IRS-4和GCKR基因多态性与2型糖尿病的关联性研究

目的 研究IRS-4和GCKR基因多态性与黑龙江汉族2型糖尿病的关联性.方法 采用PCR-RFLP技术检测黑龙江汉族2型糖尿病组、正常对照组人群的IRS-4基因密码子879C/G和GCKR基因P446L多态性,并分析其基因型频率及等位基因频率.结果 2型糖尿病组IRS-4基因879C/G的GG、GC、CC基因型频率分别为0.538、0.404、0.058,G及C等位基因频率分别为0.740、0.260;正常对照组IRS-4基因879C/G的GG、GC、CC基因型频率分别为0.556、0.352、0.092,G及C等位基因频率分别为0.732、0.268;糖尿病组和正常对照组之间IRS-4基因879C/G基因型频率及等位基因频率差异无统计学意义(P＞0.05).2型糖尿病组GCKR基因P446L的PP、PL、LL基因型频率分别为0.212、0.596、0.192,P和L等位基因频率分别为0.510、0.490;正常对照组GCKR基因P446L的PP、PL、LL基因型频率分别为0.148、0.463、0.389,P和L等位基因频率分别为0.380、0.620;糖尿病组和正常对照组之间GCKR基因P446L基因型频率及等位基因频率差异有统计学意义(P＜0.01).结论 IRS-4基因879C/G多态性与黑龙江省汉族2型糖尿病的发生无相关性,GCKR基因P446L多态性与黑龙江省汉族2型糖尿病的发生有相关性.

Association between IRS-4 and GCKR gene polymorphism in patients with type-2 diabetic mellitus population

Objective To explore the relationship between IRS-4 and GCKR gene polymorphism in patients with diabetic mellitus in Mudanjiang. Methods PCR-RFLP method was used to measure the polymorphism of IRS-4 and GCKR. Genotype frequency and allele frequency were analysised. Results In the analysis of IRS-4, the frequency of genotype GG, GC and CC in the Han population was 0.538, 0.404, and 0.058, respectively. The frequency of G and C allele was 0.740 and 0.260, respectively. In the control healthy population, the frequency of genotype GG, GC and CC was 0.556, 0.352 and 0.092, respectively. The frequency of G and C allele was 0.732 and 0.268, respectively. Both of the genotype frequency and allele frequency were similar between the two populations （P0.05）. In the analysis of GCKR, the frequency of genotype PP, PL and LL was 0.212, 0.596 and 0.192, respectively. The frequency of P and L allele was 0.510 and 0.490, respectively. In the healthy control population, the frequency of genotype PP, PL and LL was 0.148, 0.463 and 0.389, respectively. The frequency of P and L allele was 0.380 and 0.620, respectively. Both of the genotype frequency and allele frequency were similar between the two populations （P0.01）. Conclusion Only the gene polymorphism of GCKR P446L is correlated with T2DM in the Mudanjiang Han population.