过氧化物酶抗体在妊娠甲状腺功能紊乱发生中的诊断价值

目的探讨血清甲状腺过氧化物酶抗体(TPOAb)在筛查孕妇发生自身免疫性甲状腺功能紊乱中的诊断价值。方法选取确诊甲状腺功能紊乱的孕妇75例,非甲状腺功能紊乱孕妇145例,采用微粒子酶免疫分析法(MEIA)和化学发光微粒子免疫分析法(CMIA)分别测定血清甲状腺过氧化物酶抗体(TPOAb)水平和促甲状腺激素(TSH)水平。结果(1)甲状腺功能紊乱组血清TSH和TPOAb水平与非紊乱组比较,差异均有统计学意义(P<0.01);(2)甲状腺功能紊乱组中TPOAb呈较高的阳性率(84.0%),非紊乱组孕妇TPOAb阳性率达11.0%;(3)TSH异常组中约半数孕妇(53.3%)呈TPOAb阳性。TSH正常组中有14.5%孕妇TPOAb呈阳性反应;(4)TPOAb阳性组中有65.6%的孕妇出现TSH紊乱;(5)较高TSH水平(>4.67mIU/L)组中,TPOAb阳性率较高(55.7%),低TSH水平组(<0.47mIU/L)中TPOAb阳性率则较低(9.9%);(6)各组高龄孕妇与育龄孕妇组之间血清TSH和TPOAb水平差异无统计学意义(P>0.05)。结论TPOAb可作为孕妇产前监测指标,有助于临床早期诊断并明确妊娠期甲状腺功能紊乱的病因。从诊断价值上看,TSH并不能完全取代TPOAb检测。TPOAb异常可能预示孕妇发生自身免疫性甲状腺疾病的可能和风险。     

215例妊娠早期甲状腺过氧化物酶抗体和促甲状腺激素检测结果分析

目的探讨孕妇妊娠早期甲状腺过氧化物酶抗体(TPOAb)和促甲状腺激素(TSH)检测的意义。方法选择215例孕周在16~24周的孕妇,采用电化学发光免疫分析法检测外周血血清TPOAb和免疫放射法检测TSH的分泌水平。结果 215例孕妇中,98例TSH异常的孕妇TPOAb阳性率为72.4%;117例TSH正常的孕妇中TPOAb阳性率为9.4%,TSH异常的孕妇TPOAb阳性发生率明显高于TSH正常的孕妇,差异有统计学意义(P0.05)。结论 TPOAb和TSH检测可以提高妊娠合并甲状腺功能紊乱的诊断,对于保证胎儿正常发育和孕妇健康具有重要意义。     

甲状腺过氧化物酶抗体在孕期筛查中的临床价值

目的：血清甲状腺过氧化物酶抗体（TPOAb）检测在筛查孕期甲状腺功能中的临床应用价值。方法收集2014年12月～2015年8月间本院妊娠中期孕妇血清204例为观察组，观察组依据促甲状腺激素（TSH ）、游离三碘甲状原氨酸（FT3）以及游离甲状腺素（FT4）水平分为二组，其中三项指标有超出参考范围者归为甲状腺功能紊乱组（89例），三项指标均正常者归为非甲状腺功能紊乱组（115例），同时收集健康体检女性血清380例作为对照组。利用发光免疫法检测观察组和对照组的血清T PO-Ab水平，依次分析TSH、FT3、FT4不同水平对TPOAb阳性率的影响。结果 TSH、FT3、FT4任一指标水平异常均会对 TPO-Ab阳性率造成影响，因此孕妇甲状腺功能紊乱组、甲状腺功能非紊乱组和对照组任意两组间的 TPOAb阳性率均具有显著性差异（P＜0．05）。结论本文显示，甲状腺功能紊乱与非紊乱组孕妇的TPOAb阳性率均高于健康对照组，甲状腺功能紊乱组又高于甲状腺功能非紊乱组，表明孕期自身免疫性抗体TPOAb阳性率可能增高而影响甲状腺功能，建议孕期初筛TPOAb。     

妊娠妇女甲状腺自身抗体与甲状腺功能的相关性分析

[目的]探讨沧州地区妊娠妇女血清抗甲状腺过氧化物酶抗体(TPOAb)、甲状腺球蛋白抗体(TGAb)的阳性率与促甲状腺素(TSH)水平的关系.[方法]选取妊娠妇女786例,按孕周分为T1、T2、T3期三组,采用电化学免疫分析法测定其血清TSH及TPOAb、TGAb水平.根据TSH水平分为TSH异常组和TSH正常组;根据TPOAb、TGAb水平分为TPOAb和TGAb阳性组(双阳性)、TPOAb阳性组、TGAb阳性组、TPOAb和TGAb阴性组(双阴性).[结果]妊娠妇女在T1、T2、T3期TPOAb阳性率分别为:9.95％、11.07％、11.22％,TGAb的阳性率分别为:8.14％、9.09％0、8.65％;TSH异常组TPOAb、TGAb阳性率升高,与TSH正常组比较,差异均具有统计学意义(P＜0.05);双阳性组TSH水平升高,且与TPOAb阳性组、TGAb阳性组、双阴性组比较,差距均具统计学意义(P＜0.05).[结论]TSH异常组TPOAb和TGAb阳性率高,TPOAb、TGAb均阳性者TSH水平升高,TPOAb、TGAb和TSH水平均可作为评估妊娠期妇女甲状腺功能的指标.     

TPOAb阳性对妊娠期妇女甲状腺功能及不良妊娠的影响

目的探讨甲状腺过氧化物酶抗体(TPOAb)阳性对妊娠期妇女甲状腺功能及不良妊娠的影响。方法选取在该院接受产前检查的106例单胎孕妇,其中53例TPOAb阳性的孕妇为观察组,53例TPOAb阴性的孕妇为对照组。对比两组孕妇孕晚期甲状腺功能异常的发生率;同时对比两组孕妇不良妊娠事件和新生儿并发症的发生率。结果观察组单纯TSH升高的发生率为11.32%,甲状腺功能异常的发生率为26.42%,均显著高于对照组(均P0.05);观察组不良妊娠事件发生率为32.08%,显著高于对照组(P0.05);观察组吸入综合征发生率为15.09%,新生儿并发症发生率为24.53%,均显著高于对照组(均P0.05)。结论妊娠早期TPOAb阳性的孕妇,妊娠晚期甲状腺功能异常的发生率显著增加,同时不良妊娠事件和新生儿并发症的发生风险也显著上升。临床应重视妊娠早期TPOAb的筛查。     

妊娠期甲状腺功能指标筛查的临床分析

目的分析正常妊娠早中晚期孕妇甲状腺功能指标筛查的结果 ,了解本地区孕妇甲状腺功能异常患病率。方法选取来我院孕检的孕妇3638例,分为早期妊娠妇女498例、中期妊娠妇女2412例和晚期妊娠妇女728例,不同孕期再按年龄分为≤30岁组和30岁组,采用电化学发光法对各组妇女的血清促甲状腺素(thyrotropin,TSH)、游离三碘甲状腺氨酸(free triiodothyronine,FT3)、游离甲状腺素(free thyroxine,FT4)和甲状腺过氧化物酶抗体(thyroid peroxidase antibody,TPOAb)4项甲状腺功能指标进行检测,并对检测结果进行统计学分析。结果 FT3、FT4随着孕期的延长逐渐降低,TSH和TPOAb随着孕期延长逐渐升高,且各项指标妊娠各期≤30岁组和30岁组差异均有统计学意义(P均0.05)。30岁以上孕妇甲状腺功能异常率为43.69%,30岁以下孕妇甲状腺功能异常率为20.74%。30岁组患者甲减、亚临床甲减、低FT4血症、甲亢、亚临床甲亢的阳性率均高于≤30岁组患者,而单纯TPOAb阳性低于≤30岁组,且差异均有统计学意义(P均0.05)。结论不同孕期不同年龄段的甲状腺功能指标存在差异,应加强本地区不同孕期孕妇甲状腺功能指标正常参考范围的研究。低风险孕妇仍有较高的甲状腺功能异常率,建议全面开展孕期甲状腺功能检测,及时发现和治疗甲状腺疾病。     

甲状腺自身抗体与妊娠期甲状腺功能异常的相关性研究

目的探讨甲状腺自身抗体与妊娠期甲状腺功能异常的相关性。方法选择2016年3月至2017年10月在首都医科大学附属北京友谊医院妇产科门诊首次就诊并建立围产保健档案的妊娠妇女共4 399例进行前瞻性研究。于妊娠第8~10周行甲状腺过氧化物酶抗体(TPOAb)、甲状腺球蛋白抗体(TGAb)和甲状腺功能(TSH、FT4)的测定;于妊娠第24~26周、妊娠第36~37周行甲状腺功能(TSH、FT4)的测定。根据妊娠期特异性参考值范围对妊娠期甲状腺功能异常进行诊断。计算早孕期妇女甲状腺自身抗体的阳性率,并分析甲状腺自身抗体阳性对妊娠期甲状腺功能异常发生率的影响。结果纳入的4 399例研究对象中,TPOAb阳性率为12. 0%(529/4 399); TGAb阳性率为9. 1%(399/4 399); TPOAb和TGAb均阳性孕妇占总人数的5. 0%(220/4 399)。TPOAb阳性和TGAb阳性孕妇妊娠期临床、亚临床甲状腺功能减退症和低T4血症的发生率分别为3. 0%、9. 3%、3. 8%和5. 0%、11. 0%、3. 5%; TPOAb和TGAb均阳性妇女中上述三种甲状腺功能异常的发生率分别为6. 8%、10. 9%、4. 5%。三组与TPOAb和TGAb抗体均阴性者比较差异均具有统计学意义(P 0. 05)。TPOAb阳性(OR值分别为4. 79、12. 9、1. 91,P 0. 05)、TGAb阳性(OR值分别为5. 76、38. 4、1. 77,P 0. 05)、TPOAb和TGAb均阳性(OR值分别为5. 07、31. 1、2. 4,P 0. 05)均显著增加妊娠期临床、亚临床甲状腺功能减退症、低T4血症的发生风险。TPOAb阳性、TGAb阳性、TPOAb和TGAb均阳性组与TPOAb和TGAb抗体均阴性者比较妊娠期临床、亚临床甲状腺功能亢进症、高T4血症的发生率差异均无统计学意义(P 0. 05)。结论甲状腺自身抗体阳性妇女妊娠期发生甲状腺功能异常的风险明显增加。对甲状腺自身抗体阳性妇女加强甲状腺功能筛查对诊断和治疗妊娠期甲状腺功能异常有重要价值。     

孕中期TPO抗体、TSH和新生儿甲状腺功能关系探讨

选择699例妊娠中期孕妇作为研究对象，根据TPOAb结果分为阳性和阴性两组，比较两组孕妇甲状腺功能以及产后新生儿TSH指标。在TPOAb阳性和阴性孕妇中，TSH水平差异有显著性（P＜0.05）；TPOAb阴性组产妇的新生儿TSH浓度低于TPOAb阳性组（P＜0.05）。孕妇TPOAb阳性可能会对新生儿甲状腺功能造成一定影响。     

孕早期亚临床甲状腺功能异常孕妇的甲状腺超声特征及妊娠结局分析

目的:分析孕早期亚临床甲状腺功能异常孕妇的甲状腺超声特征及其对妊娠结局的影响。方法:选取68例孕早期亚临床甲状腺功能异常孕妇,以46例亚临床甲状腺功能亢进为A组,22例亚临床甲状腺功能减退为B组。分析两组孕妇的甲状腺超声特征和甲状腺过氧化物酶抗体(TPOAb)升高孕妇的甲状腺超声特征;对比两组孕妇妊娠结局及新生儿不良妊娠事件的发生率。结果:A组TSH降低孕妇的甲状腺实质回声均无明显异常,4例腺体血流信号轻度增多,而伴有TPOAb升高的孕妇中,4例出现甲状腺实质回声轻度弥漫性改变以及6例腺体血流信号不同程度增多。B组4例TSH升高孕妇的甲状腺实质回声轻度弥漫性改变,2例腺体血流信号轻度增多,而伴有TPOAb升高的孕妇中,9例出现甲状腺实质回声不同程度的弥漫性改变,6例腺体血流信号不同程度增多。TPOAb≥1300IU/L孕妇甲状腺实质回声异常率为100.00%,高于TPOAb1300IU/L孕妇(P0.05);TPOAb≥1300IU/L和TPOAb1300IU/L孕妇甲状腺腺体血流增多发生率的对比,差异没有统计学意义(P0.05)。两组孕妇妊娠结局和新生儿不良妊娠事件的对比,差异没有统计学意义(P0.05)。结论:孕早期亚临床甲状腺功能异常,尤其是高水平TPOAb可引起异常甲状腺超声表现。亚临床甲状腺功能亢进与亚临床甲状腺功能减退对妊娠结局的影响可能无显著的差异。     

深圳市松岗街道5505例孕妇甲状腺功能调查分析

目的 调查该地区孕妇甲状腺功能状况并进行分析.方法 将2018年2月至2020年1月于该院接受常规孕检的5505例孕妇纳入研究.根据孕期将纳入研究的孕妇分为早期妊娠组3101例、中期妊娠组2278例、晚期妊娠组126例.对3组的甲状腺功能异常情况、甲状腺激素水平、抗甲状腺球蛋白抗体(A-TG)以及抗甲状腺过氧化物酶抗体(TPOAb)阳性率进行比较.此外,分析孕妇甲状腺功能与胎儿不良妊娠结局的关系.结果 中期妊娠组甲状腺功能异常率高于早期妊娠组和晚期妊娠组(P<0.05).早期妊娠组、中期妊娠组、晚期妊娠组促甲状腺激素(TSH)水平依次升高,而游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)水平依次降低,组间比较差异均有统计学意义(P<0.05).早期妊娠组、中期妊娠组、晚期妊娠组A-TG阳性率依次降低,而TPOAb阳性率依次升高,组间比较差异均有统计学意义(P<0.05).甲状腺功能异常孕妇不良妊娠结局总发生率明显高于甲状腺功能正常孕妇(P<0.05).结论 该地区孕妇甲状腺功能异常发生率较高.     

Prevalence of abnormal thyroid stimulating hormone and thyroid peroxidase antibody-positive results in a population of pregnant women in the Samara region of the Russian Federation.

Undiagnosed thyroid disease is a common problem with significant public health implications. This is especially true during pregnancy, when the health of both the mother and the developing child can be adversely affected by abnormal maternal thyroid function. Measurement of serum thyroid stimulating hormone (TSH) and thyroid peroxidase antibodies (TPO-Ab) are two common ways to assess maternal thyroid status. The objective of our study was to determine the prevalence of abnormal TSH and TPO-Ab tests in a population of pregnant women in the Samara region of the Russian Federation. Serum samples were obtained from 1588 pregnant women as part of their routine antenatal care. TSH and TPO-Ab were measured, and trimester-specific reference values for TSH (2.5-97.5 percentiles) were calculated using TPO-Ab-negative women. TSH results outside these ranges were considered abnormal; TPO-Ab levels outside the manufacturer's reference range (>12 IU/mL) were considered abnormal. Overall, the prevalence of abnormal results was 6.3% for TSH and 10.7% for TPO-Ab. High TSH (>97.5 trimester-specific percentile) and TPO-Ab-positive results were most common in the first trimester (5.7% and 13.8%, respectively). TSH levels were associated with gestational age and TPO-Ab status, and with maternal age in TPO-Ab-negative women. TPO-Ab status was associated with both maternal and gestational age. Women with TSH >2.5 mIU/L had a significantly increased risk of being TPO-Ab-positive, and this risk increased with age. Based on our data, we conclude that abnormal TSH and TPO-Ab are common in pregnant women of the Samara region. Given the association of thyroid dysfunction to adverse pregnancy outcomes, screening of this population for abnormal thyroid function should be considered.     

甲状腺自身抗体阳性对IVF-ET妊娠结局的影响

目的探讨甲状腺自身抗体阳性对体外受精-胚胎移植(IVF-ET)妊娠结局的影响。方法选取我院收诊的72例行IVF-ET治疗的不孕妇女作为研究对象,按照血清甲状腺自身抗体检测结果分为观察组(36例,阳性)和对照组(36例,阴性)。比较两组的性激素水平和妊娠结局;分析甲状腺自身抗体阳性和妊娠率、优胚率、卵裂率、获卵数及IVF受精率的相关性。结果观察组的早期流产率高于对照组(P<0.05)。观察组和对照组的E2、LH、FSH水平及妊娠率、优胚率、卵裂率、获卵数、IVF受精率比较,差异均无统计学意义(P>0.05)。患者的TPO-Ab、TG-Ab和IVF受精率均呈负相关(P<0.05)。结论甲状腺自身抗体呈阳性会对行IVF-ET不孕妇女的妊娠结局产生不良影响,临床应进行有效预防及治疗。     

甲状腺过氧化物酶抗体在冠心病患者中的检测价值

目的探讨甲状腺过氧化物酶抗体(TPO-Ab)在冠心病患者中的检测价值。方法回顾性分析2014年1月至2015年12月该院行冠状动脉造影检查的240例患者,左冠状动脉投射4个体位,右冠状动脉投射2个体位,记录造影结果,并采用Gensini积分法进行评定;相关性分析采用Spearman法。结果 TPO-Ab滴度增高共计16例,其中冠状动脉粥样硬化组2例,冠状动脉粥样硬化性心脏病组14例;TPO-Ab阳性患者Gensini积分为(39.56±10.23)分,与TPO-Ab阴性[(17.20±8.85)分]相比,差异具有统计学意义(P0.05);TPO-Ab与Gensini积分呈正相关(r=0.1132,95%CI为0.099 8~0.269 1,P0.05)。结论甲状腺功能正常的人群,TPO-Ab增高可能会提高冠心病的发病风险,并且伴有更严重的冠脉狭窄。     

常规超声联合血甲功检查诊断甲状腺结节的效果分析

目的:探讨常规超声联合血甲功检查诊断甲状腺结节的效果。方法:选取我院2019年1月—2020年6月收治甲状腺结节患者110例;所有患者都接受常规超声检查联合血甲功,根据检查结果的不同对患者进行分组,共分为79例对照组(良性)和31例观察组(恶性),对比检查结果。结果:观察组边界模糊、淋巴肿大、不规则形态、钙化以及不均匀回声等情况(14例、3例、17例、21例、12例)多于对照组(13例、1例、15例、20例、10例),观察组T S H(2.8±0.1)mIU/L水平显著高于对照组(2.1±0.2)mIU/L,差异有统计学意义(P<0.05)。观察组TT3、TT4、TPO-Ab、TGA和对照组相比无明显差异(P>0.05)。结论:常规超声联合血甲功检查有助于甲状腺结节临床检查诊断结果精确性提高,应用效果明显。     

1884例住院糖尿病患者甲状腺过氧化物酶抗体阳性率检测分析

目的 探讨住院糖尿病患者甲状腺过氧化物酶抗体(TPO-Ab)阳性率情况.方法 对1508例2型糖尿病(T2DM)、76例典型1型糖尿病(T1DM)、44例成人隐匿性自身免疫糖尿病(LADA)和256例未分型糖尿病(NTDM)患者进行TPO-Ab检测,并做好临床资料的登记和分析比对.结果 ①T2DM患者TPO-Ab的阳性率为14.59%(220/1508),典型T1DM组为42.11%(32/76)、NTDM组为31.25%(80/256)、LADA组为63.64%(28/44),与LADA组相比差异有统计学意义(P均＜0.05),而与典型T1DM、NTDM组间差异无统计学意义(P均＞0.05).②LADA与NTDM组相比,年龄、病程、BMI差异均无统计学意义(P均＞0.05).结论 ①NTDM组TPO-Ab阳性率高于T2DM组,但低于典型T1DM组和LADA组.②LADA组的患者TPO-Ab阳性率最高,提示LADA与自身免疫甲状腺炎患病机制的共同性.③TPO-Ab的阳性率从T2DM、NTDM、典型T1DM及LADA呈连续递增趋势.

Abstract：

Objective To investigate the positive rate of thyroid peroxidase antibody(TPO-Ab) in the diabetic in-patients. Methods The positive rates of thyroid peroxidase antibody ( TPO-Ab ) was measured in 1884 subjects, including 1508 type 2 diabetes mellitus ( T2DM ), 76 classic typetype 1 diabeties( T1 DM ), 44 latent autoimmune diabetes in adults (LADA) and 256 "untype" diabetics ( NTDM ). Clinical data were registered and analyzed. Results( 1 )The positive rates of TPO-Ab were 14. 59％ in T2DM(220/1508) ,42. 11％ in T1DM (32/76) ,31.25％ in NTDM ( 80/256 ), 63.64％ in LADA group ( 28/44 ), respectively. The positive rate of T2DM was significantly lower than that of LADA ( P ＜ 0. 05 ), but no significant difference was found in the coparison between T1 DM and NTDM ( P ＞ 0. 05 ). ( 2 ) There were no significant differences between LADA and NTDM groups in the comparison of age, course of disease and BMI ( Ps ＞ 0. 05 ). Conclusion ①The positive rate of TPO-Ab in NTDM was significantly higher than that of T2DM, whereas significantly lower than that of T1 DM and LADA.②The positive rate of TPO-Ab was highest in LADA, which indicate that LADA and AIT might have the common etiological mechanisms. ③There is a consisted increasing trend in the positive rate of TPO-Ab from T2DM ,NTDM,and classic T1DM to LADA.     

脑出血后抑郁症状与甲状腺功能相关研究

目的研究脑出血患者急性期抑郁症状与甲状腺功能的关系。方法选择首次发病的急性脑出血患者100例,根据抑郁自评量表（SDS）和汉密尔顿抑郁量表（HAMD）评分结果分为伴抑郁组和无抑郁组,分析2组患者血清三碘甲状腺原氨酸（T3）、甲状腺素（T4）、游离三碘甲状腺原氨酸（FT3）、游离甲状腺素（FT4）、促甲状腺激素（TSH）、抗甲状腺过氧化物酶抗体（TPO-Ab）和抗甲状腺球蛋白抗体（TG-Ab）水平。结果伴抑郁组患者血清FT3和TSH水平显著低于无抑郁组,FT4显著高于无抑郁组（P〈0.01）;血清T3、T4、TPO-Ab、TG-Ab水平伴抑郁组与无抑郁组之间差异无统计学意义（P〉0.05）。结论急性脑出血患者抑郁症状可能与甲状腺功能变化有关,血清FT3、FT4和TSH可作为评价急性脑出血患者抑郁症状的客观指标。     

Sensitive enzyme-linked immunosorbent assay for measurement of autoantibodies to human thyroid peroxidase.

The development of a sensitive assay for detection of autoantibodies against one of the major thyroid antigens, thyroid peroxidase (TPO), is described. TPO was purified from human thyroid tissue by: (1) isolation of thyroid microsomes using homogenization and differential centrifugation, (2) solubilization of membrane proteins by Zwittergent 3-14, and (3) anion exchange liquid chromatography on a FPLC Mono Q column. Autoantibodies against TPO (TPO-Ab) were measured using an enzyme-linked immunosorbent assay (ELISA) with serum samples diluted 1:100. Standards containing 70, 7, 0.7, 0.02 and 0 U ml-1 TPO-Ab were employed (reference standard code 66/387 NIBSC, London, UK). The detection limit was 0.02 U ml-1 corresponding to 2 U ml-1 in undiluted serum. The inter- and intra-assay coefficients of variation were 8.6% and 5.3%. In 109 healthy control subjects TPO-Ab was found in 9 (8.3%), while 43 (97.7%) out of 44 patients with newly diagnosed untreated Graves' disease had detectable TPO-Ab in serum. All of 16 patients with newly diagnosed spontaneously developing primary hypothyroidism had circulating TPO-Ab (range 16-7000 U ml-1). The new assay is a valuable tool for evaluation of thyroid autoimmunity in individual patients and for studying the epidemiology of thyroid autoimmunity.     

女性系统性自身免疫疾病合并甲状腺功能异常分析

目的分析原发性干燥综合征(pSS)与系统性红斑狼疮(SLE)女性患者甲状腺功能异常发生情况,以及甲状腺抗体(ATA)的检出率。方法回顾性分析100例pSS、98例SLE及153例健康对照组(HC)的甲状腺功能、ATA。结果 pSS组与SLE组甲状腺功能异常率均较HC组明显升高;pSS组最常见的是亚临床甲减,而SLE组为低T3综合征。pSS组患者抗甲状腺抗体(TPOAb与TgAb)检出率均较HC组明显升高,SLE患者仅TPOAb阳性率较HC组有统计学差异。pSS组TgAb检出率较SLE组更高。结论 pSS与SLE患者更易出现甲状腺功能的异常,ATA的检出率更高。     

Establishing thresholds and effects of gender,age, and season for thyroglobulin and thyroid peroxidase antibodies by mining real-world big data

BackgroundThyroglobulin antibody (TG-Ab) and thyroid peroxidase antibody (TPO-Ab) are cornerstone biomarkers for autoimmune thyroid diseases, and establishment of appropriate thresholds is crucial for physicians to appropriately interpret test results. Therefore, we established the thresholds of TG-Ab and TPO-Ab in the Chinese population through analysis of real-world big data, and explored the influence of age, gender, and seasonal factors on their levels.MethodsThe data of 35,869 subjects downloaded from electronic health records were analyzed after filtering based on exclusion criteria and outliers. The influence of each factor on antibody levels was analyzed by stratification. Thresholds of TG-Ab and TPO-Ab were established through Clinical Laboratory Standards Institute document C28-A3 and National Academy of Clinical Biochemistry (NACB) guidelines, respectively.ResultsThere were significant differences according to gender after age stratification; the level of TG-Ab gradually increased with age in females. There were significant differences in TG-Ab and TPO-Ab distributions with respect to age after gender stratification. Moreover, differences were observed between seasons for TG-Ab and TPO-Ab. The thresholds of TG-Ab and TPO-Ab were 107 [90% confidence interval (CI):101–115] IU/mL and 29 (90% CI: 28–30) IU/mL, respectively, using C28-A3 guidelines, but were 84 (90% l CI: 50–126) IU/mL and 29 (90% CI: 27–34) IU/mL, respectively, using NACB guidelines.ConclusionThe levels of TG-Ab and TPO-Ab were significantly affected by gender, age, and season. The thresholds for TG-Ab and TPO-Ab for the Chinese population were established by big data analysis.