小儿遗尿分散片中α-细辛醚、β-细辛醚的含量测定

目的：建立小儿遗尿分散片中α-细辛醚、β-细辛醚的含量测定方法.方法：采用反相高效液相色谱法.色谱柱：Hypersil ODS C18柱（250 mm×4.6 mm,5 μm）;流动相：甲醇∶水（60∶40）;检测波长：257 nm;柱温：30℃;流速：1.0 ml·min-1.结果：α-细辛醚在0.051～1.286 mg·ml-1范围内与峰面积线性关系良好,r=0.999 6,平均加样回收率为101.48%,RSD为1.07%;β-细辛醚在0.187～4.672 mg·ml-1范围内与峰面积线性关系良好,r=0.999 9,平均加样回收率为99.65%,RSD为1.31%.结论：所建立的方法简便、准确,可作为小儿遗尿分散片的质量控制方法.

Determination of α-asarone and β-asarone in Children Yiniao Dispersible Tablets

T Objective： To establish the method for determination of α-asarone and βasarone in children＇ s yiniao dispersible tab- lets. Method： RP-HPLC with Hypersil ODS Cls（250 mm ×4.6 mm,5 μm）column was applied with methanol： water （60： 40） as the mobile phase, detection wavelength at 257 nm, column temperature at 30 ℃ and the flow rate was 1.0 ml-min-1. Result： The linear range of calibration curve was 0. 051-1. 286 mg·ml-1 for α-asarone （r =0. 999 6）. The average recovery was 101.48% and RSD was 1.07%. The linear range of calibration curve was 0. 187- 4. 672 mg· ml-1 for β-asarone （r = 0.999 9）. The average recovery was 99. 65% and RSD was 1.31%. Conclusion： The method is simple and accurate, and can be used as a quality control method for children yiniao dispersible tablets.