外周血中BAFF、BAFF-R及Bcl-xL的表达与B细胞非霍奇金淋巴瘤疾病进展的相关性研究 |
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引用本文: | 刘静,蔡欣,付文亮,夏文戎,苏航,张伟京,徐东刚.外周血中BAFF、BAFF-R及Bcl-xL的表达与B细胞非霍奇金淋巴瘤疾病进展的相关性研究[J].军事医学,2012,36(3):209-212. |
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作者姓名: | 刘静 蔡欣 付文亮 夏文戎 苏航 张伟京 徐东刚 |
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作者单位: | 刘静 (军事医学科学院附属医院淋巴瘤科,北京,100071) ; 蔡欣 (军事医学科学院基础医学研究所,北京,100850) ; 付文亮 (军事医学科学院基础医学研究所,北京,100850) ; 夏文戎 (军事医学科学院基础医学研究所,北京,100850) ; 苏航 (军事医学科学院附属医院淋巴瘤科,北京,100071) ; 张伟京 (军事医学科学院附属医院淋巴瘤科,北京,100071) ; 徐东刚 (军事医学科学院基础医学研究所,北京,100850) ; |
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基金项目: | 国家自然科学基金资助项目 |
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摘 要: | 目的研究B细胞性非霍奇金淋巴瘤(B-NHL)患者外周血B cell-activating factor(BAFF)、其受体BAFF-R及Bcl-xL基因的表达,探讨其与B-NHL疾病进展的相关性。方法应用实时荧光定量PCR法检测36例B-NHL患者外周血单个核细胞(PBMC)BAFF、BAFF-R、Bcl-xL mRNAs的相对表达水平,以7例健康献血员作为对照,并据性别、淋巴瘤国际预后指数(IPI)、年龄、临床分期、是否耐药进行分组比较。结果 B-NHL患者PBMC BAFF、BAFF-R及Bcl-xL的mRNAs表达较正常对照组显著升高(P<0.05),Ⅲ/Ⅳ期高于Ⅰ/Ⅱ期,耐药B-NHL组高于非耐药组(P<0.05)。结论外周血单个核细胞BAFF、BAFF-R、Bcl-xL mRNAs的表达水平与B-NHL临床分期有关,在耐药B-NHL外周血中明显升高,提示BAFF/BAFF-R介导的信号通路可能参与B-NHL的临床进展及耐药机制。
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关 键 词: | B细胞性非霍奇金淋巴瘤 耐药性 肿瘤 基因表达 BAFF BAFF-R Bcl-xL B细胞激活因子 受体 B细胞活化因子 |
Correlation between expressions of BAFF,BAFF-R and Bcl-xL in peripheral blood and progression in B cell non-Hodgkin′s lymphoma patients |
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Authors: | LIU Jing CAI Xin FU Wen-liang XIA Wen-rong SU Hang ZHANG Wei-jing XU Dong-gang |
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Institution: | 1.Department of Lymphoma,Affiliated Hospital,Academy of Military Medical Sciences,Beijing 100071,China;2.Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850,China) |
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Abstract: | Objective To detect the expressions of BAFF,BAFF-R and Bcl-xL in peripheral blood from patients with B cell non-Hodgkin′s lymphoma(B-NHL),and to investigate the correlation between the level of these genes and the progression of B-NHL.Methods The expression of BAFF,BAFF-R and Bcl-xL in peripheral blood mononuclear cells(PBMC) from patients(n=36) and healthy volunteers(n=7) was examined via real-time fluorescence quantitative polymerase chain reaction(real-time q-PCR) at the mRNAs level.These data were further analyzed according to gender,lymphoma international prognostic index(IPI),age,clinical staging and drug resistance.Results The expressions of BAFF,BAFF-R and Bcl-xL were significantly increased in PBMC from patients with B-NHL compared with control group(P<0.05).The expression of these genes in groups of stage Ⅲ/Ⅳ and the resistant B-NHL was respectively higher than that of stageⅠ/Ⅱ and non resistant B-NHL groups(P<0.05).Conclusion The expression of BAFF,BAFF-R and Bcl-xL in PBMC from patients with resistant B-NHL is significantly increased,correlated with the B-NHL clinical staging.These results suggest that BAFF/BAFF-R mediated signal pathway is involved in the progression of B-NHL and drug resistance. |
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Keywords: | B cell non-Hodgkin′s lymphoma drug resistance neoplasm gene expression BAFF BAFF-R Bcl-xL B-cell activating factor receptor B-cell activating factor |
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