球菌建立的免疫低下肺部肺炎链球菌感染小鼠模型,为研究免疫低下肺部肺炎链球菌感染药物干预提供了较理想的实验模型。     

B族链球菌表面蛋白C5a肽酶系统及鼻内黏膜免疫的研究

目的 研究B族链球菌表面蛋白C5a肽酶(streptococcal C5a peptidase,ScpB)系统及其鼻内途径免疫后在小鼠血清及肺、直肠、阴道等黏膜部位特异性抗体水平,探讨ScpB蛋白黏膜免疫应答的效果.方法 在大肠杆菌BL21中表达ScpB蛋白,亲和层析法进行纯化,质谱分析法对所纯化的蛋白进行鉴定.小鼠随机分为5组,每组10只.分别为对照组、皮下30μg组、鼻内5μg组、鼻内10μg组和鼻内30μg组.用ELISA法检测小鼠血清及黏膜萃取液中特异性抗体水平;调理吞噬试验检测SepB蛋白抗血清的保护性功能.结果 成功表达并纯化了ScpB蛋白;质谱分析和蛋白质库比较证实其为B族链球菌表面蛋白C5a肽酶的可能性分值为175;ELISA显示ScpB蛋白皮下30μg及鼻内不同剂量(5 μg、10 μg、30μg)免疫后均可诱发小鼠产生高水平特异性IgG抗体(P＜0.01),30 μg剂量组IgG大于5μg及10μg剂量组(P＜0.05);鼻内不同剂量(5μg、10μg、30μg)免疫后均可诱发小鼠产生高水平的黏膜特异性IgA抗体,与对照组相比差异有统计学意义(P＜0.01);黏膜免疫组间无差异;30 μg ScpB蛋白皮下免疫后黏膜特异性IgA抗体与对照组相比差异无统计学意义(P＞0.05).SepB抗血清对细菌具有调理吞噬作用,吞噬指数为12.3,与对照组相比差异有统计学意义(P＜0.05).结论 B族链球菌表面蛋白C5a肽酶鼻内途径免疫小鼠后可在血清、肺及阴道、直肠等免疫近端和远端黏膜中产生相应高水平IgG及IgA抗体,上述ScpB抗体具有促进吞噬细胞吞噬B族链球菌的功能.     

GLS和IL-12偶联重组耻垢分枝杆菌免疫效果的观察

目的：研究携带编码人天然颗粒溶素（GLS）和小鼠IL-12基因质粒（pZM03）偶联重组耻垢分枝杆菌（ATCC607）经鼻黏膜免疫小鼠后,小鼠体内的免疫状况.方法：BALB/c小鼠36只,随机分为生理盐水、pZM03、ATCC607、卡介苗（BCG）、重组ATCC607（即携带pZM03的ATCC607）、灭活重组ATCC607组;采用滴鼻法免疫小鼠,BCG组0、14天各1次,其它组0、4、14天各1次,第0天免疫后4周处死小鼠,用ELISA检测血清中IFN-γ、IL-12、IgG2a、lL-4的分泌水平和淋巴细胞PPD诱导的IFN-γ分泌水平以及肺泡灌洗液特异性SIgA的水平,用MTS法检测免疫小鼠脾淋巴细胞增殖情况.结果：重组ATCC607血清中IFN-γ、IL-12水平与BCG组无明显差异但明显高于其他组（P＜0.05）,IgG2a水平重组ATCC607组高于BCG组和其他组（P＜0.05）,各组间IL-4水平差异无统计学意义.重组ATCC607、BCG、灭活重组ATCC607及ATCC607组用PPD均可诱导小鼠脾淋巴细胞增殖,各组间差异无显著意义,但与生理盐水和pZM03组间差异有显著意义（P＜0.05）.重组ATCC607组PPD诱导淋巴细胞IFN-γ明显高于其它组（P＜0.05）,与BCG组比较无显著差异.重组ATCC607等含菌组经鼻黏膜免疫可产生黏膜特异性SIgA.结论：重组ATCC607经鼻黏膜免疫小鼠后,机体特异性免疫特别是细胞免疫和黏膜免疫增强,为重组ATCC607治疗结核病的研究奠定了基础.     

熊果酸对SCG-7901胃癌荷瘤小鼠肿瘤的抑制作用及肠道菌群的影响

目的 探讨熊果酸对SCG-7901胃癌荷瘤小鼠肿瘤的抑制作用及肠道菌群的影响.方法 KM小鼠随机分为正常对照组、模型组、环磷酰胺组、熊果酸低剂量组、熊果酸中剂量组、熊果酸高剂量组,每组各20只.皮下移植构建SCG-7901胃癌荷瘤小鼠模型,环磷酰胺组和熊果酸组小鼠分别腹腔注射环磷酰胺和不同剂量熊果酸,1次/d,连续给药15d.末次给药24 h后,检测肿瘤组织形态学、抑瘤率,流式细胞术测定外周血CD4+、CD8+T细胞亚群含量,计算CD4+/CD8+比值.测定肠道菌群含量,并进行PCR-DGGE分析.结果 与模型组比较,环磷酰胺组小鼠瘤重显著降低(P＜0.05);与环磷酰胺组比较,熊果酸组瘤重显著降低,呈浓度依赖性(P＜0.05);与正常对照组比较,模型组小鼠外周血CD4+T含量及CD47CD8+比值显著降低(P＜0.05);与模型组比较,环磷酰胺组小鼠外周血CD4+T含量及CD4+/CD8+比值显著升高(P＜0.05);与模型组比较,熊果酸高剂量组小鼠外周血CD4+T含量及CD4+/CD8+比值均显著升高(P＜0.05);与环磷酰胺组比较,熊果酸高剂量组小鼠外周血CD4+T和CD8+T含量及CD4+/CD8+比值升高,但差异无统计学意义(P＞0.05);与正常对照组比较,模型组小鼠粪便中双岐杆菌和乳酸杆菌含量显著降低(P＜0.05);与模型组比较,环磷酰胺组小鼠粪便中双岐杆菌和乳酸杆菌含量显著降低,大肠埃希菌和肠球菌含量显著升高(P＜0.05);与环磷酰胺组比较,熊果酸组小鼠粪便中双岐杆菌、乳酸杆菌含量显著增加,大肠埃希菌和肠球菌含量显著降低,呈浓度依赖性(P＜0.05);与正常对照组比较,模型组小鼠肠道菌群多样性显著减少;与模型组比较,环磷酰胺组小鼠肠道菌群多样性减少,熊果酸组小鼠肠道菌群多样性显著增加,熊果酸组小鼠肠道菌群结构相较于环磷酰胺组更接近正常对照组.结论 熊果酸可抑制SCG-7901胃癌荷瘤小鼠肿瘤生长,抑瘤效果与环磷酰胺相当,且不良反应低,同时熊果酸还可有效调节因肿瘤导致的肠道菌群多样性减少,其中提高机体免疫功能可能是熊果酸抑瘤和调节肠道微生态的机制之一.     

SIgA基础与临床研究进展

分泌型免疫球蛋白A(SIgA)是人类黏膜免疫的主要抗体,由双体IgA[IgA(d)]、J链和分泌片(SC)共价结合构成。J链和IgA(d)由局部活化B细胞产生,SC由黏膜上皮细胞合成;SC与J链共价结合后与IgA(d)形成二聚体结构即SIgA。SIgA与多种疾病的发生、发展密切相关。最新研究表明,SIgA与慢性鼻窦炎。反复呼吸道感染、消化道感染、泌尿生殖系统感染、肝胆疾病、癌症局部免疫、口腔尖周病、缺铁性贫血、偏头痛和银屑病等疾病都有密切的关系;环境和大气污染可导致人体SIgA水平低下,局部免疫力下降。调节SIgA水平有益于改善临床疾病黏膜免疫状况。     

Downregulation of the immune system in low-quality child care: the case of secretory immunoglobulin A (SIgA) in toddlers

Does the experience of stress during child care lead to downregulation of the immune system, in particular in low-quality care? Saliva was collected from 68 toddlers attending center or family child care at home and at child care, and assayed for secretory IgA (SIgA). Caregiver sensitivity was used as an index of quality of care and was observed during three videotaped episodes of 10 min. Diurnal patterns of SIgA showed a steep fall in the morning followed by a flattening out. SIgA was not associated with type of care, but lower caregiver sensitivity was associated with lower SIgA levels in both types of care. Quality of child care is associated with a non-specific secretory component of children's mucosal immunity with well established protective effects against upper respiratory infections.     

壳聚糖递送的结核基因疫苗诱导的肺部黏膜免疫及在抗结核免疫保护中的意义

诱导肺局部黏膜免疫对于早期控制结核分枝杆菌(Mycobacterium tuberfulosis)十分关键.在我们过去构建的结核基因疫苗pHSP65pep有效诱导脾脏IFN-γ+Th1细胞应答的基础上,为更好诱导呼吸道和肺局部黏膜免疫,采用天然生物多糖壳聚糖(chitosan)作为黏膜递送介质递送结核基因疫苗.制备壳聚...     

High Prevalence of Influenza Specific Antibody Secreting Cells in Nasal Mucosa

Secretory immunoglobulin A (SIgA) provides the first line of defence against pathogens initiating infection via the mucosal route, e.g. the influenza virus. The aim of this study was to examine the basal level of influenza-specific antibody-secreting cell (ASC) in the local mucosa of the upper respiratory tract. Nineteen patients scheduled for tonsillectomy were enrolled for the study, and they had not experienced influenza during the previous year. Tonsils, blood, oral fluid and a nasal biopsy were sampled, and the basal levels of ASC and antibodies (Abs) were determined. We found low numbers of influenza-specific ASC in the blood and tonsils, but there were about 10-100 times higher numbers of specific ASC in the nasal mucosa tissue despite no recent influenza exposure. Thus, the basal level of influenza-specific ASC in the mucosa of the respiratory tract may be important in the protection against influenza infection.     

梅毒螺旋体黏附素Tp0751 核酸菌影的构建及免疫原性研究

目的:构建梅毒螺旋体(Tp)黏附素Tp0751 的重组真核大肠埃希菌菌影(EBG)并检测其在免疫鼠中的免疫原性,为探讨新型梅毒疫苗奠定基础。方法:构建pcDNA3.1(+) / Tp0751 真核表达载体,将其装载入已构建的空EBG 中,形成重组核酸菌影pcD/ Tp0751-BG,计算装载率;将核酸菌影转染鼠源性巨噬细胞RAW264.7,Western blot(WB)鉴定目的蛋白表达。将雌性BALB/ c 鼠随机分为A(PBS)、B(空EBG)、C(空pcDNA3.1)三个对照组和D(pcD/ Tp0751)、E(pcD/ Tp0751-BG)、F(pcD/ Tp0751-BG+rTp0751)三个实验组,各组间隔两周肌注免疫共三次,检测特异性血清IgG 及生殖道黏膜SIgA、小鼠脾细胞增殖水平和分泌IFN-γ水平。结果:重组真核质粒对菌影的装载率为76.1%;WB 显示此转染细胞能有效表达重组目的蛋白。D、E、F 实验组小鼠特异性血清IgG 与生殖道SIgA 效价均随免疫次数增加而增加,各时间点均显著高于三个对照组(P<0.01),于末次加免后第8 周达到峰值,此时F 组IgG 与SIgA 效价分别为1 :102 400 与1 :12 800;首次加免2 周后,E、F 组均显著高于D 组(P<0.01);末次加免2 周后,F 组显著高于E 组(P<0.01)。末次加免后第8 周,D、E、F 组的刺激指数(SI)值与IFN-γ水平均分别显著高于三个对照组(P<0.01);E、F 组均分别显著高于D 组(P<0.01);F 组分别均高于E 组(P<0.05)。结论:Tp0751 真核质粒菌影具有良好的免疫原性,在小鼠体内诱生了有效的系统和黏膜的体液应答以及系统细胞免疫应答;异源加免较同源加免免疫效果更好。     

Effect of diclazuril on intestinal morphology and SIgA expression in chicken infected with Eimeria tenella

Secretory immunoglobulin A (SIgA), as a vital actor involving in the mucosal immunity, plays a key role in defending a variety of pathogenic infections, such as bacteria, viruses and parasites. Eimeria tenella is an obligate intracellular apicomplexan parasite contacting with the digestive tract mucosa and specially parasitizes chicken caecum, causing a severe form of coccidiosis. Coccidiosis is currently mainly controlled using chemotherapeutic agents. Diclazuril, a classic coccidiostat, was used widely in the poultry industry. Because of the rising problem of drug resistance, it is therefore crucial to understand the pattern of the SIgA expression in the action of diclazuril against E. tenella. In this study, the intestinal morphology in the caecum was analyzed by haematoxylin-eosin (HE) staining, and the SIgA expression was examined by immunohistochemical technique. At the same time, the duodenum, jejunum and ileum tissues have also been evaluated. HE staining results showed that E. tenella infection caused severe damage characterized by structural disorder, haemorrhage, inflammatory cell infiltration, serous and fibrinous exudation in chicken caecum and invisible damage in the duodenum, jejunum and ileum. With the treatment of diclazuril, the damage in the caecum was alleviated obviously. Immunohistochemical analysis demonstrated that the SIgA level in the infected group was increased in the duodenum (p?p?p?p?E. tenella and provided a cure for coccidiosis by improving the immune function in chickens.     

Effect of Intranasal Administration of Lactobacillus casei Shirota on Influenza Virus Infection of Upper Respiratory Tract in Mice

In mice administered Lactobacillus casei strain Shirota (LcS) intranasally, potent induction of interleukin 12, gamma interferon, and tumor necrosis factor alpha, which play a very important role in excluding influenza virus (IFV), was evident in mediastinal lymph node cells. In this model of upper respiratory IFV infection, the titers of virus in the nasal wash of mice inoculated with 200 μg of LcS for three consecutive days (LcS 200 group) before infection were significantly (P < 0.01) lower than those of mice not inoculated with LcS (control group) (10^{0.9 ± 0.6} versus 10^{2.1 ± 1.0}). The IFV titer was decreased to about 1/10 of the control level. Using this infection model with modifications, we investigated whether the survival rate of mice was increased by intranasal administration of LcS. The survival rate of the mice in the LcS 200 group was significantly (P < 0.05) greater than that of the mice in the control group (69% versus 15%). It seems that the decrease in the titer of virus in the upper respiratory tract to 1/10 of the control level was important in preventing death. These findings suggest that intranasal administration of LcS enhances cellular immunity in the respiratory tract and protects against influenza virus infection.     

Influenza virus infection of the murine uterus: a new model for antiviral immunity in the female reproductive tract

Secretory IgA (S-IgA) mediates local immunity to influenza virus in the murine upper respiratory tract and may play an important role in local immunity to various microorganisms in the female reproductive tract as well. Although the presence of IgA in cervicovaginal or uterine secretions has been correlated with immunity to a number of pathogens, there has been no direct demonstration of the mediation of uterine antiviral immunity by S-IgA. Influenza virus, although not a normal pathogen of the reproductive tract, was used to develop a model for the investigation of mucosal immunity in the uterus. PR8 (H1N1) influenza virus injected into the ovarian bursa of BALB/c mice grew well, with peak titers between days 3 and 5. Intravenous injection of polymeric IgA anti-influenza virus monoclonal antibody before or 30 min after viral challenge protected mice against viral infection. We believe this work to be the first direct demonstration of S-IgA-mediated antiviral uterine immunity. It provides a model for further investigation of immunity in the female reproductive tract.     

重症胰腺炎大鼠肠道免疫功能改变及精氨酸的调节作用

目的：探讨重症急性胰腺炎（Severe Acute Pancreatitis，SAP）大鼠肠道黏膜免疫功能变化及L-精氨酸（L-Arg）对SAP大鼠肠道黏膜免疫功能的影响。方法：成年、雄性Wistar大鼠随机分为胰腺炎组、假手术组和L．精氨酸治疗组。分别于造模后24、48和72小时取标本检测：鲎试剂法检测大鼠门静脉血内毒索水平、免疫组化方法检测并计数小肠黏膜固有层内CD3、CD4、CD8阳性T淋巴细胞百分数、放射免疫法检测盲肠内容物中分泌型IgA（sIgA）含量。结果：SAP组大鼠各时段门静脉血内毒素水平显著升高，回肠末段黏膜固有层CD3^＋、CD4^＋T淋巴细胞百分数明显减少，CD^＋／CD8^＋T淋巴细胞比值降低，盲肠内容物sIgA含量明显降低；与SAP组比较，L-精氨酸组大鼠各时段门静脉血内毒素水平明显降低，回肠末段黏膜固有层CD3^＋、CD4^＋T淋巴细胞百分数明显增加，CD4^＋／CD8^＋T淋巴细胞数比值升高，盲肠内容物SIgA含量增加。结论：SAP大鼠早期即发生肠黏膜免疫功能明显下降，可能是导致肠道内毒素移位的主要原因，L-精氨酸可以改善SAP大鼠肠道黏膜免疫功能，降低SAP大鼠肠道内毒索移位发生。