不同浓度的吗啡与5-氟尿嘧啶联合应用对MCF-7乳腺癌细胞凋亡与细胞周期的影响

目的研究吗啡与5-氟尿嘧啶(5-Fu)联合应用对MCF-7乳腺癌细胞增殖和细胞周期的影响。方法药物作用48 h后通过流式细胞技术(FCM)检测Annexin V-EGFP/PI染色的细胞早期凋亡和细胞周期情况。结果单独应用吗啡处理,早期凋亡率随着吗啡浓度的增加依次增加(P<0.05,P<0.01),浓度为250μM及1250μM时,早期凋亡率较未处理组明显增加(P<0.01)。单独应用500μM 5-Fu处理48 h,早期凋亡率亦增加(P<0.01)。吗啡与5-Fu联合用药时,早期凋亡率随着联合用药中吗啡浓度的增加依次增加(P<0.01)。各浓度吗啡与5-Fu联合应用,均具有协同作用(P<0.05,P<0.01)。单独应用吗啡处理时,G0/G1期细胞比例随着吗啡浓度的增加依次增加(P<0.01),S期细胞比例随着吗啡浓度的增加依次降低(P<0.01),而G2M期细胞比例无明显变化(P>0.05)。单独应用500μM 5-Fu处理时,G0/G1期细胞比例减少(P<0.01),S期细胞比例增加(P<0.01),G2M期细胞比例减少(P<0.05)。不同浓度的吗啡与5-Fu联合用药时,G0/G1期细胞比例随着联合用药中吗啡浓度的增加依次增加(P<0.05,P<0.01),较该浓度的吗啡单独处理组均减少(P<0.01);S期细胞比例随着吗啡浓度的增加依次降低(P<0.05,P<0.01),较该浓度的吗啡单独处理组均增加(P<0.01);G2M期细胞比例随着联合用药中吗啡浓度的增加依次降低(P<0.05,P<0.01),较该浓度的吗啡单独处理组均减少(P<0.01);各浓度吗啡与5-Fu联合应用,均具有协同作用(P<0.01)。结论一定浓度的吗啡、5-Fu及联合应用48小时能诱导MCF-7乳腺癌细胞早期凋亡,且与其中吗啡的浓度有一定的剂量依赖关系,两药联合应用具有协同作用。吗啡可以诱导MCF-7细胞主要被阻滞于G0/G1期,5-Fu可以使细胞循环周期主要被阻滞于S期,两药联合应用既抑制了G0/G1期到S期的转化,又抑制了S期向G2M期的转化,对细胞周期的影响也与其中吗啡的浓度有一定的剂量依赖关系     

葛根素对乳腺癌细胞MCF-7增殖及凋亡的影响

目的探讨葛根素对乳腺癌细胞株(MCF-7)细胞增殖及凋亡的影响。方法采用终浓度为0、20、50、100、200μg/ml葛根素处理对数生长期的MCF-7细胞,四甲基偶氮唑盐(MTT)法检测各浓度的增殖抑制率,Annexin V-FITC/PI双染流式细胞术及Hoechst染色检测细胞早晚期凋亡率及凋亡指数,碘化丙啶(PI)单染流式细胞术检测细胞周期,免疫印迹法检测凋亡相关蛋白Bcl-2、Bax和Cleaved caspase-3的表达。结果葛根素可提高MCF-7细胞的增殖抑制率,此效应呈现剂量和时间依赖性,且除24 h 20μg/ml外,其余浓度及作用时间的增殖抑制率均高于0μg/ml(P0.05);不同浓度葛根素处理48 h后的早期、晚期凋亡率、细胞凋亡指数、G0/G1期细胞比例、凋亡促进基因Bax和Cleaved caspase-3水平升高,而S期、G2/M期细胞比例、凋亡抑制基因Bcl-2水平降低(P0.05)。结论葛根素可抑制乳腺癌细胞MCF-7细胞增殖,促进其凋亡和细胞周期阻滞,该作用可能与提高凋亡促进蛋白表达、降低凋亡抑制蛋白表达有关。     

Fas途径调控芬太尼与5-氟尿嘧啶联合诱导MCF-7乳腺癌细胞凋亡

目的探讨芬太尼与5-氟尿嘧啶(5-FU)联合应用对人乳头癌细胞株(MCF-7)乳腺癌细胞凋亡的影响及Fas途径的调控机制。方法作用48 h后通过流式细胞技术(FCM)检测细胞凋亡检测试剂盒/碘化丙啶(Annexin V-EGFP/PI)染色的细胞凋亡情况,通过Western印迹检测Fas、半胱氨酸蛋白酶(Caspase)-8表达情况。结果 1μmol/L及10μmol/L浓度的芬太尼单独应用均引起凋亡率增加,Fas、Caspase-8表达增强(P0.05,P0.01);5-FU单独应用后凋亡率亦明显增加,Fas、Caspase-8表达增强(P0.01);三种浓度芬太尼与5-FU联合应用均具有协同作用(P0.01)。Caspase-8与Fas表达变化呈显著正相关(r=0.877,P0.01)。结论一定浓度的芬太尼、5-FU及联合应用48 h对MCF-7乳腺癌细胞有促进凋亡作用,并显著上调Fas表达和Caspase-8活性,两药联合应用启动外源性凋亡途径具有协同作用。     

洛铂联合伊班膦酸对人乳腺癌细胞MCF-7增殖的影响

目的研究洛铂联合伊班膦酸对人乳腺癌MCF-7细胞体外培养增殖的抑制作用。方法采用MTT法检测单独应用洛铂、伊班膦酸及洛铂联合伊班膦酸对MCF-7细胞的抑制率。应用流式细胞仪检测不同药物作用下,细胞凋亡率及细胞周期变化。结果各给药组均对MCF-7细胞有抑制作用,且随着时间延长而增强(P<0.01)。联合用药组较单独用药组抑制作用更强(P<0.05)。流式细胞仪检测各给药组凋亡率均升高,较空白对照组差异有统计学意义(P<0.01)。各给药组G0/G1期比例升高,S及G2/M期比例下降,与空白对照组相差异均有统计学意义(P<0.01)。结论洛铂与伊班膦酸均能抑制体外培养的乳腺癌MCF-7细胞。洛铂与伊班膦酸联合应用具有协同作用。     

芬太尼与5-氟尿嘧啶联合诱导MCF-7乳腺癌细胞凋亡及其机制

目的研究芬太尼与5-氟尿嘧啶(FU)联合应用对MCF-7乳腺癌细胞凋亡的影响及内、外源性途径的调控机制。方法作用48 h后通过流式细胞技术(FCM)检测Annexin V-EGFP/PI染色的细胞凋亡情况,通过免疫细胞化学检测Bcl-2、Bax、Fas表达情况。结果单独应用芬太尼时1μmol/L与10μmol/L浓度组凋亡率均增加(P0.05,P0.01),Bcl-2表达减弱(P0.05,P0.01)、Bax表达增强(P0.01)、Fas表达增强(P0.01);单独应用500μmol/L 5-FU后凋亡率亦明显增加(P0.01),Bcl-2表达减弱(P0.01)、Bax、Fas表达增强(P0.01);联合应用芬太尼与5-FU,协同作用在3组均显现(P0.05,P0.01)。结论芬太尼、5-FU在一定浓度范围内单独或联合应用可促进MCF-7乳腺癌细胞凋亡,并使Bcl-2表达下调,Bax、Fas表达上调。联合应用两类药物可能在内源性、外源性途径调控细胞凋亡上显示协同效应。     

内源性途径调控芬太尼与5-氟尿嘧啶联合诱导的MCF-7乳腺癌细胞凋亡

目的探讨芬太尼与5-氟尿嘧啶(FU)联合应用对MCF-7乳腺癌细胞凋亡的影响及内源性途径的调控机制。方法作用48 h后通过流式细胞技术检测亚二倍体峰细胞凋亡情况,通过Western印迹检测Bcl-2、Bax表达情况。结果 1μmol/L及10μmol/L浓度的芬太尼单独应用均引起凋亡率增加(P0.01),Bcl-2表达减弱(P0.01),Bax表达增强(P0.05,P0.01);5-FU单独应用后凋亡率亦明显增加(P0.01),Bcl-2表达减弱(P0.01),Bax表达增强(P0.01);三种浓度芬太尼与5-FU联合应用均具有协同作用(P0.05,P0.01)。结论一定浓度的芬太尼、5-FU及联合应用48h对MCF-7乳腺癌细胞有促进凋亡作用,并显著下调Bcl-2表达、上调Bax表达,两药联合应用启动内源性凋亡途径具有协同作用。     

丹皮酚联合5-FU对食管癌EC9706细胞增殖及凋亡的影响

目的:探讨丹皮酚(paeonol,Pae)单独及联合5-Fu对人食管癌EC9706细胞的增殖抑制及凋亡诱导作用.方法:采用6种浓度的Pae(7.81、15.63、31.25、62.50、125.00、250.00 mg/L)、3种浓度的5-FU(12.50、25.00、50.00 mg/L)及Pae(31.25 mg/L)和5-FU(12.50 mg/L)联合分别处理EC9706细胞24、48、72 h.同时设对照组(细胞不做处理),采用MTT法检测各个时间段细胞的增殖情况:采用流式细胞术检测4种浓度的Pae(31-25、62.50、125.00、250.00 mg/L)处理EC9706细胞72 h后细胞周期的变化:倒置显微镜下观察各Pae组细胞各时间段形态学变化,HE染色光镜下观察凋亡细胞:采用免疫细胞化学法检测经Pae(31.25 mg/L)、5-FU(12.50mg/L)单独和联合作用48 h后细胞中凋亡相关蛋白Bcl-2及Bax的表达.结果:Pae、5-FU可明显抑制EC9706细胞增殖,并随着浓度的增加和作用时间的延长而增强(P<0.05),Pae与5-FU联合用药比单用Pae或5.FU抑制效果更明显(P<0.05);Pae作用后EC9706细胞中G0/G1期和G2/M期细胞比例下降、S期细胞比例上升(Pae 125.00 mg/L组:G0/G1期21.18%±2.28% vs 62.17%±5.23%、G2/M期0.76%±0.54% vs 9.92%±3.10%、S期78.06%±2.82% vs 27.91%±2.13%,均P<0.05):HE染色光镜下可见典型的肿瘤细胞凋亡改变:Pae、5-FU可下调EC9706细胞中Bcl-2蛋白表达,同时增强EC9706细胞中Bax蛋白的表达,联合用药组较单药组作用更为明显(2.21±0.14 vs 5.67±0.30,4.22±0.34;8.55±0.33 vs 3.90±0.27,6.28±0.26,均P<0.05).结论:Pae可明显抑制人食管癌EC9706细胞的增殖.促进其凋亡,Pae联合5-FU作用更为明显.     

姜黄素对乳腺癌MCF-7、MDA-MB-231细胞增殖、侵袭的影响及其机制探讨

目的观察姜黄素对雌激素受体阳性（ER＋）乳腺癌细胞MCF-7和ER-乳腺癌细胞MDA-MB-231增殖、侵袭的影响,并探讨其机制。方法将MCF-7/MDA-MB-231随机分为四组,姜黄素高、中、低剂量组（姜黄素Ⅰ、Ⅱ、Ⅲ组）分别加入5、10、20μg/ml的姜黄素,对照组不加。MTT法检测细胞的增殖能力,流式细胞仪分析细胞周期,通过Boyden小室观察细胞侵袭力,RT-PCR法检测细胞中的MMP-9 mRNA。结果与对照组相比,姜黄素各剂量组细胞增殖下降,G0/G1期及S期细胞比例减少,而G2/M期细胞比例显著增加,细胞侵袭指数降低,细胞中的MMP-9 mRNA表达下降,P均〈0.05。结论姜黄素能抑制MCF-7/MDA-MB-231细胞的增殖、侵袭作用,与其降低MMP-9基因表达有关。     

5-氟尿嘧啶联合热疗体外诱导Hep-2细胞凋亡及细胞周期阻滞

目的探讨5-氟尿嘧啶(5-FU)联合热疗对人喉癌细胞株Hep-2细胞增殖抑制率、细胞凋亡率及细胞周期进程的影响。方法应用噻唑蓝(MTT)染色法检测不同条件下Hep-2细胞的增殖抑制率,流式细胞仪检测细胞周期进程的变化及凋亡率。结果单纯热疗组、单纯5-FU组及联合组对Hep-2细胞的增殖抑制率分别为13.7%、22.3%和46.5%,与对照组相比差异显著(P<0.01)。流式细胞仪结果显示,G0/G1期细胞增多,S期细胞减少,G2/M期细胞相对增多,细胞凋亡率升高,组间比较差异显著。结论 5-FU联合热疗能显著提高Hep-2细胞的增殖抑制率,抑制Hep-2细胞G1期向S期转化进程,诱导Hep-2细胞凋亡。     

中药复方“益肾固冲汤”对人乳腺癌细胞株MCF-7增殖及凋亡的影响

目的探讨益肾固冲汤抑制乳腺癌细胞增殖,促进其凋亡的作用和抗癌分子的机制。方法体外培养人乳腺细胞株(MCF)-7细胞利用MTT法观察益肾固冲汤对人乳腺癌MCF-7细胞增殖的抑制作用,用流式细胞仪检测益肾固冲汤对人乳腺癌MCF-7凋亡及细胞周期的影响。结果益肾固冲汤对人乳腺癌MCF-7细胞增殖具有抑制作用,都具有明显的时-效和量-效关系。人乳腺癌细胞MCF-7细胞经过不同浓度的益肾固冲汤处理24、48、72 h后,各组均呈现不同程度的凋亡,且凋亡率随着计量的增加、时间的延长而逐渐增加。益肾固冲汤在40μg/ml量、作用72 h时具有统计学差异,细胞凋亡率最高,达17.93%,与正常对照组相比,G0/G1期细胞比率随作用时间延长在逐渐减少,G2/M期的细胞比率随作用时间延长在逐渐增加,细胞生长周期较明显停滞在G2/M期,具有明显时间依赖性。结论益肾固冲汤可以抑制人乳腺癌MCF-7细胞的增殖,诱导细胞凋亡,具有明显的量效和时效关系。细胞的增殖抑制与诱导凋亡作用与G2/M期有关。     

青少年高血压的研究进展

随着人们生活和行为方式的改变,高血压发病明显呈年轻化趋势。在青少年时期识别高血压病高危人群有助于早期进行有效干预和治疗,降低未来高血压的发生率及其严重性。现试从青少年高血压的诊断、发病因素、特点、治疗策略等方面的研究进展作一综述。     

Morbidity in cardiovascular diseases in immigrants in Sweden

INTRODUCTION: Although immigration to Sweden has increased in the last few decades, the incidence rates of cardiovascular disease and coronary heart disease in immigrants are unknown. The aim of the present study is to estimate whether place of birth affects the incidence rates of cardiovascular disease and coronary heart disease. MATERIAL AND METHODS: The study was designed as a follow-up study on morbidity in cardiovascular disease and coronary heart disease between 1 January 1997 and 31 December 1998, including three and a half million persons with age range 35-64 years, of whom 550 000 were born abroad, from the database MigMed consisting of the whole Swedish population. Incidence rates and relative risks were estimated by indirect standardization and a proportional hazard model. RESULTS: The age-adjusted risk of coronary heart disease was higher in most foreign-born groups than in Swedes. For example, in nine of 12 male groups, the relative risks varied between 1.1 and 2.2, and in seven of 12 female groups, the relative risks varied between 1.4 and 2.5. When also adjusting for level of education and employment status, the risks were still high, but on a lower level. CONCLUSIONS: Foreign-born people possess an over-risk of cardiovascular or coronary heart disease(CVD/CHD) compared with Swedish-born persons, also when level of education and employment status are taken into account.     

Pulmonary sporotrichosis in Oklahoma in susceptibilities in vitro

Six cases of pulmonary sporotichosis were observed in 2 institutions in Oklahoma City, Okla. Three of the patients were treated with iodides with or without surgery. Although one patient required a second course of iodides, the patients have remained well after at least 34 months of follow-up. Three patients treated with amphotericin B, single course as well as multiple courses, and other antifungal agents (hydroxystilbamidine and miconazole) have all relapsed. These cases and a reviewed of more than 40 cases of pulmonary sporotrichosis susceptibilities of Sporothrix schenckii that we observed in vitro suggest that amphotericin B is not an effective agent for the treatment of pulmonary sporotrichosis. It is our opinion that the treatment of choice for pulmonary sporotrichosis is a supersaturated solution of potassium iodide. If the patient is allergic to the medication or fails to respond, then a combination of amphotericin B plus flucytosine may be tried.     

C·肉毒杀鼠素(冻干剂)应用研究

目的为研究C·肉毒杀鼠索对杀灭达乌尔黄鼠(简称黄鼠)的大面积应用情况和对家畜、家禽的毒害作用,进行了C·肉毒杀鼠素的应用研究.方法大面积投毒采用ES-2药饵撒播机[1],间隔约80m进行条投.羊、鸡采用直接灌胃.结果大面积应用的灭鼠率为83.72%.对羊、鸡最高剂量分别为500万MLD、150万MLD,均未出现中毒现象.结论 C·肉毒杀鼠素是较为理想的草原大面积杀灭黄鼠的理想、首选药物.     

Differences in outcome in primary angioplasty in women.

BACKGROUND: In acute myocardial infarction (AMI), primary percutaneous transluminal coronary angioplasty (PTCA) has proved to be the best therapeutic approach. Several factors have been associated with worse outcome in AMI in females. Are there differences in outcome in women undergoing PTCA for AMI? AIM: To evaluate gender influence on clinical outcome and in-hospital mortality in patients with AMI who undergo primary percutaneous interventions. METHODS: We studied 245 consecutive patients (72 women, 29.4 %), who underwent primary PTCA between January 2000 and December 2001. The following parameters were analyzed: risk factors for coronary artery disease including hypertension, diabetes, smoking, hypercholesterolemia and family history, previous AMI, PTCA or angina, pain-to-balloon time, extent of coronary disease and outcome. RESULTS: Female patients were older (67.9+/-11.6 vs. 59.6+/-13; p < 0.001) with a higher prevalence of hypertension (65.3 % vs. 47.4 %; p < 0.05) and angina (29.0 % vs. 16.0 %; p < 0.05) and lower prevalence of smoking (27.8 % vs. 54.3 %; p < 0.001). Pain-to-balloon time was longer in women (6.8+/-4.1 vs. 5.4+/-3.7 hours; p < 0.05). Extent of coronary disease was similar in both groups. Glycoprotein IIb/IIIa inhibitors were used in 84.7 % of women and 90.8 % of men. The frequency of hemorrhagic complications (5.6 % vs. 5.2 %) and arrhythmias (15.3 % vs. 10.4%) and in-hospital mortality (9.7 6.4 %) were higher in females, although without statistical significance (p = NS). Hospitalization time was similar in both groups. CONCLUSIONS: Despite the growing awareness of a gender bias in therapeutic approaches to AMI, there are still some differences in outcome, with a trend towards higher mortality rates in women. Older age and longer pain-to-balloon time could account for this.