参附注射液对大鼠缺血再灌注心肌Bcl-2、Bax与c-fos基因蛋白表达的影响

目的 观察大鼠急性缺血再灌注损伤与凋亡相关基因Bcl-2、c-fos和Bax蛋白表达的关系及参附注射液(SF)的影响.方法 采用整体缺血再灌注(ischemia-reperfusion,IR)模型,35只大鼠分为假手术组(C组)、生理盐水30 min对照组(IR 30)、生理盐水120 min对照组(IR 120)、SF 30 min组(SF 30)和SF 120 min组(SF 120),心肌缺血40 min再灌注30 min或120 min后,用免疫组化法检测Bcl-2、Bax和c-fos在心肌中的表达量.结果 与C组比较,IR组心肌Bcl-2、Bax和c-fos蛋白表达显著升高(P＜0.01),Bcl-2/Bax比值显著下降(P＜0.01);与IR组比较,SF组Bcl-2蛋白表达显著升高(P＜0.01),Bax和c-fos蛋白表达显著降低(P＜0.01),Bcl-2/Bax比值显著升高(P＜0.01).结论 SF对IR心肌保护效应可能与其促进Bcl-2蛋白高表达、抑制Bax与c-fos蛋白表达、增加Bcl-2/Bax比值,从而抑制心肌细胞凋亡有关.

Effect of Shenfu parenteral injection on Bcl-2, Bax and c-fos proteins expression in rat ischemia-reperfusion myocardium

Objective To observe the relationship between acute ischemia-reperfusion injury and apoptosis related gene Bcl-2 and Bax and c-fos protein expression,and the intervention of Shenfu parenteral injection(SF) in rats.Methods The ischemia-reperfusion(IR) model was established by ligating the left anterior descending coronary artery.Thirty-five healthy rats were randomly divided into sham operation group(group C),IR group and SF group(8 ml/kg SF was intravenously injected 15 min before ligation).After myocardial ischemia lasting for 40 min,the myocardium was reperfused for 30 min or 120 min,then the expression of Bcl-2 and Bax and c-fos proteins was detected in myocardium by immunohistochemistry.Results As compared with group C,the protein expression of Bcl-2 and Bax and c-fos in the ischemic myocardium significantly increased(P<0.01),and Bcl-2/Bax ratio significantly decreased(P<0.01) in IR group.As compared with IR group,the protein expression of Bcl-2 significantly increased(P<0.01),and Bax and c-fos significantly decreased(P<0.01),Bcl-2/Bax ratio significantly increased(P<0.01) in SF group.Conclusion Protective effect of SF on ischemia-reperfusion myocardium may concern with restraining myocardial cell apoptosis through promoting Bcl-2 protein expression and decreasing Bax and c-fos protein expression as well as increasing Bcl-2/Bax ratio.