| 以CpG ODN为靶点应用生物传感器技术筛选抗炎中药 |
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| 引用本文： | 程娟,郑江,周红,蒋栋能,吴翀,陈益国,张向武,姚婕.以CpG ODN为靶点应用生物传感器技术筛选抗炎中药[J].中国临床药理学与治疗学,2005,10(11):1240-1244. |
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| 作者姓名： | 程娟 郑江 周红 蒋栋能 吴翀 陈益国 张向武 姚婕 |
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| 作者单位： | 1. 第三军医大学药理教研室重庆,400038
2. 西南医院中心实验室,重庆,400038 |
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| 摘 要： | 目的:筛选具有拮抗CpGODN作用的抗炎中药。方法:将CpGODN包被于生物传感器的生物素化样品池上,测定78种中药水煎液与CpGODN的亲和力;选择具有较高亲和力的中药水煎液,与CpGODN(33μg·ml-1)37℃孵育30min后,再次测定与CpGODN的亲和力。结果:78种中药中,地榆、大黄、藏青果等14种中药含有结合CpGODN的活性成分;鸦胆子、赤芍、地榆等9种中药活性成分含量较高。结论:筛选得到9种活性含量较高的中药,为下一步分离提纯有效成分单体提供了实验依据。
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| 关 键 词： | 生物传感器 中药 CpG ODN 亲和力 脓毒症 筛选 |
| 文章编号： | 1009-2501（2005）11-1240-05 |
| 收稿时间： | 2005-09-02 |
| 修稿时间： | 2005-10-11 |
Screening of anti-inflammatory Chinese traditional herbs targeted CpG ODN using biosensor technology |
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| CHENG Juan,ZHENG Jiang,ZHOU Hong,JIANG Dong-neng,WU Chong,CHEN Yi-guo,ZHANG Xiang-wu,YAO Jie.Screening of anti-inflammatory Chinese traditional herbs targeted CpG ODN using biosensor technology[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2005,10(11):1240-1244. |
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| Authors: | CHENG Juan ZHENG Jiang ZHOU Hong JIANG Dong-neng WU Chong CHEN Yi-guo ZHANG Xiang-wu YAO Jie |
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| Institution: | CHENG Juan,ZHENG Jiang1,ZHOU Hong,JIANG Dong-neng1,WU Chong,CHEN Yi-guo1,ZHANG Xiang-wu1,YAO Jie1 Department of Pharmacology,the Third Military Medical University,1 Medical Research Center,Southwestern Hospital,the Third Military Medical University,Chongqing 400038,China |
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| Abstract: | |
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| Keywords: | biosensor Chinese traditional medicine CpG ODN affinity sepsis screening |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
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