丙型肝炎患者肝组织免疫组织化学研究

目的　研究丙型肝炎 (丙肝 )病毒 (HCV)抗原在丙肝患者肝组织中的分布状况及其相互关系。方法　采取免疫组织化学方法 ,用多克隆抗 -HCV和单克隆抗 -HCV -NS3、抗 -HCV -NS5 ,对 10 7例丙肝患者肝组织中HCV抗原进行检测。结果　应用 3种抗体均从丙肝患者肝组织中检出相应抗原 ,检出率分别为 38.3%、2 8.0 %和 5 2 .3% ;阳性颗粒定位于肝细胞浆 ;阳性细胞分布呈散在、弥漫状和簇状 ;HCV -NS3的表达与炎症反应无明显解剖学关系 ,HCV -NS5阳性细胞较常见位于炎症灶旁或灶中。结论　HCV各成分在肝组织中的表达水平存在差别 ;HCV -NS5的表达可能与丙型肝炎的发病机理有关

Immunohistochemical findings on liver tissue in patients with hepatitis C

Objective　To investigate thedistributions and infer relations of HCV antigens in the liver tissues. Methods　Immunohistochemicaltechniques as polyclonal anti body to HCV, monoclonal antibody to HCV-NS3 and monoclonalantibody to HCV-NS5 were used to detect HCV antigens in 107 patients with hepatitis C. Results　HCVantigens in liver tissues were identified on these three kinds of an tibodies. Thepositive rates were 38.3%,28.0% and 52.3% respectively. The positi ve granules werelocated in the cytoplasma of liver cells. The distributions of positive cells werescattered, diffusive and clustered. No obvious anatomic association was observed betweenthe expression of HCV-NS3 and inflammatory reac tion. HCV-NS5 positive cells were morecommonly seen around or in the flammatory focci . Conclusion　Theexpressions of various components of HCV were demonstrated in different concentrations.The expressions of HCV-NS5 might be related to the pathogenesis of hepatitis C.