| 全反式维甲酸对兔颈动脉粥样硬化病灶VSMC增殖及AKT1表达影响 |
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| 引用本文： | 张青青,董果雄,张社华.全反式维甲酸对兔颈动脉粥样硬化病灶VSMC增殖及AKT1表达影响[J].青岛大学医学院学报,2010,46(2):135-137,139. |
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| 作者姓名： | 张青青 董果雄 张社华 |
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| 作者单位： | 1. 青岛大学医学院,附属医院心内科,山东,青岛,266003
2. 青岛大学医学院,实验中心,山东,青岛,266003 |
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| 摘 要： | 目的探讨全反式维甲酸(atRA)对兔颈动脉内膜损伤后内膜增生以及蛋白激酶B(AKT1)、增殖细胞核抗原(PCNA)表达的影响和机制。方法新西兰雄性大白兔36只,随机分为6组:假手术A、B组,手术A、B组,手术并予atRA治疗A、B组(治疗A、B组),每组各6只。各组均给予高脂饮食14d后,手术组作颈动脉内膜损伤模型。治疗组于术前3d开始每天给予atRA灌胃,其余操作同手术组。假手术组手术但不损伤内膜。于术后7d处死A组动物,28d处死B组动物。采取颈动脉标本,对血管粥样硬化病变进行大体形态学观察,用免疫组化法检测粥样硬化病灶中AKT1、PCNA的表达水平。结果手术组术后7d内膜开始增生,28d时增生明显,粥样斑块形成,管腔狭窄,AKT1、PCNA阳性表达明显。治疗组内膜增生较轻,28d时,内膜面积及斑块厚度低于手术组(t=3.797、3.704,P<0.01),AKT1、PCNA阳性表达指数亦低于手术组(t=4.741、2.890,P<0.05)。结论atRA能抑制兔颈动脉内皮损伤后AKT1、PCNA的表达,抑制平滑肌细胞增殖,从而抑制内膜增生及动脉粥样硬化病变进程。
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| 关 键 词： | 维甲酸 肌细胞 平滑肌 冠状动脉再狭窄 原癌基因蛋白质c-akt |
EFFECT OF ALL-TRANS RETINOIC ACID ON PROLIFERATION OF VASCULAR SMOOTH MUSCLE CELLS AND THE EXPRESSION OF AKT1 IN RABBIT CAROTID ATHEROSCLEROSIS LESIONS |
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| ZHANG QING-QING,DONG GUO-XIONG,ZHANG SHE-HUA.EFFECT OF ALL-TRANS RETINOIC ACID ON PROLIFERATION OF VASCULAR SMOOTH MUSCLE CELLS AND THE EXPRESSION OF AKT1 IN RABBIT CAROTID ATHEROSCLEROSIS LESIONS[J].Acta Academiae Medicinae Qingdao Universitatis,2010,46(2):135-137,139. |
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| Authors: | ZHANG QING-QING DONG GUO-XIONG ZHANG SHE-HUA |
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| Institution: | Department of Cardiology;The Affiliated Hospital of Qingdao University Medical College;Qingdao 266003;China |
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| Abstract: | Objective To investigate the influence of all-trans retinoic acid (atRA) on intima proliferation and expressions of AKT1 and PCNA in rabbit carotid artery after endomembrane injury.MethodsThirty-six New Zealand rabbits wereevenly randomized to six groups:sham-operation group A and group B; operation group A and group B; and treatment group A and group B. All the rabbits were fed with high fat diet for 14 days. A model of carotid artery endomembrane injury was created in operation group. In treatment groups,... |
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| Keywords: | tretinoin myoblasts smooth muscle coronary restenosis proto-oncogene proteins c-akt |
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