| 刺五加及其注射液中槲皮素的含量测定 |
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| 引用本文： | 陈勇川,宋伟,刘松青.刺五加及其注射液中槲皮素的含量测定[J].中国药房,2002,13(3):165-166. |
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| 作者姓名： | 陈勇川 宋伟 刘松青 |
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| 作者单位： | 第三军医大学西南医院药剂科,重庆市,400038 |
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| 摘 要： | 目的 :建立高效毛细管电泳法测定刺五加和刺五加注射液中槲皮素的含量方法。方法 :运行缓冲液为50mmol/L硼酸 -50mmol/L十二烷基磺酸钠缓冲液 ,pH值为8 5 ,操作电压为20kV ,检测波长为260nm。结果 :测定槲皮素的线性范围为0 0125～1 0mg/ml ,r=0 9997 ;刺五加的加样回收率为 (98 99±0 63) % ,刺五加注射液的加样回收率为 (98 62±0 42) %。结论 :本分析方法简便、快速、准确 ,适合刺五加及刺五加注射液中槲皮素的含量测定
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| 关 键 词： | 高效毛细管电泳法 刺五加 刺五加注射液 槲皮素 |
| 文章编号： | 1001-0408(2002)03-0165-02 |
| 修稿时间： | 2001年4月2日 |
Determination of Quercetin in Acanthopanax and Acanthopanax Injection by High Performance Capillary Electrophore-sis(HPCE) |
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| CHEN Yongchuan,SONG Wei,LIU Songqing.Determination of Quercetin in Acanthopanax and Acanthopanax Injection by High Performance Capillary Electrophore-sis(HPCE)[J].China Pharmacy,2002,13(3):165-166. |
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| Authors: | CHEN Yongchuan SONG Wei LIU Songqing |
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| Abstract: | OBJECTIVE:A HPCE method for determination of Quercetin in acanthopanax and acanthopanax injection was developed.METHODS:Measurements were carried out in a buffer solution containing 50mmol/L boric acid solution and 50mmol/L SDS(pH 8.5),at 20kV running voltage applied to a 50cm×50μm capillary.The detection wavelength was 260nm.RESULTS:The linear range of quercetin was 0.0 125～1.0mg/ml.The recovery of acanthopanax was (98.99±0.63)%.The recovery of acanthopanax injection was (98.62±0.42)%.CONCLUSION:The method is simple,rapid,accurate and suitable for determination of quercetin in acanthopanax and acanthopanax injection. |
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| Keywords: | HPCE acanthopanax acanthopanax injection quercetin |
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