山东省部分地区献血员丙型肝炎病毒感染状况及分型研究

选择424例有偿献血员用ELISA检测抗-HCV,对抗-HCV阳性血清用ELISA进行血清学分型,对己确定血清型者采用RT-PCR作基因分型.结果424例献血员中,HCV感染率为21.70%(92/424),献全血并血浆者的HCV感染率(45.14%)显着高于只献全血者(6.94%);92例抗-HCV阳性者中,血清学分型率为34.78%(32份),其中血清学Ⅰ型30例(93.75%)、Ⅱ型2例(6.25%):该32例血清中有30例可用基因分型,其中基因Ⅱ型26例(86.67%)、Ⅲ型3例(10%)、Ⅱ/Ⅲ型混合感染1例(3.33%),两种分型方法的一致率为93.33%(28/30).表明山东省部分地区献血人群的HCV感染率较高;献血浆是该献血人群HCV感染的危险因素;其HCV感染以血清学I型和基因Ⅱ型为主.

A Study on Prevalence and Subtypes of Hepatitis C Virus among Blood Donors in Some Areas of Shandong Province

To investigate the distribution of HCV infection and subtypes among blood donors in some areas of Shandong Province. MethodSerum samples were collected from 424 paid blood donors. Anti-HCV was detected by enzyme-linked immunosorbent assay (ELISA).Serotypes of anti-HCV positive sera were identified by ELISA and genotypes were determined by RT-PCR.ResultsThe HCV prevalence of 424 blood donors was 21.70% (92/424). It was significantly higher in the donors donating blood and plasma (45.14%) than in the donors with only blood donation (6.94%). Of 92 anti-HCV positive sera detected, 32 (34.78%) were typed by ELISA. 30 (93.75%) were serotype Ⅰ, and 2 (6.25%) serotype Ⅱ. Of these 32 sera, 30 were determined by RT-PCR genotype method. HCV genotypes Ⅱ,Ⅲ and Ⅱ/Ⅲ were 26 (87.67%),3(10%), and Ⅰ(3.33%), respectively. The coincidence rate of the two methods was 93.33% (28/30).ConclusionThe prevalence of HCV among blood donors in some areas of Shandong Province was relatively high. Plasma-donation was a risk factor of HCV infection in blood donors.The prevalent subtypes of HCV were serotype Ⅰ and genotype Ⅱ.