| 高效液相色谱法测定炎可宁中大黄素和大黄酚含量 |
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| 引用本文： | 林远风,黄燕萍.高效液相色谱法测定炎可宁中大黄素和大黄酚含量[J].中国药业,2009,18(8):25-26. |
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| 作者姓名： | 林远风 黄燕萍 |
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| 作者单位： | 1. 广西壮族自治区北海市人民医院,广西,北海,536000
2. 广西壮族自治区北海食品药品检验所,广西,北海,536000 |
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| 摘 要： | 目的采用高效液相色谱（HPLC）法测定炎可宁片中大黄素和大黄酚的含量。方法色谱柱为Shimadzu C18柱（250mm×4．6mm，5μm），流动相为甲醇-0．1％磷酸溶液（85：15），流速为0．8mL／min，检测波长为254nm。结果大黄素、大黄酚与其相邻质峰能完全分离，大黄素与大黄酚质量浓度分别在0．586～29．300μg／mL（n=0．9999）和1．581～79．050μg／mL（r2=1．0000）范围内与峰面积线性关系良好，平均回收率分别为99．58％和100．40％，RSD分别为1．42％和1．82％（n=6）。结论HPLC法简便、准确、重现性好，能排除其他成分的干扰，可用于炎可宁的质量控制。
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| 关 键 词： | 高效液相色谱法 大黄素 大黄酚 炎可宁片 |
Determination of Emodin and Chrysophanol in Yankening Tablets by HPLC |
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| Lin Yuanfeng,Huang Yanping.Determination of Emodin and Chrysophanol in Yankening Tablets by HPLC[J].China Pharmaceuticals,2009,18(8):25-26. |
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| Authors: | Lin Yuanfeng Huang Yanping |
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| Institution: | 1. Beihai City People＇s Hospital, Beihai, Guangxi, China 536000; 2. Beihai Institute for Food and Drug Control, Beihai, Guangxi, China 536000) |
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| Abstract: | Objective To establish a HPLC method for the determination of emodin and chrysophanol in Yankening Tablets. Methods The Shimadzu C18 column （250 mm×4.6 mm,5μm）was used with the mobile phase of methanol-0. 1% H3PO4 （85：15） and the flow rate was 0. 8 mL/min. The detection wavelength was at 254 nm. Emodin and chrysophanol could be completely separated from adjacent impurity peaks. Results The linear range of emodin was between 0. 586 - 29. 300 μg/mL, r1 = 0. 999 9; and the linear range of chrysophanol was be- tween 1. 581 - 79. 050 μg/mL, r2 = 1. 0000. The average recovery rate of emodin and chrysophanol was 99.58% with RSD 1.42%, 100.40% with RSD 1.82% （n =6）, respectively. Conclusion The methods is simple, accurate with good reproducibility. It can be used for the quality control of Yankening Tablets. |
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| Keywords: | HPLC emodin chrysophanol Yankening Tablets |
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