Expression of integrin-α<Subscript>3</Subscript> mRNA in meningiomas and its correlation with proliferation and invasion

To investigate the expression of integrin-α3 mRNA in meningiomas and its correlation with proliferation and invasion, the expression of integrin-α3 subunit was detected by using in situ hybridization in patients with meningiomas （36 cases） and normal dura （2 cases） and arachnoid tis- sues （2 cases）. SABC immunohistochemical assay was used to study the expression of Ki-67 nuclear antigen. The results showed that the expression of integrin-% mRNA in benign, atypical and malig- nant meningiomas was 2.52±0.362, 1.75±0.316 and 1.42±0.633, respectively. The expression levels of integrin-α3 mRNA was significantly lower in atypical or malignant meningiomas than those in benign meningiomas （P〈0.05, P〈0,01, respectively）. The expression of integrin-α3 mRNA in those with invasive biological behavior was lower than that in those without invasion （1.63±0.462 vs. 2.61±0.526, P〈0.01）. Moreover, the expression of integrin-α3 mRNA was inversely associated with Ki-67 labeling index in all cases. It suggested that integfin-α3 subunit participated in the modulatory process of growth of meningiomas. The proliferation activity and malignant grade of meningiomas were increased with the decreased expression of integrin-α3 subunit, and the down-regulation of integrin-α3 mRNA was associated with the invasive biological behaviors in meningiomas.     

Effects of down-regulation of integrin-β<Subscript>1</Subscript> expression on migration and hepatic metastasis of human colon carcinoma

Organ-specific tumor cell adhesion to extracellular matrix (ECM) components and cell migration into host organs often involve integrin-mediated cellular processes. Direct integrin-mediated cell adhesion to ECM components in the space of Disse appears to be required for the successful liver metastatic formation of colon cancer. In the present study, human colon cancer HT-29 cells were transfected by liposome with integrin-β1 antisense oligodeoxynucleotide (ASODN). The integrin-β1 gene expression in HT-29 cel...     

Public Anatomies in <Emphasis Type="Italic">Fin</Emphasis>-<Emphasis Type="Italic">de</Emphasis>-<Emphasis Type="Italic">Siècle</Emphasis> Vienna

Anatomical exhibitions, online atlases and televised dissections have recently attracted much attention and raised questions concerning the status of and the authority over the human body, the purpose of anatomical education within and outside medical schools and the methods of teaching in the digital age. I propose that for understanding the current public views of anatomy, we need to gain insight into their historical development. This article focuses on anatomies accessible to non-medical audiences in the capital of the Habsburg Empire, Vienna, at the time when the city was the seat of a world-leading medical school. Anatomy at the University of Vienna was famous for its research, instruction and the abundant provision with dissectible corpses. Public anatomies were equally rich and ranged from exhibitions at the Präuscher’s Panoptikon und Anatomisches Museum, established in 1871 in the Prater amusement park, lectures on human and comparative anatomy by the university professor Carl Bernhard Brühl (1863–1890), to displays of anatomical objects at the World Exhibition in 1873. I finish by discussing a collection of letters written by the prospective ‘cadaver donors’, offering an insight into the ways in which medical encounters and anatomical knowledge informed the working-class views of their bodies. By looking at the kinds of anatomy in circulation, as well as at the participants in these exchanges, I want to illuminate the relationship between academic and public anatomies, as well as to reveal the purposes to which public anatomy served.     

精浆β-内啡肽和前列腺素E₂与慢性盆腔疼痛综合征疼痛症状关系的研究

目的 探讨精浆中β-EP和PGE₂与CPPS疼痛症状的相关性。方法 本研究选择符合诊断标准的A组(NIH-CPSI疼痛评分≥14分)、B组(NIH- CPSI疼痛评分＜14分)各30例CPPS患者,同时设正常组24例。采用ELISA法检测精浆中β-EP和PGE₂浓度,并对疼痛症状与精浆中β-EP和PGE₂相关性进行分析。结果 A组,B组β-EP浓度均低于正常组( P均＜0.05);且A组低于B组( P＜0.05)。 A组,B组PGE₂浓度均高于正常组( P均＜0.05);且 A组高于B组( P＜0.05)。结论 CPPS疼痛症状与患者精浆中β-EP和PGE₂浓度有关,疼痛症状越明显,β-EP浓度越低,PGE₂浓度越高。     

<Emphasis Type="Italic">Erysipelothrix rhusiopathiae</Emphasis> endocarditis: A preventable zoonosis?

Background Erysipelothrix rhusiopathiae is a bacterium ubiquitous in the environment. It can cause a variety of diseases and the risk of infection is closely related to the level of occupational exposure to infected or colonised animals. Aims To discuss the clinical features and treatment of this zoonosis, to increase awareness of this pathogen and to emphasise the need for meticulous attention to hygienic work practices in reducing the risk of infection. Method A case report of a farmer withE. rhusiopathiae endocarditis and the management of the infection. Results The patient was successfully treated with valve replacement surgery and antimicrobial therapy. Conclusions Early identification of this microorganism is essential for appropriate treatment of endocarditis. Greater awareness and safe work practices can help reduce the risk of human infection by this microorganism.     

What is new in the management of <Emphasis Type="Italic">Helicobacter pylori</Emphasis>?

Since the discovery of Helicobacter pylori in the early 1980s several treatment regimens have been developed to effectively treat this infection. International guidelines have allowed consensus on the best management and improved eradication rates. In recent years increasing antimicrobial resistance and falling eradication rates highlight the need for updated guidelines. In this article we review the most recent European management guidelines and in view of the unavailability of some drugs consider new treatment regimens for the management of H. pylori in Ireland.     

A Diagnostic Methodology for <Emphasis Type="Italic">Hazy</Emphasis> Data with “Borderline” Cases

Several illnesses like the hypertension, the dementia among others in reality contain borderline cases. These “borderline” (alternately mentioned hazy) cases are neither labeled healthy nor diseased by the medical professionals. Consequently, the current diagnostic test methodology is inappropriate for data with the borderline cases. A new methodology is greatly needed to analyze and interpret diagnostic test data with the borderline cases. In this article, a new methodology is therefore developed, discussed, and illustrated. The medical parameters: the sensitivity, the specificity, and the disease prevalence of the sampled participants are calculated and interpreted using the new methodology with data on borderline dementia and blood pressure cases separately.     

Expression and location of intracellular tissue factor in atherosclerosis stable plaque of ApoE<Superscript>−/−</Superscript> mice

In the ApoE^-/- mouse model of atherosclerosis （AS） stable plaque, the expression and location of intracellular tissue factor （TF） in the cellular components of AS stable plaque were investigated in order to explore the cellular mechanism of AS thrombosis. Pathological changes of the stable plaque were observed under a microscope. The expression of TF protein was examined in aortic stable plaque of mice by using immunohistochemistry. Color image planimetric system was used to analyze the histological components of the stable plaque and the TF distribution. Under the confocal microscope, the intracellular TF location in the stable plaque of mice was observed. The results showed the cellular area was the major part of stable plaque （67.36%±6.52%, P〈0.01）. The percentage of total area occupied by cellular area was significantly larger than atheromatous gruel and acellular area （P〈0.01）. Macrophages and smooth muscle cells （SMC） were major cells in the cellular area. The percentage of total area occupied by SMC was significantly larger than by macrophages （P〈0.01）. Multiple linear regression analysis showed there was a positive correlation between TF area and SMC area （r=0.616, P=-0.008）, and no correlation was found between TF area and macrophage area （r=0.437, P=0.08）. Pictures of color image planimetric analysis of TF and SMC were merged to highlight areas with co-localization （yellow）, it was concluded that the process could be a cell-mediated TF expression in the stable plaque. SMC may be the major source of TF in AS without plaque rupture.     

谈中医院校<病理学>教学

中医院校的病理教学中,应该注意帮助学生明确病理学在中西医结合发展中的地位和作用,并就如何提高教学质量,如何调动学生的主观能动性等问题,介绍了自己的经验和见解.     

<中国医药导报>来稿要求

《中国医药导报》杂志是卫生部主管、中国医学科学院主办的集医药政策、市场、经营、管理、科研、教育、临床报道于一体的国家医药卫生期刊,是国家中国核心期刊(遴选)数据库、中国期刊全文数据库、     

浅议<伤寒论>中温阳法的运用

仲景用温阳法或单独应用或与其他方法合而应用,法随证变,意境无穷.具体来说有宣通胸阳.温补中阳.通达陷阳、汪通经脉及回阳教逆等不同.     

对专科生<方剂学>教学的思考

目的不断改进<方剂学>教学方法,提高教学质量,达到预期教学效果.方法巩固专业思想,突出配伍特点及组方方法,明确用方指征,注重活跃课堂气氛.结果掌握方剂组方原则及应用规律.结论注重教学方法和教学手段,有助于专科生更好地掌握方剂的基本理论和组方能力,为临床实践打下坚实的基础.     

谈<金匮要略>致瘀十三因

<金匮>对瘀血证治论述极为丰富,创立了许多著名活血化瘀方药,如大黄虫丸、抵当汤、下瘀血汤、温经汤等.近年学者对<金匮>瘀血证治等内容进行了广泛的研究,但对致瘀之因谈及很少.本文结合<金匮>原文精神对致瘀原因从十三个方面进行了探讨,试图进一步阐明瘀血的发病原因与机理,以及相应的立法组方用药.     

超敏C反应蛋白<2mg/L的心绞痛患者抗血小板聚集治疗方案探讨

目的 探讨两种方法 治疗超敏C反应蛋白(hs-CRP)<2 mg/L的心绞痛患者的疗效与预后.方法选择因心绞痛6 h~48 h来我院治疗hs-CRP<2 mg/L的患者共122例,分为对照组和观察组各61例.对照组采用拜阿司匹灵,观察组加用硫酸氢氯吡格雷,对比2组患者的疗效及预后情况.结果 2组患者的治疗有效率和预后对比无差异(P>0.05).结论 针对hs-CRP<2 mg/L的心绞痛患者采用阿司匹林单独治疗较联合硫酸氢氯吡格雷治疗的疗效和预后对比无差异.