| 反相高效液相色谱法测定复方磺胺嘧啶分散片中磺胺嘧啶的含量 |
| |
| 引用本文： | 庹芹,郝新才.反相高效液相色谱法测定复方磺胺嘧啶分散片中磺胺嘧啶的含量[J].中国医院用药评价与分析,2013(7):626-628. |
| |
| 作者姓名： | 庹芹 郝新才 |
| |
| 作者单位： | [1]十堰市妇幼保健院药剂科,湖北十堰442000 [2]湖北医药学院附属太和医院药学部,湖北十堰442000 |
| |
| 摘 要： | 目的：建立反相高效液相色谱法（RP-HPLC）测定复方磺胺嘧啶分散片中磺胺嘧啶的含量。方法：采用C18（200 mm×4.6 mm,5μm）色谱柱;流动相：0.05 mol/L磷酸二氢钾溶液-甲醇（60∶40）;检测波长：242 nm;柱温：30℃。结果：磺胺嘧啶进样量在10～90μg/ml范围内与峰面积呈良好的线性关系（r=0.999 7）;磺胺嘧啶平均加样回收率为99.9%,RSD为2.7%。结论：该方法准确、简便,可用于复方磺胺嘧啶分散片中磺胺嘧啶含量的测定。
|
| 关 键 词： | 复方磺胺嘧啶分散片 含量测定 反相高效液相色谱法 |
Determination of Sulfadiazine in Compound Sulfadiazine Dispersible Tablets by HPLC |
| |
| TUO Qin,HAO Xin-cai.Determination of Sulfadiazine in Compound Sulfadiazine Dispersible Tablets by HPLC[J].Evaluation and Analysis of Drug-Use in Hospital of China,2013(7):626-628. |
| |
| Authors: | TUO Qin HAO Xin-cai |
| |
| Institution: | 1. Dept. of Pharmacy, Shiyan Maternal and Child Health Hospital, Hubei Shiyan 442000; 2. Dept. of Pharmacy, Taihe Hospital Affiliated to Hubei University of Medicine, Hubei Shiyan 442000, China) |
| |
| Abstract: | OBJECTIVE：To establish HPLC method for determination of sulfadiazine in compound sulfadiazine dispersible tablets. METHODS： The separation of samples was performed on C18（200 mm × 4.6 mm, 5μm） chromatographic column with mobile phase consisting of potassium 0.05 mol/L potassium dihydrogen phosphate solution and methanol （60： 40） with detection wavelength of 242 nm and column temperature of 30℃. RESULTS： The optimal linear range of schisandrin was 10-90 μg/ml（ r = 0. 999 7 ） with average recovery of 99.9% （RSD = 2. 7% ）. CONCLUSION： The method is accurate and simple, and it is applicable for determination of sulfadiazine in compound sulfadiazine dispersible tablets. |
| |
| Keywords: | Compound sulfadiazine dispersible tablets Determination RP-HPLC |
| 本文献已被 维普 等数据库收录！ |
