| 北虫草不同部位超氧化物歧化酶(SOD)酶活力的比较 |
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| 引用本文： | 梁迪思,王飞,郑家概,农云军.北虫草不同部位超氧化物歧化酶(SOD)酶活力的比较[J].辽宁中医药大学学报,2012(2):183-184. |
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| 作者姓名： | 梁迪思 王飞 郑家概 农云军 |
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| 作者单位： | 中国广州分析测试中心广东省化学危害应急检测技术重点实验室 |
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| 摘 要： | 目的:对北虫草菌丝体、子座的超氧化物歧化酶(SOD)酶活力进行对比实验,在水提取和丙酮分级提取两种方法中选择效果更好的一种。方法:利用水、丙酮分别对北虫草不同部位的超氧化物歧化酶(SOD)进行提取,邻苯三酚自氧化法检测其酶活性。结果:北虫草菌丝体的SOD酶活性和蛋白含量均明显高于子座,并且丙酮分级提取的效果更优。结论:北虫草各部分的SOD酶活性和蛋白含量各不相同,不同提取方法效果也有差异。
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| 关 键 词： | 北虫草 超氧化物歧化酶 酶活力 |
Comparative Study on SOD Enzyme Vitality of Different Parts of Cordyceps Militaris(Fr)Link |
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| LIANG Di-si,WANG Fei,ZHENG Jia-gai,NONG Yun-jun.Comparative Study on SOD Enzyme Vitality of Different Parts of Cordyceps Militaris(Fr)Link[J].Journal of Liaoning University of Traditional Chinese Medicine,2012(2):183-184. |
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| Authors: | LIANG Di-si WANG Fei ZHENG Jia-gai NONG Yun-jun |
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| Institution: | (Guangdong Key Laboratory of Emergency Chemical Test,China National Analytical Center,Guangzhou 510070,Guangdong,China) |
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| Abstract: | Objective:The Superoxide Dismutase(SOD)enzyme activity is identified by different extractations of water and acetone to mycelium and stroma of Cordyceps militaris(Fr)Link.Method:The SOD in different parts of Cordyceps militaris(Fr)Link was extracted with water and acetone respectively.Its enzyme activity was determined with the pyrogallol autoxidation method.Result:The SOD enzyme activity and the content of protein in mycelium were obviously higher than that in stroma,and the effect of fractional extraction with acetone was better.Conclusion:The SOD enzyme activity and the content of protein in different parts of Cordyceps militaris(Fr)Link are different,and the effects of different extractions are various. |
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| Keywords: | Cordyceps militaris(Fr)Link SOD enzyme activity |
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