运动训练对去卵巢大鼠生物力学性能及骨代谢指标的影响

目的探讨运动训练对去卵巢大鼠生物力学性能及骨代谢指标的影响。方法选取30只雌性SD大鼠,按照随机数字表法分为正常对照组、模型对照组、运动组,每组10只,其中模型对照组、运动组进行去卵巢处理,正常对照组仅取出卵巢周围的脂肪组织。建模成功后正常对照组、模型对照组大鼠在笼内自由活动,而运动组大鼠给予适当的运动训练。在运动训练结束后对比3组大鼠的骨密度(BMD)、骨矿物质含量(BMC)、股骨生物力学性能指标、血清Ca、血清P、抗酒石酸酸性磷酸酶(STR-ACP)、骨钙素(BGP)含量。结果正常对照组BMD和BMC显著高于模型对照组,BMD显著高于运动组(均P0.05)。运动组BMD高于模型对照组(P0.05)。运动组最大荷载、弹性荷载、破坏载荷、刚性系数、能量吸收显著高于正常对照组和模型对照组(均P0.05)。正常对照组最大荷载、弹性荷载、能量吸收显著高于模型对照组(P0.05)。运动组血清Ca含量明显低于正常对照组和模型对照组,而正常对照组血清Ca含量明显低于模型对照组(均P0.05);运动组血清P含量明显高于正常对照组和模型对照组,正常对照组血清P含量明显低于模型对照组(均P0.05);模型对照组血清STR-ACP含量明显高于运动组和正常对照组,运动组血清STR-ACP含量明显高于正常对照组(P0.05);模型对照组血清BGP含量显著低于正常对照组和运动组(均P0.05)。结论运动训练能明显提高去卵巢大鼠BMD,增强股骨生物力学性能,改善骨代谢指标,有利于降低骨质疏松症的发生率。     

中等强度上坡运动对去势大鼠椎体骨质疏松的影响

目的探讨中等强度上坡运动对去势大鼠椎体骨质疏松的影响。方法将48只3月龄雌性大鼠按体重随机分成假手术组、、雌激素组和运动组,每组12只,假手术组仅切除卵巢附近一团脂肪,其余组均切除双侧卵巢。术后按不同要求灌胃给药或运动,于第12周末处死大鼠,对各组大鼠第3腰椎行生物力学性能检测。结果与模型组比较,运动组结构力学指标和材料力学指标均明显提高(P<0.05或P<0.01),与雌激素组比较,运动组结构力学指标和材料力学指标均无明显变化(P>0.05)。结论中等强度上坡运动能减少去势大鼠椎体骨量丢失,减缓骨质量的降低速度,可有助于防治骨质疏松。     

不同期慢性肾脏病患者骨密度及骨代谢生化指标关系

目的观察不同阶段慢性肾脏病（CKD）患者的骨密度（BMD）及骨代谢情况．方法选取100例慢性肾脏病患者,根据MDRD公式计算估计肾小球滤过率（eGFR）,按eGFR不同分为3组：B组（eGFR为30～59ml／min）、c组（eGFR为15～29ml／min）、D组（eGFR〈15ml／min）,并设同年龄非CKD患者作为对照组（A组）,应用DEXA骨密度仪测定L2～L4正位及股骨颈正位BMD,4组间进行血肌酐（SCr）、全段甲状旁腺激素（iPTH）、超敏C反应蛋白（hs—CRP）、碱性磷酸酶（ALP）及BMD比较。结果不同组之间BMD及骨代谢指标有明显差异,CKD患者早期BMD已有所下降,股骨比腰椎骨质丢失更明显。BMD与iPTH呈负相关,iPTH与SCr、血磷（P）、ALP、hs—CRP呈正相关,与eGFR呈负相关,而与血钙（Ca）不相关。结论随着CKD病程的进展,BMD不断下降,肾性骨病发病增加,应引起足够重视,并于CKD早期积极干预。     

去卵巢大鼠股骨和腰椎骨生物力学特性及骨矿物含量的变化

目的比较去卵巢大鼠股骨和腰椎的骨生物力学特性及骨矿物含量的不同。方法10.5月龄未交配的雌性SD大鼠40只,随机分为10.5月龄基础对照组;13月龄假手术组;13月龄去卵巢10 w组;16月龄假手术组;16月龄去卵巢22 w组。大鼠行双侧卵巢去除术后,然后灌喂生理盐水5 m l.kg-1.d-1。大鼠处死后采用三点弯曲试验测定股骨和压缩试验测定腰椎的骨生物力学指标,然后分别测量股骨和腰椎的骨矿物质含量。结果去卵巢大鼠腰椎的骨破坏荷载、破坏应力、弹性模量均明显降低及骨钙、骨磷、骨镁含量均明显降低,但股骨生物力学参数和矿物质含量均无明显变化。结论去卵巢大鼠腰椎的骨生物力学和骨矿物含量均明显降低,而股骨变化不明显。     

仙珍骨宝对维甲酸致大鼠骨质疏松的影响

目的 探讨仙珍骨宝对维甲酸致大鼠骨质疏松的影响.方法 4月龄未交配的SPF级雌性SD大鼠24只,随机分成正常对照组、维甲酸组、仙珍骨宝组,持续给药28 d.大鼠处死后取股骨进行骨密度的测量,取第4腰椎进行生物力学参数测量,取胫骨上段测定骨形态计量学参数.结果 仙珍骨宝组腰椎生物力学指标均高于维甲酸模型组(P<0.05),但股骨骨密度和胫骨上段骨形态计量学参数与维甲酸模型组无明显差别(P>0.05).结论 仙珍骨宝可对抗维甲酸致大鼠腰椎生物力学性能的降低,但对骨密度和骨形态计量学参数无明显影响.     

大鼠骨基质形态增龄性改变及其与骨代谢指标的相关性研究

目的　研究大鼠增龄过程中骨基质形态计量改变规律及与骨代谢指标相关性。　方法　 3、9、15个月龄雌性SD大鼠各 10只。第 4腰椎及右侧胫骨上段作形态计量学分析。物理密度法测定股骨及腰椎骨密度 (BMD)。第 3腰椎作抗压缩试验。自动分析仪测定血清钙、磷 ,放免法测定1,2 5 (OH) 2 D3 。　结果　与 3月龄组比较 ,15月龄组第 4腰椎骨小梁体积减小 4 3 3% (P <0 0 1) ,胫骨上段骨小梁体积减小 2 8 0 % (P <0 0 1) ,骨形成表面降低 17 2 % (P <0 0 5 ) ,骨吸收表面增加39 8% (P <0 0 1) ,腰椎、股骨BMD分别下降 7 7% (P <0 0 1)和 7 3% (P <0 0 1)。骨小梁体积与腰椎BMD、最大载荷、血清 1,2 5 (OH) 2 D3 呈明显正相关 (r =0 6 84、0 972及 0 86 6 ,P <0 0 0 1)。血清 1,2 5 (OH) 2 D3 与胫骨骨形成表面及矿化沉积率呈明显正相关 (r =0 5 87,r =0 6 2 8,P <0 0 1)。　结论　 15月龄雌性SD大鼠骨形成减弱 ,骨吸收增加 ,骨量减少 ,骨结构改变 ,生物力学性能降低。骨形态计量学指标与腰椎BMD和骨生物力学指标有明显的相关性。     

补肾活血方对去势大鼠骨质疏松的影响

目的观察补肾活血方对去势大鼠的血生化、骨密度、生物力学及病理学的影响,评价补肾活血方防治去势大鼠骨质疏松的疗效。方法取36只雌性大鼠随机分为对照组、假手术组和补肾活血组。补肾活血组与对照组大鼠行卵巢切除术,假手术组行假手术处理,均饲养3个月后行病理检查确认造模成功。造模成功后各组大鼠分别给予0．9％氯化钠注射液、0．9％氯化钠注射液、补肾活血中药灌胃,3个月后处死取血分离得到血清,分别检测血清雌激素（E：）、碱性磷酸酶（ALP）和骨钙素（BGP）。取大鼠腰椎、股骨测量腰椎的骨密度（BMD）和最大承载力、行股骨病理学观察。结果与对照组比较,补肾活血方组大鼠血清E：升高,差异有统计学意义（P〈0．01）;骨形成指标ALP、BGP上升,差异有统计学意义（P〈0．05）。与对照组比较,补肾活血组大鼠腰椎BMD及最大承载力均升高,差异有统计学意义（P〈0．05）。HE染色病理组织形态观察发现补肾活血方组大鼠骨小梁稍细,无明显变薄,排列尚整齐并连接成网,密度、面积尚正常,部分区域骨小梁间隙略增大,较对照组有明显改善。结论补肾活血方能够提高去势大鼠雌激素水平,促进骨形成,增加骨量,提高骨组织的力学性能,改善骨组织状况,达到防治骨质疏松的疗效。     

补肾中药组方对去卵巢大鼠骨生物力学的影响

目的 研究补肾中药组方（CKD）对去卵巢大鼠骨生物力学的影响.方法 60只雌性大鼠随机分为假手术组、卵巢切除（OVX）模型组、OVX＋CKD高剂量组、OVX＋CKD中剂量组、OVX＋CKD低剂量组和OVX＋雌二醇（E2）组,每组10只.3个月后,采用双能X线骨密度测定仪测定骨矿物质密度（BMD）并比较不同剂量CKD对OVX大鼠骨生物力学的影响.结果 模型组大鼠BMD有明显的下降（与假手术组比较,P＜0.05）;与模型组比较,小、高、中剂量组骨的最大载荷和挠度提高（P＜0.05）,高、中剂量组骨的弹性模量降低（与模型组比较,P＜0.05）,这种作用与尼尔雌醇作用相当（P＞0.05）;且补肾中药组方无明显的副作用.结论 补肾中药具有改善OVX大鼠骨生物力学的作用.     

不同频度PTHrP1-34对去卵巢大鼠骨密度和生物力学性能的影响

目的研究不同频度给予甲状旁腺激素相关肽（PTHrP1-34）对去卵巢大鼠腰椎和股骨骨密度（BMD）及生物力学性能的影响.方法将70只大鼠分为7组,即假手术对照组（Sham组）、去卵巢对照组（OVX组）、苯甲酸雌二醇治疗组（E组）、PTHrP1-34治疗组（包括P1D组、P2D组、P3D组、P1W组）.去卵巢4周后,后四组均给予PTHrP1-34 40μg/kg,分别按1次/d、1次/2d、1次/3d、1次/周皮下注射.治疗12周后处死大鼠,分离股骨、腰椎并测量其BMD,股骨做三点弯曲试验,第五腰椎做压缩试验.结果卵巢摘除术后,OVX组BMD较Sham组明显降低,表明大鼠骨质疏松模型建模成功.P1D组和P2D组股骨及腰椎BMD、最大载荷和弹性模量显著高于OVX组（P＜0.05）,与Sham组无统计学差异.结论PTHrP1-34 40μg/kg剂量,1次/d或1次/2d皮下注射,可以提高去卵巢大鼠的BMD,改善其生物力学特性,有效地防治骨质疏松.     

丹参素对卵巢去势骨质疏松大鼠TGF-β/Smad信号通路及骨密度的影响

目的 探讨丹参素对卵巢去势骨质疏松大鼠转化生长因子(TGF)-β/Smad信号通路及骨密度(BMD)的影响。方法 建立卵巢去势骨质疏松大鼠模型,随机分为模型组、丹参素低剂量组、丹参素中剂量组、丹参素高剂量组、雌激素组,每组12只,另取12只设为假手术组,分组药物处理后,以BMD仪测定大鼠股骨BMD,以骨科生物力学测试仪检测大鼠股骨生物力学状况,测定指标为弹性模量、最大载荷、屈服载荷;检测大鼠股骨骨矿物盐含量;以酶联免疫吸附试验(ELISA)检测大鼠血清核因子κB受体活化因子配体(RANKL)、骨保护素(OPG)水平;以蛋白免疫印迹法检测骨组织TGF-β/Smad通路蛋白表达情况。结果 与假手术组相比,模型组BMD、弹性模量、最大载荷、屈服载荷、骨矿物盐含量、血清OPG水平、骨组织TGF-β1表达、p-Smad3/Smad3降低,血清RANKL水平升高,差异有统计学意义(P<0.05)。与模型组相比,丹参素低、中、高剂量组和雌激素组BMD、弹性模量、最大载荷、屈服载荷、骨矿物盐含量、血清OPG水平、骨组织TGF-β1表达、p-Smad3/Smad3升高,血清RANKL水平降低,且丹...     

Interleukin-10 knockout mouse: a model for studying bone metabolism during intestinal inflammation

The objective was to compare bone mass (bone mineral content [BMC], bone mineral density [BMD]) and biomechanical strength of femurs and lumbar vertebrae from male and female wild-type (WT) and IL-10 knockout (KO) mice to determine if the IL-10 KO mouse model is appropriate for studying inflammation-associated bone abnormalities and potential interventions. Offspring from IL-10 KO and WT mice (n = 15 to 19 mice/gender per group) were studied until 13 weeks of age. IL-10 KO mice had a higher (P < 0.05) colonic histologic injury score and serum proinflammatory cytokines (IL-6, IL-1beta, TNF-alpha) than WT mice. IL-10 KO mice also experienced bone abnormalities as femur and verterbral BMC and BMD were lower (P < 0.05) compared with WT mice. Moreover, some biomechanical strength parameters such as femur yield load (P = 0.057) and resilience (P < 0.05) and peak load of lumbar vertebra 3 (P < 0.05) were lower than WT mice. Due to differences in body size, males had greater (P < 0.05) femur and vertebral bone mass as well as femur weight, length, yield load, resilience, and peak load than females. A significant interaction (genotype x gender) was only observed for femur resilience in which male WT mice had a greater (P = 0.009) resilience than all other groups. These results demonstrate that IL-10 KO mice develop bone abnormalities that accompany intestinal inflammation and elevated serum proinflammatory cytokines. Thus, the IL-10 KO mouse model may be useful for studying inflammation-associated bone abnormalities and potential therapeutic interventions.     

甲状旁腺素1-34对骨质疏松大鼠治疗作用的实验研究

目的探讨甲状旁腺素134(hPTH134)对骨质疏松的治疗作用以及与血钙、磷、维生素D代谢和生长因子的关系。方法用摘除大鼠双侧卵巢的方式制备骨质疏松模型(OVX),实验动物分为4个组:模型对照组(OVX组,摘除大鼠双侧卵巢不作任何处理);hPTH134治疗组(PTH组,摘除大鼠双侧卵巢12w后用hPTH134治疗8w);盐酸雷洛昔芬治疗组(摘除大鼠双侧卵巢12w后用雷洛昔芬治疗8w);假手术组(Sham组,仅切除卵巢周围的脂肪组织约3g,术后12w纳入实验)。应用HOLOGIC第4代双能X线4500W骨密度仪测大鼠腰椎、股骨上段骨密度值(BMD);以骨形态计量学测股骨骨小梁面积、矿化沉积率;用ELISA法测定血清IGF1水平和血清25OHVitD浓度以及血淋巴细胞VitD受体(VDR)含量。结果hPTH134治疗组、盐酸雷洛昔芬治疗组均较OVX组腰椎、股骨上段骨密度增高,组间比较差异有显著性(P<0.01)。hPTH134治疗组较盐酸雷洛昔芬治疗组股骨上段骨密度增高,两组之间差异有显著性(P<0.01)。hPTH134治疗组骨小梁面积明显增加、矿化沉积率增高。hPTH134治疗组、盐酸雷洛昔芬治疗组血清IGF1浓度值、血清25OHVitD浓度值升高,与OVX组比较差异有显著性(P<0.01)。各组血淋巴细胞VDR含量无明显变化,与OVX组比较差异无显著性(P>0.05)。结论hPTH134能够预防腰椎、股骨上段骨密度丢失,使骨小梁面积明显增加、矿化沉积率增高并且血清IGF1及血清25OHVitD浓度值升高,但对VDR含量无明显作用。     

Differential effects of isoflavones on bone formation in growing male and female mice

Few studies have examined the effects of isoflavones on bone formation during growth period in male and female animals. In this study, the effects of daidzein or genistein on bone formation were assessed in immature male and female mice. Five-week-old male and female mice were divided respectively into 3 groups (n = 8 per group) as follows: control group (C) fed a control diet (AIN-93G), daidzein group (D) fed a control diet containing 0.08% pure daidzein, and genistein group (G) fed a control diet containing 0.08% pure genistein. After 4 weeks, the male D group had a significantly higher bone mineral density (BMD) in whole body, lumbar spine, and femur than did the C group. On the contrary, BMD of the whole body and femur in the female D group was significantly lower than that in the C group. The BMD of the whole femur in the male G group also was significantly higher than for the C group. Histologic analysis revealed that the bone formation rate was significantly higher in the male D and G groups, and lower in the female D group compared with the C group. These results suggest that daidzein has a specific, sexually dimorphic effect on bone formation and BMD during growth period in mice.     

COPD患者骨密度与肿瘤坏死因子-α的关系

目的观察慢性阻塞性肺疾病患者骨密度的变化与血清肿瘤坏死因子-α（TNF-α）的相互关系。方法选取稳定期COPD患者32例,另选30例健康正常男性作为对照组,测定腰椎（L2-L4）、右髋骨（股骨颈、大转子、Word′s三角）的骨密度（BMD）,并测定骨代谢生化指标血钙（Ca）、磷（P）、碱性磷酸酶（AKP）、尿羟脯氨酸/尿肌酐（HOP/Cr）及TNF-α,分析骨密度与TNF-α的关系。结果 COPD患者与对照组相比,骨形成指标血钙（Ca）、磷（P）、血清碱性磷酸酶（AKP）无明显差异（P〉0.05）,而骨吸收指标尿羟脯氨酸/尿肌酐（HOP/Cr）明显增高,差异有显著性（P〈0.05）。COPD患者的腰椎与右髋骨（股骨颈、大转子、Word′s三角）的骨密度值较健康对照组均明显减低（P〈0.05）。COPD患者的TNF-α水平明显增高（P〈0.01）,TNF-α的水平与腰椎（L2-L4）及右髋骨（股骨颈、大转子、Word′s三角）的骨密度值均呈显著负相关（P〈0.01）。结论 COPD患者骨吸收增加,骨密度减低,继发于COPD的骨质疏松可能与慢性炎症和缺氧有关。     

Calcitonin versus Clodronate in the Prevention of Ovariectomy-Induced Osteopenia in Rats

The effects of salmon calcitonin and clodronate were compared in ovariectomised rats. Sixty female Wistar rats (∼260 g in weight) were fed the same diet and had the same living conditions. The rats were divided into the following groups: 15 rats with sham ovariectomy and no drug treatment (Sham-OVX); 45 rats with bilateral ovariectomy subdivided into 15 rats not receiving drug treatment (OVX group), 15 rats treated with subcutaneous salmon calcitonin, 2 U/kg/day every 2 days (OVX + CT group) and 15 rats treated with subcutaneous clodronate, 5 mg/kg/day every 2 days (OVX + Cl group). Sixty days after surgery, the rats were sacrificed and their femurs and fifth lumbar vertebrae were dissected and cleaned of soft tissue. Femur length, vertebral height, and bone mineral content and bone mineral density of the femur and fifth lumbar vertebra by dual-energy X-ray absorptiometry were measured. Calcitonin had a significant and stronger effect in preventing ovariectomy-induced osteopenia in the femur (OVX + CT vs OVX groups, p<0.0001); both calcitonin and clodronate had a significant effect on the fifth lumbar vertebra, which was greater in the calcitonin group (OVX + CT vs OVX + Cl groups, p<0.005). These findings indicate that calcitonin has a protective effect on both the axial (trabecular bone) and peripheral (cortical bone) skeletons, but clodronate only has a protective effect on the axial skeleton. Received: 28 May 1999 / Accepted: 29 July 1999     

Effects of raloxifene on bone mineral metabolism in postmenopausal Japanese women on hemodialysis

In addition to renal osteodystrophy, postmenopausal women on hemodialysis are at high risk for osteoporosis. Recent studies reported the effects of raloxifene, a selective estrogen receptor modulator for osteoporosis, in postmenopausal women. The present study evaluated the efficacy of raloxifene and its effects on bone mineral metabolism in postmenopausal Japanese patients on dialysis. In a prospective, multicentre study, 17 postmenopausal women on chronic hemodialysis with severe osteoporosis (bone mineral density [BMD]≤2 SD by bone densitometry) were treated with 60 mg/day raloxifene hydrochloride for 12 months. The study also included 10 age-matched control women. Vitamin D and calcium salts were not changed during the study. Intact parathyroid hormone (iPTH), serum calcium and phosphorus, and bone resorption marker (NTx) were measured, and BMD were determined by DEXA, at 0, 6, and 12 months after administration of raloxifene. The mean lumbar spine BMD at baseline was similar in the two groups. Raloxifene therapy (for 12 months) improved lumbar spine BMD (by 2.6%) in 53% of the patients, while 70% of the control group showed a reduction in BMD (by 4.0%). Raloxifene significantly decreased serum calcium and increased iPTH. Our results suggested that raloxifene improved trabecular BMD in postmenopausal Japanese women on hemodialysis. The effects of raloxifene on serum calcium and serum iPTH level suggest it improves bone resorption. Vitamin D and/or calcium salts should be added to raloxifene treatment to avoid secondary hyperparathyroidism.     

老年男性骨密度与年龄和性激素关系的研究

目的 探讨老年男性骨密度与年龄和性激素之间的关系.方法 双能X线吸收测定法(DEXA)测定360例老年男性腰椎正位(L1～4)、股骨颈、股骨大转子、ward's三角区和股骨干的骨密度,化学发光法测定血清总睾酮和雌二醇.根据年龄和骨密度进行分值,比较不同骨密度老年男性年龄和性激素的差异.结果 360例中共检出骨质疏松者48例,骨密度减少者72例,非骨质疏松者240例.老年男性股骨颈、股骨大转子、Ward's三角区、股骨干的骨密度随年龄的增长而下降(F值分别为3.038,3.029,3.024,3.021,P<0.05),年龄大于80岁组股骨颈、股骨大转子、Ward's三角区、股骨干骨密度分别为(0.701±0.140)、(0.682±0.185)、(0.629±0.211)、(0.986±0.160)g/cm2;年龄大于70岁组分别为(0.829±0.156)、(0.765±0.170)、(0.698±0.187)、(1.042±0.190)g/cm2;年龄大于60岁组分别为(0.875±0.138)、(0.800±0.130)、(0.731±0.145)、(1.071±0.125)g/cm2,但L1～4的骨密度差异无统计学意义(F=2.988,P>0.05).骨密度正常、骨密度减低和骨质疏松组血清总睾酮水平差异无统计学意义(F=3.032,P>0.05),而血清雌二醇水平在骨密度正常、骨密度减低和骨质疏松组分别为(180.6±62.3)、(130.5±39.9)、(110.5±68.5)ρmol/L,随骨密度减少,血清雌二醇水平降低,且差异有统计学意义(F=3.059,P<0.05).结论 老年男性骨密度随年龄增加而下降,雌激素水平可能影响老年男性骨质疏松的发生.     

雌激素联合甘草酸对去卵巢小鼠骨代谢及生物力学指标的影响

目的探讨雌激素联合甘草酸对去卵巢小鼠骨代谢及生物力学的影响。方法将60只小鼠随机分为6组,假手术组不作任何处理,余5组行去卵巢手术,术后4 d无异常开始灌胃给药。去卵巢组不予任何药物,雌激素组予32μg/（kg.d）雌激素,低剂量甘草酸组予50μg/（kg.d）甘草酸,高剂量甘草酸组予100μg/（kg.d）甘草酸,联合组予32μg/（kg.d）雌激素和50μg/（kg.d）甘草酸,给药45 d后处死小鼠。采用分析天平称量小鼠器官并计算内脏指数,检测血清钙及碱性磷酸酶（ALP）水平,化学比色法检测骨组织羟脯氨酸（Hyp）水平,计算股骨断裂载荷、最大载荷及第四腰椎最大载荷。结果低剂量甘草酸组子宫指数明显低于假手术组及雌激素组,Hyp高于去卵巢组;高剂量甘草酸组第四腰椎最大载荷明显低于雌激素组;联合组子宫指数、第四腰椎最大载荷明显高于去卵巢组,第四腰椎最大载荷明显高于假手术组;去卵巢组子宫指数低于假手术组,子宫指数、胸腺指数低于雌激素组;雌激素组子宫指数、第四腰椎最大载荷高于去卵巢组,胸腺指数低于去卵巢组,第四腰椎最大载荷高于假手术组;P〈0.05或0.01。结论甘草酸单用或联用雌激素对骨质疏松有一定的防治作用。     

降钙素治疗老年性骨质疏松症45例临床观察

目的　 观察降钙素对老年性骨质疏松症 (OP)的作用。　 方法 　将 80例OP病人随机分成 2组 ,降钙素组 45例 ,隔日肌注降钙素 5 0U ,每日加服碳酸钙 维生素D 0 6 ,对照组 35例 ,每日碳酸钙 维生素D 0 6 ,疗程均为 6月 ,每组用药 6月前后分别检测腰椎前后位、腰椎 3侧位右髋部骨密度 (BMD)、血清碱性磷酸酶 (ALP)、骨钙素(BGP)、酸性磷酸酶 (ACP)及尿吡啶酚 /肌酐 (Pyd/Cr)比值。　 结果 　降钙素组腰椎前后位 1～ 4及腰 3侧位BMD分别升高 11 8%、9 3 %、8 2 %、10 2 %和 10 3 % ,髋部BMD升高不明显 ,与此同时 ,血清ALP、BGP、ACP及Pyd/Cr比值明显降低 (P <0 0 5 ) ,疼痛改善。对照组用药物后无明显改变。　 结论 　小剂量降钙素能够增加老年性OP病人腰椎BMD ,其机理与抑制骨吸收 ,降低骨转换率有关。     

去卵巢大鼠血清乳铁蛋白与骨密度的相关性研究

目的 探讨去卵巢大鼠血清乳铁蛋白(LF)水平与骨密度(BMD)的关系,为临床应用乳铁蛋白提供实验依据.方法 采用4月龄Wistar雌性大鼠,随机分为假手术组(sham,20只)与去卵巢模型组(ovariectomy,OVX,40只,分为OVX I组与OVX Ⅱ组,各20只),分别于术后14与18周处死OVX I组与OV...