高效液相色谱法测定叶下珠中没食子酸的含量

以高效液相色谱法测定叶下珠中没食子酸的含量。采用YWG C18( 10 μm)色谱柱 ,甲醇—水—磷酸 ( 4∶ 96∶ 0 0 5)为流动相 ,测得叶下珠中没食子酸的含量为 0 114% ,平均回收率为 10 3.1%。测定方法快速、灵敏 ,具有实用价值     

四物汤对红细胞免疫及骨髓干细胞增殖能力的影响

用四物汤对辐射损伤小鼠进行治疗.结果表明:电离辐射对小鼠红细胞免疫功能及骨髓干细胞增殖能力有明显的抑制作用,应用四物汤治疗后红细胞免疫功能得到恢复,说明四物汤有较明显的增强红细胞免疫功能的作用.由骨髓干细胞增殖实验中可看出四物汤可使受照小鼠的骨髓干细胞增殖能力恢复正常.该研究为四物汤治疗辐射损伤引起的红细胞免疫功能及造血功能低下提供了实验依据.     

基于不完整数据的IHB-LightGBM心脏病预测模型

提出基于不完整数据的IHB-LightGBM(Improved Hyperband-Light Gradient Boosting Machine)心脏病预测模型。首先,在Hyperband算法超参数采样的基础上引入了权重值,并通过蓄水池法按特征权重对其进行排序,从而筛选出最优参数以提高算法的参数寻优能力;其次,针对心脏病数据样本小且属性缺失的问题,使用K近邻算法对不完整数据进行缺失值插补,再将处理得到的完整数据进行归一化,使数据映射至0～1范围内;最后,对LightGBM采用改进后的IHB优化算法进行全局参数寻优,建立IHB-LightGBM心脏病预测模型。使用UCI心脏病数据集进行实验,结果表明IHB算法的参数寻优效果优于贝叶斯、随机搜索等优化算法,IHB-LightGBM模型在各项评价指标也上明显高于随机森林、极端随机树等算法,可以获得更快的预测速度和更高的预测精度。     

CR假体与PS假体全膝关节置换治疗膝骨性关节炎合并膝外翻畸形的疗效分析

目的 比较后交叉韧带保留型（CR）假体与后方稳定型（PS）假体行人工全膝关节置换术（TKA）治疗膝骨性关节炎合并膝外翻畸形的临床疗效。方法 回顾性分析南昌大学附属赣州医院关节外科2019年5月至2021年5月收治的60例（60膝）膝骨性关节炎合并膝外翻畸形患者资料,均为单侧置换。30例采用CR假体行TKA治疗（CR组）,30例采用PS假体行TKA治疗（PS组）。比较两组患者的手术时间、术中失血量、术后引流量、术后3 d血红蛋白（Hb）下降量及深静脉血栓发生情况;比较两组患者手术前后膝外翻角;比较两组患者术后1周、1个月、3个月、6个月、1年疼痛视觉模拟评分（VAS）、膝关节活动度（ROM）、美国特种外科医院膝关节评分（HSS）。结果 60例患者均顺利完成TKA手术,所有患者随访13 ～ 28个月,平均（18.51±0.90）个月。CR组术中出血量、术后引流量、术后3 d的Hb下降量较PS组减少（P＜0.05）;两组手术时间相当、术后均无深静脉血栓发生,差异无统计学意义（P＞0.05）;两组手术前后膝外翻角的比较差异无统计学意义（P＞0.05）;CR组术后1周、1个月VAS评分较PS组更低（P＜0.05）,两组术后3个月、6月、1年VAS评分比较差异无统计学意义（P＞0.05）;CR组术后1周、1个月、3个月膝关节ROM和HSS评分优于PS组（P＜0.05）,两组术后6个月、1年膝关节ROM和HSS评分比较差异无统计学意义（P＞0.05）。结论 采用CR或PS假体行TKA手术治疗膝骨性关节炎合并膝外翻畸形均可有效纠正膝关节畸形、减轻膝关节疼痛、改善膝关节活动度及功能,取得满意临床疗效;但相对PS假体,CR假体保留了后交叉韧带,减少了股骨髁部截骨量,从而减少手术出血,更好减轻早期术后疼痛,有助于TKA术后早期功能康复。     

FUNCTIONAL MAPPING OF THE HUMAN BRAIN DURING ACUPUNCTURE WITH MAGNETIC RESONANCE IMAGING SOMATOSENSORY CORTEX ACTIVATION

1NTRODUCTIONHegu(Ll4)isoneofthemosteffectiveandmostfrequentlyusedacupointsintraditionalChineseacupuncture.ItisindicatedfOrpaininmanypartsofthebody,butisparticularlyeffec-tiveforheadache,migraine,toothache,sorethroatandotherd1sordersoftheheadandface.Manyanimalexperimentsindicatethathighercentersinthenervoussystemareinvolvedinacupunctureanalgesia["2]butdirectevidenceforhumansubjectsisdifficulttoobtainuntilthere-centadventofpowerfu1andn0n-invasivemeth-odsforneuroimaginginthe9O's.Werecentlya…     

Ginsenoside Rs3, a genuine dammarane-glycoside from Korean red ginseng

A genuine dammarane-glycoside, named as ginsenoside Rs₃, was isolated from the MeOH extracts of Korean red ginseng (Panax ginseng C.A. Meyer) through repeated silica gel column chromatographies and its chemical structure was determined as (20S)-protopanaxadiol 3-O-[6″-O-acetyl-β-D-glucopyranosyl (1→2)-β-D-glucopyranoside on the basis of several spectral and physical evidences including HMBC and FAB-MS.     

Polymeric Microspheres Prepared by Spraying into Compressed Carbon Dioxide

Purpose. The objective was to prepare polymeric microparticles by atomizing organic polymer solutions into a spray chamber containing compressed CO₂ (PCA-process) and to study the influence of various process parameters on their morphological characteristics. Methods. The swelling of various pharmaceutically acceptable polymers [ethyl cellulose, poly(methyl methacrylate), poly(-caprolactone), poly(dl-lactide), poly(l-lactide) and poly(dl-lactide-glycolide) copolymers] in CO₂ was investigated in order to find polymers which did not agglomerate during the spraying process. Poly(l-lactide) (L-PLA) microparticles were prepared by spraying the organic polymer solution into CO₂ in a specially designed spraying apparatus. The effect of various process (pressure and temperature of the CO₂ phase, flow rate) and formulation (polymer concentration) variables on the morphology and particle size of L-PLA-microparticles was investigated. Results. Polymers with low glass transition temperatures agglomerated even at low temperatures. The formation of microparticles was favored at moderate temperatures, low polymer concentrations, high pressures and high flow rates of CO₂. High polymer concentrations and low flow rates resulted in the formation of polymeric fibers. Colloidal L-PLA particles could also be prepared with this technique in a surfactant-free environment. Initial studies on the microencapsulation of drugs resulted in low encapsulation efficiencies. Conclusions. The PCA method is a promising technique for the preparation of drug-containing microparticles. Potential advantages of this method include the flexibility of preparing microparticles of different size and morphology, the elimination of surfactants, the minimization of residual organic solvents, low to moderate processing temperatures and the potential for scale-up.     

Microsomal casein kinase II in endoplasmic reticulum- and Golgi apparatus-rich fractions of ROS 17/2.8 osteoblast-like cells: An enzyme that modifies osteopontin

Osteopontin is an acidic phosphoprotein containing casein kinase II (CKII) phosphorylatable sites and an acidic amino acid cluster. The metabolically ³²P-labelings of both serines and threonines in vitro in osteopontin immunoprecipitated from rat osteoblast-like ROS 17/2.8 cells may suggest that casein kinase II catalyzes this modification. The enzyme occurs in microsomal fractions of rat osteoblast-like ROS 17/2.8 cells. Subcellular fractions containing endoplasmic reticulum and Golgi apparatus were isolated by differential centrifugation and were identified according to their ultrastructures and the presence of marker enzymes such as glucose-6-phosphatase and thiamine pyrophosphatase, respectively. Both fractions phosphorylated the partially dephosphorylated osteopontin and the specific substrate peptide RRREEETEEE. Endoplasmic reticulum-catalyzed peptide phosphorylation was 2.7 times lower than that of Golgi although both endoplasmic reticulum- and Golgi-catalyzed peptide reactions were 50% inhibited by 20 and 100 ng/ml heparin, respectively. Western blot analysis revealed that both fractions contained osteopontin and microsomal CKII. Furthermore, microsomal CKII was immunogold-labeled in endoplasmic reticulum and Golgi apparatus. Heparin inhibition and utilization of [-³²P]GTP as a phosphate donor by both fractions confirmed their capacity to phosphorylate osteopontin. The results suggest that microsomal CKII modifies the acidie matrix proteins during transportation. These matrix phosphoproteins may participate in the mineralization process of hard tissues.     

天津市1999-2021年0~14岁儿童伤害死亡谱及城乡差异分析

目的 分析天津市0~14岁儿童伤害死亡谱的特征、变化情况及城乡差异。方法 1999-2021年天津市儿童伤害死亡数据来源于天津市全人口全死因监测数据库,计算不同亚组人群和主要伤害原因的构成比、粗死亡率和标化死亡率并比较城乡差异。采用Cochran-Armitage趋势检验分析死亡原因构成比的时间变化趋势。采用Joinpoint回归分析变化趋势,计算平均年变化百分比（AAPC）。伤害死亡风险的季节差异用死亡率比值及其95%CI表示。结果 1999-2021年,伤害是天津市0~14岁儿童的第3位死因。农村儿童死于医疗卫生机构的比例为31.08%,低于城市的37.82%。儿童伤害的总体标化死亡率呈下降趋势（AAPC=-5.54%,P<0.001）。溺水和道路交通伤害的标化死亡率在城市和农村地区呈下降趋势（P<0.001）。意外中毒的标化死亡率仅在农村地区呈下降趋势（AAPC=-8.09%,P<0.001）,在城市地区无明显变化趋势（P>0.05）。自杀标化死亡率在城市地区无明显变化趋势（P>0.05）,在10~14岁农村儿童中呈上升趋势（AAPC=4.58%）。跌倒/坠落标化死亡率在城市和农村地区均无明显变化趋势（P>0.05）。伤害死亡的总体风险和溺水死亡风险在城乡均为夏季最高;道路交通伤害在城市为秋季最高,在农村为夏季最高;意外中毒死亡风险在城乡均为冬季最高。结论 近年来天津市儿童伤害死亡情况得到明显改善。城市和农村地区的儿童伤害死亡水平仍存在较大差异,在未来政策制定中,应充分考虑缩小城乡差距。     

矽肺相关蛋白的纯化,鉴定及其促成纤维细胞增殖作用

目的分离、鉴定与矽肺发生、发展过程相关的几种蛋白质,并测定其促成纤维细胞增殖的活性。方法采用高压液相色谱技术包括凝胶过滤柱层析、离子交换柱层析、反相Ｃ４高压液相色谱柱层析,分别对矽肺大鼠肺泡灌洗液（ＢＡＬＦ）、肺泡巨噬细胞冻融物（ＡＭＥ）及其条件培养液（ＡＭＣＭ）进行分离纯化,并通过Ｎ末端氨基酸序列的测定来鉴定这些蛋白质。结果（１）染尘２１天后,矽肺大鼠ＢＡＬＦ中白蛋白含量明显升高;（２）从ＢＡＬＦ、ＡＭＥ和ＡＭＣＭ中除纯化到相对分子质量为１５４００的溶菌酶外（其刺激成纤维细胞增殖的活性为１４０％）,还纯化到一种新的具有明显促成纤维细胞增殖的细胞因子（Ｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｐｒｏｍｏｔｉｎｇｆａｃｔｏｒ,ＦＧＰＦ）,其最适作用浓度为２．０～６．０μｇ／ｍｌ。结论巨噬细胞分泌的ＦＧＰＦ和溶菌酶在矽肺纤维化过程中可能有重要作用。