无机中空纳米粒与纳米管在药物/基因递送中的应用

相对于纳米材料的大小、形状及成分，无机纳米材料的生物相容性主要由表面功能化决定，对无机中空纳米材料进行适当的表面修饰可以减小其毒性；纳米管、纳米壳和中多孔纳米粒由于其中空及多孔结构，以及表面易功能化特点而成为极有吸引力的药物/基因载体；无机纳米材料的生物学效应和其在光、电及物理学方面综合的优良性能引起了人们极大兴趣，吸引了大量的相关研究。本文主要讨论了中空及多孔纳米材料在纳米医学特别是药物/基因递送应用方面近期的研究进展。     

红外驱动的动态褶皱图案:一种实现智能材料表面的有效途径

响应性表面微纳米图案可以通过外界刺激动态调控表面微纳米结构,从而实现对材料表面性能的动态调控。其中,利用材料表面不稳定性构筑褶皱是制备表面微纳米图案的通用方法,然而如何实现微纳米褶皱图案的动态调控仍然面临挑战。上海交通大学姜学松研究团队在刺激响应性高分子表面方面开展的研究工作取得了一系列阶段性成果。最近该团队提出了一种简单、普适的制备具有近红外光响应性的动态微纳米表面褶皱图案的策略,实现了对材料表面微纳米结构的多重刺激响应性调控,为构建智能材料表面开辟了新途径。     

细胞响应纳米结构表面特性的行为和机制

纳米结构的表面特性与细胞行为密切相关。近20年来,纳米尺寸表面构建和检测技术的飞速发展,提供了多种精确构建纳米材料和观测的方法,这些技术在生物医药方面的广泛应用,促使"细胞-纳米结构"的研究迅速发展。在此探讨纳米结构表面特性对细胞行为的影响,归纳"细胞-纳米结构"中可能发挥作用的机制;纳米材料表面几何学结构对于细胞行为的影响,细胞行为包括黏附、增殖、分化等。     

中华人民共和国卫生部纳米生物技术重点实验室简介

我国对纳米科技发展十分重视，国家“十五”863计划设立了纳米生物技术专题和纳米材料与微机电系统的重大专项。国家“973”计划、国家自然科学基金委员会、中国科学院也都设立了重大专项基金，我国还成立了“国家纳米科学中心”。     

中华人民共和国卫生部纳米生物技术重点实验室简介

我国对纳米科技发展十分重视，国家“十五”863计划设立了纳米生物技术专题和纳米材料与微机电系统的重大专项。国家“973”计划、国家自然科学基金委员会、中国科学院也都设立了重大专项基金，我国还成立了“国家纳米科学中心”。     

中华人民共和国卫生部纳米生物技术重点实验室简介

我国对纳米科技发展十分重视，国家“十五”863计划设立了纳米生物技术专题和纳米材料与微机电系统的重大专项。国家“973”计划、国家自然科学基金委员会、中国科学院也都设立了重大专项基金，我国还成立了“国家纳米科学中心”。     

中华人民共和国卫生部纳米生物技术重点实验室简介

我国对纳米科技发展十分重视，国家“十五”863计划设立了纳米生物技术专题和纳米材料与微机电系统的重大专项。国家“973”计划、国家自然科学基金委员会、中国科学院也都设立了重大专项基金，我国还成立了“国家纳米科学中心”。     

中华人民共和国卫生部纳米生物技术重点实验室简介

我国对纳米科技发展十分重视，国家“十五”863计划设立了纳米生物技术专题和纳米材料与微机电系统的重大专项。国家“973”计划、国家自然科学基金委员会、中国科学院也都设立了重大专项基金，我国还成立了“国家纳米科学中心”。     

中华人民共和国卫生部纳米生物技术重点实验室简介

我国对纳米科技发展十分重视，国家“十五”863计划设立了纳米生物技术专题和纳米材料与微机电系统的重大专项。国家“973”计划、国家自然科学基金委员会、中国科学院也都设立了重大专项基金，我国还成立了“国家纳米科学中心”。     

中华人民共和国卫生部纳米生物技术重点实验室简介

我国对纳米科技发展十分重视，国家“十五”863计划设立了纳米生物技术专题和纳米材料与微机电系统的重大专项。国家“973”计划、国家自然科学基金委员会、中国科学院也都设立了重大专项基金，我国还成立了“国家纳米科学中心”。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.