加压氧对阿糖胞苷体外诱导急性髓性白血病细胞凋亡的影响

①目的　用体外实验探讨加压氧 (HBO)对阿糖胞苷 (Ara c)诱导急性髓性白血病 (AML)细胞凋亡的影响。②方法　在AML病人的单个核细胞培养体系中加入Ara c,观察经HBO处理和未经HBO处理两组凋亡细胞形态 ,并测定各组细胞活性、Bax/Bcl 2积分比值和原位细胞凋亡 (TUNEL)阳性率。③结果　药物加HBO组具有典型凋亡特征的AML细胞活性明显低于药物未经HBO处理组 (F =7.75 ,q =2 .4 1,P <0 .0 5 ) ;Bax/Bcl 2积分比值明显高于未经HBO处理组 (F =10 .0 6 ,q =3.97,P <0 .0 5 ) ;TUNEL阳性率明显高于未经HBO处理组 (F =30 .79,q =4 .4 4 ,P <0 .0 1)。④结论　HBO增强了AML细胞对阿糖胞苷的敏感性 ,使得阿糖胞苷体外诱导AML细胞凋亡增加。

INFLUENCE OF HYPERBARIC OXYGEN ON CELL APOPTOSIS OF Ara-c INDUCED ACUTE MYELOID LEUKEMIA: AN IN-VITRO STUDY

Objective\ To investigate the influence of hyperbaric oxygen(HBO) on the cell apoptosis in vitro of Ara cinduced Acute myeloid leukemia(AML).\ Methods\ Ara c was added to mononuclear cells of AML patients .The morphological appearance of apoptotic cells was investigated for HBO treated Group and Non HBO treated Group. Cell activity,Bax/Bcl 2 ratio and TUNEL positive rate of two groups were detected. \ Results\ The cell activity with typical feature of AML for HBO treated Group was lower than that for Non HBO treated Group ( F=7.75, q=2.41,P <0.05); Bax/Bcl 2 ratio and TUNEL positive rate were higher than those for Non HBO treated Group ( F=10.06,q=3.97, P <0.05 and F=30.79, q=4.44, P <0.01, respectively). \ Conclusion\ HBO enhances the sensitivity of AML cells to Ara c in vitro and increases cell apoptosis of Ara c induced AML.