元胡止痛片中延胡索乙素和欧前胡素的含量测定

目的：建立高效液相色谱法同时测定元胡止痛片中两种功效成分延胡索乙素和欧前胡素的含量。方法：色谱柱为AKZONOBEL Kromasil 100-5 C18柱（250 mm×4.6 mm,5μm）;流动相为甲醇-0.1%磷酸溶液（52∶48）混合后以三乙胺调pH至6.5;流速1.0 ml·min-1;柱温40℃;检测波长284 nm;进样量10μl。结果：延胡索乙素在0.004 2~0.084 0 mg·ml-1范围线性关系良好,r=1.000 0,平均回收率97.72%,RSD为1.10%（n=6）;欧前胡素在0.003 6~0.072 0 mg·ml-1范围线性关系良好,r=0.999 7,平均回收率为97.99%,RSD为0.96%（n=6）。结论：本方法操作简便,准确,可用于完善元胡止痛片的质量标准。

Content Determination of Tetrahydropalmatine and Imperatorin in Yuanhu Zhitong Tablets

Objective： To establish a method for the content determination of tetrahydropalmatine and imperatorin in Yuanhu Zhitong tablets by HPLC. Methods： An AKZONOBEL Kromasil 100-5 C18column（ 250 mm × 4. 6 mm,5 μm） was used and the mobile phase consisted of methanol and 0. 1% phosphoric acid（ 52∶ 48）（ adjusting pH to 6. 5 with triethylamine）,the flow rate was 1. 0 ml·min- 1at the column temperature of 40℃,the detection wavelength was at 284 nm,and the injection volume was 10 μl. Results： The linear range of tetrahydropalmatine was 0. 004 2-0. 084 0 mg·ml- 1（ r =1.000 0）. The average recovery was 97.72% with RSD of1. 10%（ n = 6）. The linear range of imperatorin was 0. 003 6-0. 072 0 mg·ml- 1（ r =0.999 7）. The average recovery was 97.99%with RSD of 0. 96%（ n = 6）. The measurement results of 10 batches were stable and reliable. Conclusion： The method is simple and accurate,which can be used to improve the quality standard.