肉桂醛促进子宫内膜癌RL95-2细胞凋亡的机制

【目的】探讨肉桂醛对子宫内膜癌RL95-2细胞凋亡的影响及其作用机制。【方法】选取肉桂醛作用子宫内膜癌RL95-2细胞，噻唑蓝（MTT）比色法检测子宫内膜癌RL95-2细胞的增殖活性和肉桂醛的IC50，Hoechst33258染色荧光显微镜观察细胞凋亡形态学改变，流式细胞术检测RL95-2细胞的凋亡百分率，Western blot检测肉桂醛对RL95-2细胞Cleavedcaspase-3、Caspase-3、NF-κB、IL-6和IGF-R蛋白表达的影响。【结果】肉桂醛可降低子宫内膜癌RL95-2细胞的增殖率，与药物浓度和作用时间有关；与溶剂对照组比较，肉桂醛组（0.29、0.59和1.20mg/mL）作用48h后RL95-2细胞的凋亡百分率明显增高（P<0.01），出现典型的凋亡小体，Cleavedcaspase-3蛋白的表达明显增加（P<0.01），Caspase-3蛋白的表达无明显变化（P>0.05），而NF-κBp65、IL-6和IGF-R蛋白的表达均明显降低（P<0.05）。【结论】肉桂醛可降低RL95-2细胞NF-κB·p65、IL-6和IGF-R蛋白的表达，促进RL95-2细胞的凋亡，从而起到抗子宫内膜癌作用。

Mechanism of Cinnamaldehyde Promoting the Apoptosis of RL95-2 Cells in Endometrial Carcinoma

【Objective】To investigate the effect of cinnamaldehyde on the apoptosis of RL95- 2 cell in endometrial carcinoma. 【Methods】 Endometrial carcinoma RL95- 2 cells were treated with cinnamaldehyde，and the proliferation activity and IC50 of endometrial carcinoma RL95-2 cells were detected by MTT colorimetry assay. Apoptotic morphology was observed after Hoechst 33258 staining. The percentage of apoptosis in RL95-2 cells was measured by flow cytometry. Western blot analysis was used to test the effect of cinnamaldehyde on the expression of Cleaved caspase- 3，caspase-3， NF-κB·p65，IL-6 and IGF-R in RL95-2 cells.【Results】Cinnamaldehyde can reduce the viability rate of endometrial cancer RL95- 2 cells，which is related to the treatment duration and concentration. Compared with the solvent control group， the apoptosis percentage of RL95- 2 cells in the cinnamaldehyde group （0.29， 0.59， 1.20 mg/mL） was significantly increased after 48 hours（P < 0.01），typical apoptotic bodies were found ，and the expression of Cleaved caspase-3 protein was significantly increased（P < 0.01），there was no significant change in the expression of Caspase 3 protein（P > 0.05），while the expression of NF- κB · p65，IL- 6 and IGF- R protein were significantly increased（P <0.05）.【Conclusion】Cinnamaldehyde can reduce the expression of NF-κB·p65，IL-6 and IGF-R proteins in RL95-2 cells and promote the apoptosis of RL95-2 cells，thus playing an anti-endometrial cancer role.