牛蒡根4种提取物体外抗肿瘤活性的比较研究

目的:研究牛蒡根4种提取物体外抗肿瘤活性。方法:以系统溶剂分离法分别提取牛蒡根的石油醚、氯仿、乙酸乙酯、正丁醇部位。采用MTT法检测4种提取物对人乳腺癌细胞MCF-7、人胃腺癌细胞BGC-823、小鼠肝癌细胞HepA、肉瘤细胞S180和小鼠脾淋巴细胞生长的抑制作用。结果:牛蒡根石油醚、氯仿、乙酸乙酯3种提取物对MCF-7细胞和BGC-823细胞在体外有一定生长抑制作用;牛蒡根4种提取物对HepA细胞、S180细胞和小鼠脾淋巴细胞在体外有一定的生长抑制作用。结论:牛蒡根石油醚、氯仿、乙酸乙酯提取物体外具有一定的抗肿瘤活性,这种作用与增强细胞免疫活性无关。

Study on Anti-tumor Activities of 4 Different Extracts from the Root of Arctium lappa

OBJECTIVE：To investigate the anti-tumor activities of 4 different extracts from Arctium lappa root.METHODS：The parts of petroleum ether,chloroform,acetic ether and n-butanol from the root of A.lappa were extracted by systematic solvent method.MTT method was employed to evaluate the inhibitory effect of 4 extracts on the proliferation of mouse hepatoma carcinoma cells HepA and sarcoma cells S180,human breast carcinoma cells MCF-7,gastric adenocarcinoma cells BGC-823 and mice spleen lymphocytes.RESULTS：The petroleum ether,chloroform and acetic ether extracts of A.lappa root inhibited the proliferation of tumor cells MCF-7 and BGC-823.4 extracts inhibited the proliferation of HepA cells,S180 cells,mice spleen lymphocytes.CONCLUSIONS：A.lappa root extracts have antitumor effects in vitro and the activities don＇t correlate with the cellular immunity.