山茱萸不同栽培品种的RAPD分析

目的分析山茱萸不同栽培品种之间的遗传关系,为山茱萸品种选育提供依据。方法采用改良后的CTAB法从山茱萸叶片中提取总DNA,进行RAPD分析;采用SPSS10.0软件计算DICE遗传相似性系数,Be-tween-groupslinkage方法聚类,构建亲缘关系树系图。结果共筛选出22个10个碱基随机引物用于PCR扩增,12个样品获得了133条带,其中多态性条带75条,占56.39%。聚类分析结果表明纺锤形果型自成一类,短梨形果型与短圆柱形果型聚为一类,其余几种较长大的果型聚为一类,反映了栽培过程中人为选择的后果。结论实验结果与山茱萸栽培品种的生物特性和区域分布基本一致;RAPD分析可作为对山茱萸品种选育的辅助手段。

RAPD Analysis on different cultivars of Cornus officinalis

Objective To analyze the genetic relationship among the different cultivars of Cornus officinalis and provide some foundation for heredity breeding. Methods A modified method of extracting total DNA from the leaves of C. officinalis was selected by improving the traditional method-CTAB, the total DNA was analyzed by RAPD; the genetic similarity correlation was calculated by SPSS 10.0 DICE method, cluster analyses were carried out using Between-groups linkage method, and the genetic dendrogram was established. Results Random primers (22 10-bp) were selected to be used for the PCR, a total of 133 bands were amplified by 12 samples, among which 75 bands were polymorphic, accounting for 56.39%. The cluster analysis indicated that: spindleform itself was one group, short pear-shape and short cylindericform clustered together, the rest longer types clustered together, which reflected the result of artificial selection in the course of cultivating. Conclusion The result keeps accordance with the biologic character and territorial distribution of C. officinalis cultivars; RAPD analysis is an assistant mean for seed breeding of C. officinalis.