改良方法处理的生物瓣膜组织宿主内皮化的实验研究

目的：探讨L－谷氨酸（L－G）改良方法处理的异种生物瓣膜组织寄主内皮化的可行性。方法：将戊二醛或戊二醛和L－谷氨酸处理的2组牛心包进行体外内皮细胞种植培养，并同时将此2组牛心包植入猪的右心房，观察心包片宿主内皮细胞生长的形态学特征。结果：组织学及扫描电镜分析显示，改良L－谷氨酸组牛心包体外内皮细胞种植培养及动物心脏内移植后，心包组织无钙化，心包表面内皮细胞生长良好，而戊二醛组牛心包钙化及胶原纤维断裂明显，未见内皮细胞生长，结论：采用改良L－谷氨酸处理方法可有效地抑制常规戊二醛处理生物瓣膜组织所残留的细胞毒性，有助于宿主内皮细胞在生物瓣膜上的生长，优化生物瓣膜的生化处理，减少生物瓣膜的钙化，衰坏并改善其生物相容性。

Experimental host endothelialization of bioprosthetic heart valves treated by modified biochemical method

Objective To test the feasibility of a modified glutaraldehyde-preserved procedure for treating bovine pericardium material according to its host endothelialization. Methods Bovine pericardium flaps, separately treated with 0.5% glutaraldehyde alone and combined glutaraldehyde and 8%L-glutamic acid, were implanted in the culture of human endothelial cells from umbilical vein for in vitro study. Simultaneous in vivo study was carried out by transplanting the pericardium flaps treated both ways into the pig right atrium. Morphologic analysis of the implanted bovine pericardium flaps was made 16 days later. Results Morphological analysis demonstrated that the growth of endothelial cells was satisfactory on the biologic tissue treated with glutaraldehyde plus L-glutamic acid, both in vitro and in vivo. While in the controls, no growth of host endothelial cells was noticed, and instead, severe calcification and rupture of collagen fibrils were detected. Conclusion L-glutamic acid can efficiently minimize the toxicity of residual aldehyde and accelerate the growth of host endothelial cells, thus optimizing the biochemical procedure to treat biologic heart valves, inhibiting the calcification and degeneration and improving the biocompatibility of the biological heart valve.