Effect of Ligustrazine on the Expression of LFA-1, ICAM-1 Following Bone Marrow Transplantation in Mice

Asablood activatingChinesedrug ,ligustrazinecanimprovethemicroenvironmentofbonemarrow ,increasetheadherentfunctionofstromalcellsandpromotethegrowthofhematopoieticcells[1] .Toin terprettherolesthatligustrazineplaysinbonemar rowhematopoieticreconstitutionan…     

Effects of Ligustrazine on Expression of Bone Marrow Heparan Sulfates in Syngeneic Bone Marrow Transplantation Mice

Hematopoietic reconstitution after bone marrowtransplantation (BMT ) is a complicated process,which concerns notonly with the quality and quanti-ty of hematopoietic stem cells(HSC) ,but also withthe recovery of hematopoietic inducing microenviron-ment (HIM) .The contactof HSC and HIM,whichregulates the proliferation,differentiation and self-maintenance of HSC,is critical for hematopoietic re-constitution.Heparan sulfates (HS) are a kind of gly-cosaminoglycans macromolecules composed of…     

Enhancing Effects of Ligustrazine on Expression of CD31 and Hematopoietic Reconstitution in Syngenic Bone Marrow Transplantation of Mice

Summary： The effect of ligustrazine on the expression of CD31 in syngenic bone marrow transplantation （BMT） mice was studied. Fifty-six Balb/c mice were divided into 3 groups： normal control group. BMT control group, and ligustrazine treated group. Syngenic BMT mouse models were established according to the literatures. In BMT control group and the ligustrazine treated group, the mice were given respecxively orally 0.2 mL saline and 2 mg ligustrazine twice a day. On the 7th, 14th, and 21st day after BMT, the mice were killed. The expression of CD31 on the surface of bone marrow nuclear cells （BMNC） was detected by flow cytometry. Peripheral blood leukocytes, platelets and BMNC were counted. Histological observation of bone marrow was made. The results showed thai in ligustrazine treated group the peripheral blood leukocylcs, platelets and BMNC counts, and the expression of CD31 on the day 7, 14, 21 after BMT were higher than in BMTcontrol group （P〈0.01 or P〈0.05）. In conclusion, ligustrazine could obviously enhance the CD31 expression on the surface of BMNC after syngcnic BMT in mice, which may be one of the mecha- nisms underlying the ligustrazine accelerating hematopoietic reconstitution in syngenic BMT.     

Expression of adherent molecule and cyclin by ligustrazine in bone marrow of mice with immune-induced aplastic anemia

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放射损伤小鼠骨髓VEGF、CFU-GM、CFU-F的变化及川芎嗪对其影响的研究

目的:探讨放射损伤小鼠骨髓血管内皮细胞生长因子(VEGF)、骨髓粒巨噬细胞集落形成单位(GFU-GM)数和骨髓成纤维细胞集落成单位(CFU-F)的变化意义及川芎嗪对其的影响。方法:健康昆明小鼠经6.0Gy60Coγ照射后,立即喂饲川芎嗪并设对照组和正常组,分别于第3、7、14、21天检测其骨髓VEGF、CFU-GM和CFU-F的变化。结果:照射后第3、7、14 d骨髓VEGF表达水平显著降低,但川芎嗪组高于对照组;随着时间推移骨髓VEGF表达水平逐渐恢复,照射后第21 d川芎嗪组已恢复正常(P>0.05),而照射组仍低于正常组。照射后骨髓CFU-GM和CFU-F明显受到抑制,但川芎嗪组显示了良好的保护作用。结论:放射损伤后小鼠骨髓VEGF的表达变化在造血功能的恢复过程中起重要作用;川芎嗪具有改善骨髓造血功能的作用。     

Study on the effect of ligustrazine on hematopoietic reconstitution in bone marrow transplantation mice

Hematopoietic reconstitution after bone marrowtransplantation is a complicated process CXCchemokines and their receptors play important rolesin this process. One of these receptors, CXCR4 expresses on hematopoietic stem cells, and is now takenas a "homing factor" in hematopoietic reconstitu--'tionll' 2]. Ligustrazine can protect the bone marrowmicroenvironment after irradiation or immune damage[', 4]. We established allogenic bone marrow transplantation mice models and explored the effects …     

Effects of Ligustrazine on Bone Marrow Microvessel Systemin the Early Period of Acute Radiation Injury in Mice

Ithasbeenprovedbymanystudlesthatthedifferent1ationanddevelopmentofthehematopoieticcellsdependonthesup-portandregulationofbonemarr0wmi-croenvironment,inwhichthevascularsys-temisanimp0rtantpart.Kn0speetalre-portedthatafterhigh-doseirradiationde-stroyedthehematopoieticcellsandthemi-crovesselsysteminbonemarrow,there-coveryofvascularsystemwasessentialforthehematopoiesisrehabilitatioin[l.2].There-fore,itisofgreatsignificancetostudytheeffects0fLigustrazine,akind0fs0called"blood-activatingandstasis-el…     

巴桑母酥油丸对放射线-化学复合损伤小鼠骨髓造血组织面积和基质细胞粘附力的影响

目的：研究藏药巴桑母酥油丸对放射线．化学复合损伤小鼠骨髓造血组织面积和基质细胞粘附能力的影响,探究桑母酥油丸促进其外周血像恢复的机制。方法：放射线一化学复合损伤小鼠灌胃巴桑母酥油丸14d后,制股骨切片,用图像分析软件测其骨髓造血组织面积百分比;进行骨髓基质细胞培养,测其对骨髓细胞黏附能力。结果：巴桑母酥油丸组的骨髓造血组织面积百分比显著高于空白组和生理盐水组;骨髓基质细胞培养第7、14、20d后对骨髓细胞的黏附力亦均显著高于空白组与生理盐水组。结论：促进骨髓造血组织恢复、提高基质细胞对造血细胞粘附力可能是巴桑母酥油丸经干预骨髓造血微环境进而促进放射线一化学复合损伤小鼠外周血像恢复的途径之一。,     

Effect of ligustrazine on hematopoiesis in bone marrow transplantation mice

Hematopoietic reconstitution after bone marrowtransplantation is a complicated process,whichconcerns with the quality and quantity ofhematopoietic stem cell (HSC) ,and also with therecovery of hematopoietic inducing microenvironment(HIM) .The contact of HSC and HIM is critical forhematopoietic reconstitution.1　MATERIALSAND METHODS1 .1　Materials1 .1 .1　 Animals　　 BALB/c mice of both sexes(clean class,close copulatory series (H- 2 d,MLSb) )were 8— 1 0 weeks old,and 1 7— 2 …     

白细胞介素12对急性放射病小鼠造血系统的影响

目的 研究重组鼠白细胞介素12(rmIL-12)对急性放射病小鼠造血系统的影响.方法 42只BALB/c小鼠均给予6.0Gy(60)γ射线全身照射,随机分为照射对照组、5和20μg/kg的rmIL-12治疗组,治疗组分别于照后1h及此后每3d一次分别腹腔注射5和20 μg/(kg·d)的rmIL-12,共5次,每日2...     

骨髓腔内移植对异基因骨髓移植小鼠造血的影响

目的:探讨骨髓腔内骨髓移植(Intrabonemarrowbonemarrowtransplantation,IBMBMT)对异基因骨髓移植小鼠造血功能的影响。方法:将供鼠C57BL/6(H2b)骨髓细胞经微量注射器直接注入到经致死量照射后的受鼠BalB/c(H2d)胫骨骨髓腔。在骨髓移植后1,7,14d分别检测受鼠外周血红细胞、白细胞、血小板;骨髓移植后7,14d分别检测受鼠骨髓中的CFUS。结果:IBMBMT组小鼠移植后7,14d的外周血红细胞、白细胞、血小板、骨髓脾集落形成单位(CFUS)均显著高于传统骨髓移植组(IVBMT)(P<0.01)。结论:IBMBMT较IVBMT更能促进异基因骨髓移植后造血功能重建。     

Study on the effects of Guiqi Oral Liquid in promoting recovery of hematopoiesis in acute irradiation injured mice

Objective: To explore the effects and possible mechanisms of Guiqi Oral Liquid (GQOL) on the recovery of hematopoiesis in acute irradiation injured mice.Methods: The acute irradiation injured mice were randomly divided into 2 groups: the treated group and the control group, and also a normal control group was set up with 6 mice in it receiving no treatment. After the mice in the former two groups were irradiated by 6.0 Gy⁶⁰Coγ-ray, every one of them was given 0. 4 ml GQOL or saline in equal volume through a gastric tube twice a day for 14 days. On the 4th, 8th and 14th day after irradiation, the bone marrow mononuclear cells (BMMNC) and megakaryocytes in bone marrow tissues of the mice were counted, the proportion of hematopoietic tissues (by area) was measured, and the expression of adhesion molecules, CD44 and CD54, in bone marrow were estimated by immunochemistry. The colony forming unit of spleen (CFU-S) in the mice were counted on the 8th day after irradiation.Results: On the 4th, 8th, 14th day after irradiation, the count of BMMNC and megakaryocyte, and the proportion of hematopoietic tissues in the treated group were higher than those in the control group (P<0.01 orP<0.05). CD44 and CD54 expression in the treated group were higher than those in the control group on the 4th and 8th day (P<0.01), but near normal on the 14th day (P<0.01). On the 8th day, CFU-S count in the treated group was higher than that in the control group (P<0.01).Conclusion: GQOL can regulate the expression of adhesion molecules, CD44 and CD54, in the bone marrow of the acute irradiation injured mice, which may be one of the mechanisms of GQOL in accelerating the early phase hematopoiesis recovery of mice. Supported by the National Natural Science Foundation of China (No. 39870926)     

川芎嗪对骨髓移植小鼠造血细胞SDF-1表达水平的影响

为探讨川芎嗪对骨髓移植(BMT)后造血重建过程中基质细胞来源因子(SDF-1)的作用,建立了典型同基因BMT小鼠模型,胃饲川芎嗪注射液每次2 mg,2次/d,分别于BMT后第7、14 d计数外周血细胞骨髓单核细胞(BMNC)并测定骨髓组织切片中SDF-1的表达.结果表明川芎嗪组BMT后第7、14 d外周血细胞计数、BMNC计数及骨髓组织切片中SDF-1表达水平均高于BMT组.提示川芎嗪能在骨髓移植造血重建早期促进骨髓基质细胞表达SDF-1,可能是促进BMT后造血干细胞(HSC)回髓、加速造血重建的机制之一.     

大鼠骨髓源内皮祖细胞分离和培养

目的:研究大鼠骨髓源内皮祖细胞(EPCs)分离、培养以及诱导向内皮细胞分化的方法。方法:采用密度梯度离心法从大鼠骨髓中分离单个核细胞,经贴壁后定向诱导培养2周。以CD34、CD133、VEGFR-2、vWF免疫细胞化学、荧光染色法以及Dil-acLDL结合FITC-UEA-1双荧光染色法鉴定EPCs。结果:贴壁细胞经培养诱导后3d开始伸展,4~7 d形成细胞集落,10~21 d增殖加速呈典型的"鹅卵石"样外观,并出现条索状结构且呈"微血管样"排列生长。细胞免疫荧光染色鉴定CD34、CD133、VEGFR-2、vWF阳性,Dil-acLDL联合FITC-UEA-1双染阳性。结论:从大鼠骨髓中分离单个核细胞,体外诱导培养后可以表现出部分内皮细胞的特征。     

Study on the Protective Effects of Compound Blood-activating Soup on Bone Marrow Hematopoietic Cells in Acute Radiation Injured Mice

ThisprojectwassupportedbyNationalScienceFoundationofChina(No.39670911)andTraditionalChineseMedicineSectionofthePublicHealthDepartmentofHubeiProvince.Ithasbeenknownthattheprominentmanifestationofbonemarrowintheearlyperiodofacuteradiationwasthathematopoieticcellsdecreasedrapidly,venoussinusexpanded,becominghyperemic,andbloodflowwasinstasisll].Onthebasisofthetheoryofblood-activatingandstasiseliminatingofChinesetraditionalmedicine,weusedablood--activatingandstasis--eliminatingChinesedrug,aco…     

蝎毒对放疗后小鼠骨髓造血功能保护作用的实验研究

目的探讨蝎毒对放疗后小鼠骨髓保护的效应及其机制。方法将小鼠分为对照、治疗和预防组,观察比较放疗后外周血WBC数、骨髓有核细胞数、脾重指数等造血功能指标。结果蝎毒多肽、蝎毒对放疗后小鼠外周血WBC均有明显促进作用,低剂量蝎毒多肽对放疗后小鼠外周血WBC及骨髓造血细胞具有明显促进作用;高剂量蝎毒多肽对放疗后小鼠外周血WBC具有明显促进作用。低剂量蝎毒对小鼠骨髓造血细胞具有明显促进作用。低剂量蝎毒多肽对小鼠外周血WBC及骨髓造血细胞促进作用最为明显。蝎毒多肽、蝎毒预防和治疗对放疗后小鼠脾重指数的恢复均有显著的促进作用。结论蝎毒多肽、蝎毒对放疗后小鼠骨髓造血功能具有保护作用。     

Effect of ligustrazine on expression of adherent molecule CD49d and Cyclin D2 in hematopoietic cells in acute radiation injured mice

Summary After irradiation by 8. 0 Gy γ-ray, each mouse was stomach-fed by 4 mg ligustrazine injection twice a day. On the 7th day after irradiation, CD₄₉d expression in ligustrazine-treated group was significantly higher than that in control group (P<0. 01), and showed no difference from that in normal group (P> 0. 05). On the 14th day after irradiation. CD₄₉d expression was increased in control group, but decreased significantly in ligustrazine-treated group (P<0. 01). The expression of Cyclin D₂ in spleen mononuclear cells (MNC) in ligustrazinetreated group was significantly higher than that in control group, but the ratio of G₀+G₁ phase cells was significantly lower in ligustrazine-treated group (P<0. 01). This finding indicated that ligustrazine could increase the expression of adherent molecule on bone marrow hematopoietic cells and Cyclin D₂ in spleen MNC, thereby promoting the growth of hematopoietic cells. This project was jointly supported by the National Foundation of Natural Sciences of China (No. 39670911) and Fund of Natural Sciences of Hubei Province (No. 96J080).     

小鼠脂肪源Flk1+CD31-CD34-细胞促造血恢复作用观察

目的:观察小鼠成体脂肪源Flk1 CD31-CD34-细胞体内外能否促进造血恢复.方法:用小鼠脂肪源Flk1 CD31-CD34-细胞作滋养层,接种造血细胞,观察培养后1～4周造血细胞数及粒-巨噬细胞系集落形成单位(CFU-GM)的变化,并设对照组.30只经137Cs全身照射0.35 Gy的雌性C57BL/6小鼠随机分为3组,空白对照组尾静脉注射生理盐水,对照组注射骨髓有核细胞,实验组注射骨髓有核细胞及脂肪源Flk1 CD31-CD34-细胞,注射后均检测外周血和骨髓有核细胞数及CFU-GM变化,PCR检测受体小鼠体内Y染色体的表达.结果:体外实验中实验组接种后2～4周造血细胞数均高于对照组(P均＜0.05),接种后1～4周CFU-GM值均高于对照组(P均＜0.05);体内实验中空白对照组小鼠移植后10 d左右死亡,实验组小鼠外周血和骨髓有核细胞数、CFU-GM从第2周起均高于对照组(P均＜0.05),第4周实验组小鼠骨髓细胞有Y染色体表达.结论:脂肪源Flk1 CD31-CD34-细胞能促进小鼠造血恢复.     

Effects of Platelet Factor 4 on Expression of Bone Marrow Heparan Sulfate in Syngenic Bone Marrow Transplantation Mice

Platelet factor 4 ( PF4) is a negativehematopoietic factor.It can inhibit the prolifera-tion of endothelial cells and hematopoietic stem/progenitor cells,particularly megakaryoryocyticcells,reversibly[1] ,inhibit DNA synthesis,blockcell cycle progression during S phase and reducethe sensibility of normal hematopoietic stem/pro-genitor cells,but not some cancer or leukemia celllines,to cytotoxic drugs and ionizing radia-tion[2 - 3] ,and it also can cause a population in-crease of the stem cel…     

升白胶囊对辐射所致小鼠白细胞减少症影响的研究

目的探讨升白胶囊对辐射所致小鼠外周血白细胞减少症的影响以及升高白细胞的作用机制。方法将60只小鼠随机分为5组,正常对照组、模型对照组、贞芪扶正胶囊组、升白胶囊大剂量组、升白胶囊小剂量组,每组12只。经低能X射线照射制备小鼠白细胞减少症模型,造模成功后各组分别给予相应药物灌胃。造模后第8d、第14d取血及股骨进行检测。观测小鼠外周血象、骨髓有核细胞数(BMNC)和骨髓DNA的含量。结果与正常对照组比较,模型对照组白细胞(WBC)数、BMNC数及骨髓DNA含量显著降低(P﹤0.01);与模型对照组比较,贞芪扶正胶囊组与升白胶囊大剂量组第8d、第14dWBC数及升白胶囊小剂量组第14dWBC数显著升高(P﹤0.01);与模型对照组比较,贞芪扶正胶囊组、升白胶囊大剂量组BMNC数及骨髓DNA含量显著升高(P﹤0.01或P﹤0.05),升白胶囊小剂量组骨髓DNA含量显著升高(P﹤0.05)。结论升白胶囊对低能X射线照射所致小鼠白细胞减少有显著升高作用,其机制与促进骨髓造血功能有关。