联合放化疗对鼻咽癌患者CD4＋/CD25＋与CD28＋/TCR＋T淋巴细胞亚群的影响

目的：研究鼻咽癌患者联合放化疗前后CD4＋/CD25＋与CD28＋/TCR＋T淋巴细胞亚群的变化。方法：招募43例接受联合放化疗的鼻咽癌患者,在联合放化疗前、联合放化疗3周及6周后分别抽取外周静脉血,运用葡聚糖-泛影葡胺密度梯度离心法分离并收集淋巴细胞,流式细胞仪检测CD4＋/CD25＋与CD28＋/TCR＋T淋巴细胞亚群的比例。结果：联合放化疗3周及6周后CD4＋/CD25＋淋巴亚群无明显变化（P〉0.05）,CD28＋/TCR＋淋巴亚群明显升高（P〈0.05）。结论：联合放化疗可部分恢复鼻咽癌患者受抑制的免疫功能。

EFFECT OF CHEMO-RADIOTHERAPY ON CD4+/CD25+ AND CD28+/TCR+ T CELLS IN PATIENTS WITH NASOPHARYNGEAL CARCINOMA

Objective：To examine the effect of chemo-radiotherapy on CD4＋/CD25＋ and CD28＋ /TCR＋ T cells in peripheral blood in the patients with nasopharyngeal carcinoma （NPC）. Methods： Forty-three NPC patients were recruited and the peripheral venous blood samples were harvested before, and 3 and 6 weeks after the chemo-radiotherapy, respectively. The peripheral lymphocytes were isolated and collected by fi coll-hypaque density gradient centrifugation, and the proportions of CD4＋/CD25＋ and CD28＋/TCR＋ T cells were determined by flow cytometry. Results： The proportion of CD4＋/CD25＋T cells was unaffected after 3 or 6 weeks of chemo-radiotherapy （ P 〉0.05）, whereas the proportion of CD28＋/TCR＋ T cells was increased significantly （ P 〈0.05）. Conclusion： Standard chemo-radiotherapy is able to partly restore the suppressed immune function in NPC patients.