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兔晶状体中钙调素的定位研究
引用本文:张明志,董红艳.兔晶状体中钙调素的定位研究[J].徐州医学院学报,1990,10(2):143-146.
作者姓名:张明志  董红艳
作者单位:徐州医学院生物化学研究室 (张明志,张光毅,赵昇皓),徐州医学院电镜室 (董红艳,陈世超,赵强),徐州医学院电镜室(于宏丽)
摘    要:

关 键 词:钙调素  晶状体  免疫组化  定位

LOCALIZATION OF CALMODULIN IN RABBIT LENS
Zhang Mingzhi.LOCALIZATION OF CALMODULIN IN RABBIT LENS[J].Acta Academiae Medicinae Xuzhou,1990,10(2):143-146.
Authors:Zhang Mingzhi
Abstract:Calmodulin (CaM) which had been shown to be homogeneons by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) was modified by performic acid and the rabbits were immunized with it. Localization of CaM in rabbit lens was studied with Protein-A gold method. In lens, from cndothlial cells to fibrous type cells in the cores, CaM content increased gradually. In endothelial cells, CaM mainly localized in mitochondria, microfilaments and nuclear. In the cortex, the localization of CaM in both fibrous type cells with or without nuclear was even and CaM content in the latter was greater. In the core, CaM was also evenly distributed.
Keywords:calmodulin  lens  hlstochemistry  electromicroscope  rabbit
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