平脂胶囊的质量标准

目的： 制定平脂胶囊质量控制标准． 方法： 用ＴＬＣ法鉴别成品中何首乌、川芎、蒲黄、赤芍及党参，用ＨＰＬＣ法测定何首乌及成品中大黄素含量． 结果： 五批样品ＴＬＣ鉴别均为阳性，何首乌药材及平脂胶囊大黄素含量均值分别为０．１２％ ， ０．５９５ ｍ ｇ·ｇ－ １． 结论： 经５批样品检验，该质量标准稳定可行．     

葛根的提取工艺研究

目的：通过对葛根的提取工艺的研究，确立葛根的最佳提取条件。方法：采用正交试验法研究葛根总黄酮的提取工艺，以葛根总黄酮的含量为指标，考察了药材粒径、溶媒浓度、用量、提取时间和次数等５个因素。结果：葛根总黄酮的最佳提取工艺条件为Ａ１、Ｂ１、Ｃ２、Ｄ３、Ｅ３，即以原药材饮片加１０一的７０％乙酸，回流提取７次，每次０．５ｈ。结论：葛根最佳提最工艺条件的确定为大生产提供了科学依据。     

乌头炮制品煎煮前后生物碱含量变化的初步研究

采用薄层扫描法（ＴＬＣＳ）测定乌头炮制品（制川乌、创草乌）煎煮前后乌头生物碱含量变化及其在复方的煎煮液中含量，结果表明制川乌和制草乌在常规水煎煮２．５ｈ后，乌头生物碱量降低２～５倍和１．５～４倍。复方水煎液中乌头生物碱（乌头碱、中乌头碱、次乌头碱）含量为０．００５３％～０．０３９７％。     

抗病毒新药万乃洛韦在水溶液中的降解动力学研究

目的：为获得万乃洛韦（ｖａｌａｃｙｃｌｏｖｉｒ，ＶＡＣＶ）在水溶液中降解的详细、完整、系统的动力学资料。方法：采用ＨＰＬＣ法（高效液相色谱法）及经典恒温法。结果：ＶＡＣＶ在水溶液中的水解为假一级动力学反应；在水溶液中ＶＡＣＶ既受Ｈ＋催化，又受ＯＨ－催化；酸催化系数ＫＨ＋为５×１０－３（ｈ－１·ｍｏｌ－１·Ｌ），碱催化系数ＫＯＨ－为３×１０４（ｈ－１·ｍｏｌ－１·Ｌ），ｋＯＨ－是ＫＨ＋的６×１０６倍，表明ＶＡＣＶ在水溶液中主要受ＯＨ－催化水解。ＶＡＣＶ在水溶液中的最稳定ｐＨ即（ｐＨ）ｍ为２．１；在ｐＨ２．１、ｐＨ７．２和ｐＨ８．９（ｕ＝０．３）的水解反应活化能（Ｅａ）分别是９４．５６、６７．８３和６６．７２（ＫＪ．ｍｏｌ－１），在室温（２５℃）下的有效期（ｔ０．９）分别为２８２．３、０．３和０．１天；在ｐＨ１．１的水溶液中ＶＡＣＶ水解速率常数随离子强度（ｕ）的增加而增大；相反，在ｐＨ７．２的水溶液中，ＶＡＣＶ水解速率常数随离子强度增加而降低。结论：ＶＡＣＶ在胃液ｐＨ条件下很稳定；如制成液体制剂可调ｐＨ为２．１。     

三蛇胆川贝糖浆中麻黄碱的鉴别与含量测定

用ＴＬＣ法和ＨＰＬＣ法对三蛇胆川贝乐中麻黄碱进行定性鉴别和含量测定。含量测定采用μＢｏｎｄａｐａｋＣ１８柱,以乙腈－－水－磷酸－十二烷基硫酸钠（３８：６２：０．１：０．５）为流动相,检测波长２０７ｎｍ,回收率为９６．９％）,ＲＳＤ为１．３８％。     

单硝酸异山梨酯缓释片的释放度及相对生物利用度

对试制的单硝酸异山梨酯缓释片与德国麦克乐制药公司（ Ｍｅｒｃｋｌｅ Ｇｍｂ Ｈ） 缓释胶囊剂进行体外释放度、体内血浓测定比较;体外含量测定采用 Ｈ Ｐ Ｌ Ｃ 法,体内采用 Ｇ Ｃ 法。体内数据按口服单室模型,经计算机处理求得药动学参数：缓释胶囊： Ｋａ ＝ ０ ．２５０ ｈ － １ , Ｋｅ ＝ ０ ．１３７９ ｈ － １ , Ｔｍ ａｘ ＝ ５ ．３６ ｈ , Ｃｍ ａ ｘ ＝ ３５１ ．９８ ｎｇｍｌ, Ａ Ｕ Ｃ０ →∞ ＝５３８２ ．７９ ｈ·ｎｇｍｌ , Ｆ Ｉ＝ １ ．８６ 。缓释片： Ｋａ ＝ ０ ．２４７９ ｈ － １ , Ｋｅ ＝ ０ ．１４０３ ｈ － １ , Ｔｍ ａｘ ＝ ５ ．３５ ｈ , Ｃｍａ ｘ ＝ ３７７ ．０９ ｎｇｍｌ , Ａ Ｕ Ｃ０ →∞ ＝ ５７２８ ．５１ ｈ·ｎｇｍｌ , Ｆ Ｉ＝ １ ．９７ 。研制的单硝酸异山梨酯缓释片对德国麦克乐制药公司缓释胶囊相对生物利用度为（１０６ ．２８ ±３ ．４３） ％ 。     

雾化吸入支气管扩张剂对慢性阻塞性肺疾病患者肺功能的影响

目的：为评价雾化吸入支气管扩张剂对稳定期慢性阻塞性肺疾病（ＣＯＰＤ）患者肺功能及相关参数的影响。方法：４７例患者随机分为Ａ组（１５例）、Ｂ组（１６例），分别测定基础肺功能。Ａ组吸入溴化异丙托品０．５ｍｇ，Ｂ组吸入沙丁胺醇５ｍｇ，Ｃ组吸入沙丁胺醇５ｍｇ，Ｃ组吸入沙丁胺醇５ｍｇ加溴化异丙托品０．５ｍｇ，于第１，１１，２１，２２ｄ，测吸药后３０ｍｉｎ，１，２，４，５，６，７，８ｈ的肺功能参数（ＦＥＶ１，     

利用废弃玻璃电极研制铁离子选择电极测定血清铁

１　材料与方法１．１材料：ｐＨＳ－３Ｃ型酸度计（上海第二分析仪器厂）;２３２型甘汞电极（上海电光器材厂）;选择口部破损但Ａｇ／ＡｇＣ１电极及连结完好的废弃玻璃电极。 内参比液：称取ＦｅＣｌ３０．４１ｇ,ＫＣ１０．１９ｇ加去离子水２５０ｍｌ;废液：０．１ｍｍｏｌ／Ｌ ＮａＮＯ３溶液。称取ＮａＮＯ３０．８５ｇ加去离子水至１００ｍｌ,此为０．１ｍｏｌ／Ｌ贮备液,可置冰箱保存。稀释１０００倍即为０．１ｍｍｏｌ／ＬＮａＮＯ３溶液;１．０ｍｍｏｌ／Ｌ ＦｅＣｌ３标准溶液：称取ＦｅＣ１３．０．１６２４ｇ定溶于１…     

解热抗炎Ⅰ号口服质量控制方法的研究

目的：利用ＴＬＣ和ＨＰＬＣ对解热抗炎Ⅰ号口服液中有效成分大黄素进行定性、定量分析。方法：用ＴＬＣ法对成分大黄素和薄荷进行定性分析，以ＨＰＬＣ法在ＯＤＳ－Ｃ１８柱上以甲醇－０．１％高氯酸（２０：８０）为流动相测定其含量。结果：大黄素回收率为９６．８９％（ＲＳＤ＝０．９６％），检测范围为０．０５～０．８μｇ／ｍＬ，ｒ＝０．９９９ ５，可以作为该制剂的质控方法。结论：所用定性、定量方法快速简便、灵敏度高     

中药蒲公英的质量标准研究

用ＨＰＬＣ法测定蒲公英中咖啡酸和绿原酸的含量。采用 ＨＰ Ｈｙｐｅｒｓｉｌ Ｃ_１８色谱柱,２００ ｍｍｘ ２． １ｍｍ,５μｍ,流动相：甲醇－磷酸盐缓冲溶液（ｐＨ＝４． ２） ２３： ７７,检测波长：３２３ ｎｍ,柱温：４０℃,流速：１．０ ｍＬ／ｍｉｎ。该法咖啡酸平均回收率 ９９．８０％,ＲＳＤ ２．１４％;绿原酸平均回收率９９．５４％,ＲＳＤ ２．６９％。本法稳定可靠,为药典建立蒲公英药材的质量标准提供了依据。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.