| 积雪草甙对增生性瘢痕成纤维细胞增殖与Smad7蛋白的影响 |
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| 引用本文： | 潘姝,利天增,李叶扬,陈晓东,谢举临,徐盈斌,祁少海.积雪草甙对增生性瘢痕成纤维细胞增殖与Smad7蛋白的影响[J].第三军医大学学报,2005,27(1):49-52. |
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| 作者姓名： | 潘姝 利天增 李叶扬 陈晓东 谢举临 徐盈斌 祁少海 |
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| 作者单位： | 中山大学附属第一医院烧伤科,广州,510080;广州市红十字会医院烧伤整形科,广州,510270 |
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| 摘 要： | 目的研究积雪草甙对增生性瘢痕成纤维细胞增殖及对Smad7蛋白的影响.方法用组织块法培养人瘢痕成纤维细胞.应用流式细胞术、免疫细胞化学、RT-PCR及Western blot技术结合光密度扫描分析,观察积雪草甙对增生性瘢痕成纤维细胞细胞周期、凋亡和Smad7在细胞中的定位及其mRNA和蛋白含量的改变.结果积雪草甙可抑制瘢痕的成纤维细胞从S期进入M期,增加瘢痕成纤维细胞中Smad7 mRNA和蛋白的含量.结论积雪草甙抑制瘢痕的作用可能部分通过增加抑制性Smad7蛋白.
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| 关 键 词： | 积雪草甙 增生性瘢痕 Smad7 |
| 文章编号： | 1000-5404(2005)01-0049-04 |
| 修稿时间： | 2004年5月6日 |
Effects of asiaticoside on cell proliferation and Smad7 expression of hypertrophic scar fibroblasts |
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| PAN Shu ,LI Tian-zeng ,LI Ye-yang ,CHEN Xiao-dong ,XIE Ju-lin ,XU Ying-bin ,QI Shao-hai.Effects of asiaticoside on cell proliferation and Smad7 expression of hypertrophic scar fibroblasts[J].Acta Academiae Medicinae Militaris Tertiae,2005,27(1):49-52. |
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| Authors: | PAN Shu LI Tian-zeng LI Ye-yang CHEN Xiao-dong XIE Ju-lin XU Ying-bin QI Shao-hai |
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| Institution: | PAN Shu 1,LI Tian-zeng 1,LI Ye-yang 2,CHEN Xiao-dong 1,XIE Ju-lin 1,XU Ying-bin 1,QI Shao-hai 1 |
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| Abstract: | Objective To investigate the effects of asiaticoside on the proliferation and expression of Smad7 of hypertrophic scar fibroblasts. Methods The hypertrophic scar fibroblasts were cultured with tissue culture method. Flow cytometry, immunocytochemistry, RT-PCR, and Western blot were applied to investigate the cell cycle, cell apoptosis, and expression of Smad7 with or without asiaticoside. Results Asiaticoside could inhibit hypertrophic scar fibroblasts entering from phase S to phase M and increase the protein contents and Smad7 mRNA in hypertrophic scar fibroblasts. Conclusion Asiaticoside may inhibit the formation of hypertrophic scar partly through the increase of Smad7 expression. |
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| Keywords: | Smad7 |
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