3T3细胞对传代表皮细胞生长影响的研究

 为探讨快速增殖表皮细胞的方法,我们采用3T3滋养细胞培养法,对传代表皮细胞生长的情况进行研究.结果表明:在3T3成纤维细胞存在时,即使低密度接种表皮细胞,表皮细胞也能以单个细胞为克隆,以单层形式生长,增殖迅速,融合成膜片时间缩短,细胞数量大,克隆形成效率高,与对照组比较均呈显著差异(P<0.05).这将为表皮细胞培养和移植救治大面积Ⅲ°烧伤开拓前景.     

3D-Ultraschall (3D-US) in der Diagnostik von Mammaherdbefunden

ZusammenfassungHintergrund Untersucht wurde, ob der 3D-US die Beurteilung von Mammaherdbefunden verbessern kann.Material und Methoden 60 Patientinnen wurden mit 2D- und 3D-US (Logiq 9 der Fa. GE, 14 MHz, multiplanare Rekonstruktionen in 3 Ebenen) untersucht. Die Herdläsionen wurden sonographisch nach BIRADS klassifiziert. Als Referenzmethode stand in allen Fällen das Ergebnis der histopathologischen Untersuchung zur Verfügung.Ergebnisse Bei den 38 malignen und 22 benignen Läsionen ergab sich mit der 2D-US eine Sensitivität/Spezifität von 92/81%, mit der 3D-US von 97/72% und bei der Kombination von 2D- und 3D-US von 97/81%. Bei malignen Tumoren zeigt sich im 3D-US eine sternförmige echoarme Formation mit Retraktion des umgebenden Gewebes.Schlussfolgerung Der 3D-US ermöglicht die mehrdimensionale Darstellung von Mammaherdbefunden, in der als Malignitätskriterium die Retraktion des umgebenden Gewebes eindrucksvoll zur Darstellung kommt.     

[P-CH_3-~3H]-梭曼的合成

本文合成了〔P-CH_3-~3H〕-1,2,2-三甲基丙氧基甲膦酰氟(〔=P-CH_3-~3H〕-梭曼).放射比度1.4ci/mmol,放射化学纯度95%以上.     

阻断PI3K信号通路显著抑制成骨前体细胞MC3T3-E1的分化

目的：研究PI3K信号通路对成骨前体细胞MC3T3-E1分化的调控作用。方法：首先通过Western印迹试验检测到PI3K信号通路参与成骨细胞分化的调控。应用PI3K激酶的特异性抑制剂LY294002药物阻断PI3K信号通路的活化，通过碱性磷酸酶(ALP)和von Kossa染色观察成骨前体细胞MC3T3-E1分化的改变。结果：Western印迹试验显示，成骨细胞分化过程中PI3K信号通路明显活化。阻断该信号通路的活化显著抑制成骨细胞碱性磷酸酶的活性，同时降低成骨细胞体外形成骨结节结构的能力。结论：PI3K信号通路参与了成骨细胞分化的调控，而这种调控作用是成骨细胞分化所必需的。     

FHL3研究进展

4个半LIM结构域蛋白3(four and a half LIM domain protein 3,FHL3)是LIM蛋白质超家族的一个成员,主要存在于骨骼肌中.它含有4个半LIM结构域,并通过这些LIM结构域与肌分化因子MyoD、肌动蛋白、转录因子MZF-1、细胞周期调节因子CDC25B等多种蛋白质发生相互作用,从而对成肌细胞分化、细胞骨架结构、骨骼肌形成以及某些基因的表达起到重要的调控作用.目前,FHL3的功能及其作用机制尚未阐明,深入开展FHIS的研究将可能为人类治疗某些疾病提供新思路.     

电离辐射对巨核细胞与白介素3及其受体基因表达的影响

目的 探讨辐射损伤对巨核细胞增殖和细胞周期的影响及其调控因子IL-3及其受体（IL-3Rα）表达的变化。方法 利用MTT法检测辐射损伤后Dami细胞的增殖作用，并通过流式细胞仪检测细胞周期的变化。用RT-PCR方法检测辐射后Dami细胞IL-3和IL-3Rα表达水平的变化。结果Dami细胞在受到不同剂量7射线照射后均发生增殖抑制，并发生明显的G0，G1期阻滞。辐射损伤5、10和15Gv后，IL-3和IL-3Rα在mRNA水平表达均显著低于对照组（P〈0．05），其变化趋势是随照射剂量增加而表达逐渐降低。结论不同剂量辐射所致造血功能障碍与巨核细胞增殖抑制、细胞周期改变和调控因子IL-3和IL-3Rα的异常表达有关。     

琥珀酰亚胺[2,3-~3H]丙酸酯对乙酰胆碱酯酶的标记

琥珀酰亚胺[2,3-~3H]丙酸酯是一种有效的酰化试剂,可以标记乙酰胆碱酯酶并且保留较高的酶活性。它也适用于其它蛋白质、多肽和伯胺化合物的标记。此试剂已在我所合成,现在可以得到。     

模拟微重力条件下奥金肽对MC3T3-E1前成骨细胞增殖的影响

目的：研究模拟微重力（simulated microgravity,SMG）条件下奥金肽（osgentide, OST）对小鼠前成骨细胞MC3T3-E1增殖功能的影响。方法在正常细胞培养环境下,利用MTT法检测6种OST化合物对MC3T3-E1细胞增殖的影响,从中筛选出有效作用浓度的化合物,进一步利用 MTT 实验及流式细胞术分别检测 SMG 条件下1 nmol／L OST5对MC3T3-E1细胞的增殖效应及细胞周期的分布。结果在正常细胞培养环境下,1 nmol／L OST5对MC3T3-E1细胞的增殖具有显著促进作用（P＜0．01）。 MTT实验结果表明,与正常细胞培养环境相比,MC3T3-E1细胞在SMG条件下其增殖受到显著抑制。在SMG条件下,用1 nmol／L OST5处理MC3T3-E1细胞（ OST-SMG）3 d,流式细胞术检测结果表明,SMG促使更多的MC3T3-E1细胞进入G1期。1 nmol／L OST5处理MC3T3-E1细胞后S期比例比SMG条件下显著提高（P＜0．05）,表明OST5能够促进DNA合成。结论在SMG条件下,OST5能够促进成骨细胞的增殖,为研究OST5对模拟微重力相关的骨丢失防治提供了理论依据。     

红色糖多孢菌M合成3-脱氧-3-羰基-赤酮酸内酯B的限速因子研究

目的探讨红色糖多孢菌KR6基因突变体生物合成3-脱氧-3羰基-赤酮酸内酯B（DOEB）产量显著降低的原因。方法以KR6基因突变体M菌株为研究对象,采用相对实时荧光定量RT-PCR技术、抗原-抗体检测技术和高分辨LC-MS分析技术等,分别从基因的转录水平、翻译水平以及聚酮化合物水平等层面研究了DOEB的生物合成量。结果红色糖多孢菌M菌株与出发菌株A226相比,两者聚酮合酶（PKS）基因簇在转录水平和翻译水平上的表达差异均非造成DOEB产量降低的限速因子;而DOEB的前体转化能力却大幅度降低,因此,DOEB合酶的活力降低是造成生物合成DOEB产量降低的关键因素。结论 DOEB合酶的活力是生物合成DOEB的限速因子,本研究为进一步探讨利用基因工程途径提高新酮内酯类化合物产量奠定了基础。     

Susceptibility effects in hyperpolarized 3He lung MRI at 1.5T and 3T

Purpose

To compare susceptibility effects in hyperpolarized ³He lung MRI at the clinically relevant field strengths of 1.5T and 3T.

Materials and Methods

Susceptibility‐related B₀ inhomogeneity was evaluated on a macroscopic scale by B₀ field mapping via phase difference. Subpixel susceptibility effects were quantified by mapping T. Comparison was made between ventilation images obtained from the same volunteers at both field strengths.

Results

The B₀ maps at 3T show enhanced off‐resonance effects close to the diaphragm and the ribs due to susceptibility differences. The average T from a voxel (20 × 4 × 4) mm³ was determined as T = 27.8 msec ± 1.2 msec at 1.5T compared to T = 14.4 msec ± 2.6 msec at 3T. In ventilation images the most prominent effect is increased signal attenuation close to the intrapulmonary blood vessels at higher B₀.

Conclusion

Image homogeneity and T are lower at 3T due to increased B₀ inhomogeneity as a consequence of susceptibility differences. These findings indicate that ³He imaging at 3T has no obvious benefit over imaging at 1.5T, as signal‐to‐noise ratio (SNR) was comparable for both fields in this work. J. Magn. Reson. Imaging 2009;30:418–423. © 2009 Wiley‐Liss, Inc.     

3D-FIESTA和3D-FSE对于跟腱损伤的诊断价值

目的：研究闭合性跟腱撕裂患者的MRI影像特点,比较其在不同MRI序列上的表现,探讨新序列在跟腱撕裂中的诊断价值。方法分析2011年6月-2013年6月间在我院就诊的41例跟腱损伤患者的MRI表现。将41例患者分为A、B两组。A组25例,行常规序列扫描（T1WI＋T2WI＋STIR）,B组16例,行常规序列（T1WI＋T2WI＋STIR）及新序列（3D-FIESTA＋3D-FSE）扫描。MRI检查后,A组11例、B组8例行手术治疗,并将其影像资料与手术结果进行比较,比较A、B组的诊断效能。结果跟腱部分撕裂的MRI表现为跟腱增粗,跟腱内条状或片状高信号。完全撕裂的MRI表现为跟腱损伤处完全分离,断端回缩或完全交错。A组诊断完全撕裂10例均行手术治疗,手术结果9例为完全撕裂。B组诊断完全撕裂7例均与手术证实符合。结论MRI对于诊断跟腱撕裂具有重要价值,新序列（3D-FIESTA,3D-FSE）能提高诊断跟腱完全撕裂的正确率及部分撕裂的检出率。     

EDTA-K3致血小板假性减少症3例

近年来,随着全自动血细胞分析仪的普及,提高了血细胞分析的速度,使全血细胞计数检测结果的精密度和准确度明显提高.实验室血细胞分析的标本留取方式也发生改变,普遍采用静脉血EDTA 抗凝.EDTA盐是国际血液学标准化委员会(ICSH)1993年建议用作血液分析的抗凝剂,使得血液不凝固并保持红细胞、白细胞、血小板体积形态不发生改变,但EDTA盐有时又能促使或诱导血小板发生聚集从而导致血液分析仪做血小板计数时出现假性偏低的现象,即EDTA依赖性血小板假性减少症(EDTA-dependent pseudothrombocytopennia, EDTA-dependent PTCP).我们在临床检验工作中,已发现有EDTA 依赖性假性血小板减少症的病例,检测时需要结合临床、排除干扰因素、选择科学可行的检测方法,才能正确报告血小板计数结果.     

Dynamic renal scanning using 99mTc-MAG-3 in man

^99mTc-labelled mercapto-acetyltriglycine (MAG-3) has recently been proposed as an ortho-iodohippurate (OIH) substitute for dynamic renal imaging. Experience with MAG-3 is, however, still limited and its biokinetics are not completely known. Moreover, most of the published studies have used HPLC purified MAG-3 which is inpractical for routine clinical work. In this study a commercially available kit of MAG-3 which does not require high performance liquid chromatography (HPLC) is used in 10 normal subjects and 15 patients with renal disease. The results obtained lead to the following conclusions:

Human erythrocyte band 3 (EPB3) polymorphism: Analysis of blood and bloodstains by an immunodetection method and frequency of the EPB3* Memphis variant

Summary The erythrocyte band 3 (EPB3) variant, band 3 Memphis (EPB3*Memphis), was detected by immunoblotting with a monoclonal antibody to the 41 kDa cytoplasmic N-terminal domain of band 3 without protease treatment of erythrocytes. EPB3*Memphis was also detected by immunoblotting from 3-month-old bloodstains subjected to -chymotrypsin treatment. A population genetic study using this method indicated that the EPB3 variant would be useful for forensic work in Japan, since the frequency of this variant in Japanese (Wakayama prefecture) is relatively high (0.159).     

Comparison of 3D TOF-MRA and 3D CE-MRA at 3 T for imaging of intracranial aneurysms

Purpose

To compare 3 T elliptical-centric CE MRA with 3 T TOF MRA for the detection and characterization of unruptured intracranial aneurysms (UIAs), by using digital subtracted angiography (DSA) as reference.

Materials and methods

Twenty-nine patients (12 male, 17 female; mean age: 62 years) with 41 aneurysms (34 saccular, 7 fusiform; mean diameter: 8.85 mm [range 2.0–26.4 mm]) were evaluated with MRA at 3 T each underwent 3D TOF-MRA examination without contrast and then a 3D contrast-enhanced (CE-MRA) examination with 0.1 mmol/kg bodyweight gadobenate dimeglumine and k-space elliptic mapping (Contrast ENhanced Timing Robust Angiography [CENTRA]). Both TOF and CE-MRA images were used to evaluate morphologic features that impact the risk of rupture and the selection of a treatment. Almost half (20/41) of UIAs were located in the internal carotid artery, 7 in the anterior communicating artery, 9 in the middle cerebral artery and 4 in the vertebro-basilar arterial system.All patients also underwent DSA before or after the MR examination.

Results

The CE-MRA results were in all cases consistent with the DSA dataset. No differences were noted between 3D TOF-MRA and CE-MRA concerning the detection and location of the 41 aneurysms or visualization of the parental artery. Differences were apparent concerning the visualization of morphologic features, especially for large aneurysms (>13 mm). An irregular sac shape was demonstrated for 21 aneurysms on CE-MRA but only 13/21 aneurysms on 3D TOF-MRA. Likewise, CE-MRA permitted visualization of an aneurismal neck and calculation of the sac/neck ratio for all 34 aneurysms with a neck demonstrated at DSA. Conversely, a neck was visible for only 24/34 aneurysms at 3D TOF-MRA. 3D CE-MRA detected 15 aneurysms with branches originating from the sac and/or neck, whereas branches were recognized in only 12/15 aneurysms at 3D TOF-MRA.

Conclusion

For evaluation of intracranial aneurysms at 3 T, 3D CE-MRA is superior to 3D TOF-MRA for assessment of sac shape, detection of aneurysmal neck, and visualization of branches originating from the sac or neck itself, if the size of the aneurysm is greater than 13 mm. 3 T 3D CE-MRA is as accurate and effective as DSA for the evaluation of UIAs.     

红色糖多孢菌A226-YA突变体构建及产物分析

目的：用红色糖多孢菌（前称糖多孢红霉菌）KR6突变体合成酮内酯类化合物3-脱氧-3-羰基-红霉内酯B，同时，KR6突变体不合成红霉素。方法：以红色糖多孢菌A226基因组DNA为模板，用重叠PCR技术扩增KR6酶域中Tyr2699密码子TAC突变为A1a密码子GCC的1000bpDNA序列，克隆到载体pWHM3上构建同源重组质粒pWHM3-YA。将pWHM3-YA转化到A226中，并整合到红霉素合成基因座，经染色体二次重组后筛选TAC突变为GCC的突变体A226-YA，再进行突变体A226-YA产物分析。结果：通过染色体同源重组，构建了红色糖多孢菌突变菌株A226-YA，Zabspec Fab质谱分析结果显示A226-YA突变体合成了3-脱氧-3-羰基-红霉内酯B，而没有检测到红霉素。结论：突变Tyr2699能有效失活KR6，但3-脱氧-3-羰基-红霉内酯B的产量较低。     

3D-FIESTA-C与3D-TOF-MRA 序列在血管压迫三叉神经痛中责任血管显示的比较研究

目的：比较三维双激发平衡式稳态自由进动序列（3D‐FIESTA‐C）与三维时间飞跃法磁共振血管成像（3D‐TOF‐MRA）在血管压迫三叉神经痛中责任血管显示的应用价值。方法回顾性分析行微血管减压术（M VD ）的60例三叉神经痛患者的磁共振资料,所有患者均采用3D‐T O F‐M RA和3D‐FIES T A‐C序列扫描,从原始及重建图像综合评判患侧三叉神经脑池段与周围血管的空间关系,以术中镜下所见为金标准,对比分析3D‐TOF‐MRA和3D‐FIESTA‐C序列预判接触血管的效能。结果判定是否存在责任血管,3D‐TOF‐MRA和3D‐FIESTA‐C序列灵敏度分别为85．7％、89．3％,特异度为75．0％、100％,准确率为85．0％、90．0％（P＝1．000）。在责任血管中,3D‐TOF‐MRA和3D‐FIESTA‐C序列判定责任动脉灵敏度分别为94．1％、88．2％（P＝0．244）,而责任静脉灵敏度为0．00％、88．2％（P＝0．009）。结论3D‐FIESTA‐C与3D‐TOF‐MRA序列均能有效显示责任血管,3D‐FIESTA‐C对责任静脉的显示优于3D‐TOF‐MRA ,可作为术前病因诊断的有力补充。     

3D-imaging of the knee with an optimized 3D-FSE-sequence and a 15-channel knee-coil

Objectives

To evaluate the clinical usefulness of an optimized 3D-Fast-Spin-Echo-sequence (3D-SPACE) in combination with a 15-channel knee-coil for 3D-imaging of the knee at 3 T.

Methods

15 volunteers and 50 consecutive patients were examined at 3 T with fat-saturated moderately T2-weighted 3D-SPACE (Voxel-size (VS): 0.6 mm × 0.5 mm × 0.5 mm/acquisition-time (AT) 10:44 min) using a 15-channel knee-coil. Flip angle optimization and radial k-space reordering were applied. Signal- and contrast-to-noise-ratios (SNR, CNR) were compared to non-optimized 3D-SPACE (8-channel knee-coil) and conventional 2D-FSE (VS: 0.4 mm × 0.4 mm × 3 mm/total AT: 12 min). Two radiologists independently rated depiction of internal knee structures and assessed detection and depiction of cartilage and meniscus abnormalities compared to conventional 2D-FSE-sequences. Sensitivity and specificity were calculated for a subgroup with arthroscopy as reference standard. Statistical analysis was performed with paired t-tests, confidence intervals and weighted-κ-coefficients.

Results

SNR and CNR particularly of fluid/cartilage of optimized 3D-SPACE were significantly higher (p < 0.05) than of the non-optimized 3D-sequence and conventional 2D-sequence. Blurring and image inhomogeneity were reduced in the optimized sequence. The thin slice-thickness was beneficial for depiction of problematical anatomical structures such as meniscal roots. 3D-SPACE showed significantly higher diagnostic confidence (p < 0.05) for diagnosis of cartilage lesions of the femoral trochlea. Overall sensitivity and specificity of 3D-SPACE and 2D-FSE for cartilage lesions was 82.3%/80.2% and 79.4%/84.2% and 100%/86.4% and 92.3%/81.8% for meniscus lesions.

Conclusions

Optimized 3D-SPACE provides significantly higher signal and contrast compared to conventional 2D-FSE, particularly for fluid and cartilage, leading to improved diagnostic confidence, particularly in problematic areas, such as the femoral trochlea.     

磁共振3D-SPACE、3D-Ture FISP序列对踝关节韧带成像的比较研究

目的 比较三维可变反转角快速自旋回波(3D-SPACE)序列、三维真稳态进动快速成像序列(3D-Ture FISP)在踝关节韧带的成像质量.方法 20例健康志愿者和15例患者在1.5T磁共振上行3D-SPACE、3D-True FISP序列成像.在正常组中比较韧带影像质量、信噪比(SNR)、对比信噪比(CNR);在患者组中比较对损伤韧带的显示能力.结果 3D-SPACE序列比3D-True FISP序列的韧带SNR、CNR更高.3D-SPACE序列比3D-True FISP序列有更好地显示踝关节正常韧带及损伤韧带的能力,但在发现损伤韧带的数量上未见明显差异.结论 相比3D-True FISP序列,3D-SPACE序列能更好地显示踝关节正常和损伤韧带的解剖结构.     

趋化因子受体CXCR3 mRNA在银屑病患者皮损部位的表达

目的:了解CXCR3 mRNA在银屑病皮损部位的表达水平及其与银屑病区域严重性指数(PASI)的关系。方法:应用RT-PCR法检测了33例银屑病患者皮肤中CXCR3 mRNA的表达水平,设30例健康对照;将检测结果与PASI进行了相关性分析。结果:银屑病皮损部位真皮CXCR3 mRNA表达水平为1.44±0.67,明显高于对照(0.59±0.29,P<0.01)及非皮损部位皮肤(1.00±0.75,P<0.01),但与PASI之间无相关性;银屑病非皮损部位真皮该受体的表达水平也显著高于对照组(P<0.05)。结论:CXCR3参与了银屑病真皮的病理变化。