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| 人工寒潮促发大鼠脑卒中发病前脑血管内皮细胞粘附分子mRNA的变化 | |
| 引用本文： | 林健雯,施晓耕,解龙昌,刘春岭,黄如训.人工寒潮促发大鼠脑卒中发病前脑血管内皮细胞粘附分子mRNA的变化[J].南方医科大学学报,2008,28(11):1954-1959. |
| 作者姓名： | 林健雯 施晓耕 解龙昌 刘春岭 黄如训 |
| 作者单位： | 1. 中山大学附属第一医院神经科,广东,广州,510080 2. 广东省中医院神经内科,广东,广州510120 3. 山西医科大学附属第一医院神经科,山西,太原,030000 |
| 基金项目： | 国家自然科学基金 |
| 摘 要： | 目的 探讨人工寒潮促发大鼠脑卒中发病前脑血管内皮细胞粘附分子mRNA的变化.方法 48只SD大鼠制成双肾双夹肾血管性高血压大鼠模型,分为寒潮和非寒潮两大组,再按血压水平各分为正常血压组、160～199mmHg、200-219 mmHg和≥220 mmHg等4个亚组,寒潮箱处理3 d后取嘴侧2 mm脑片进行VCAM-1、ICAM-1、P-选择素的RT-PCR检测;取视交叉平面连续切片,每8张取1张切片作原位杂交检测血管内皮的VCAM-1、ICAM-1、P-选择素的mRNA水平,取视交叉平面剩余切片及其余脑片连续切片,HE染色,了解是否有卒中病灶.发生脑卒中者被剔出卒中前状态的分析.结果 1)在正常血压、160～199 mmHg及200～219 mmHg血压亚组,寒潮组各级脑血管的VCAM-1、ICAM-1、P-选择素与GAPDH的比值及原位杂交阳性信号均比非寒潮组高;2)在≥220 mmHg血压亚组,寒潮组各级脑血管的上述指标均比非寒潮组降低;3)在非寒潮组,各级脑血管的上述指标均随血压升高而升高;在寒潮组,各级脑血管的上述指标也随血压升高而升高,然而在血压≥220mmHg时转为降低.结论 长期持续的高血压损害了脑血管内皮的调控功能,血压越高调控功能越差. |
| 关 键 词： | 人工寒潮 高血压 脑卒中 P-选择素 |
Changes in endothelial cellular adhesion molecule mRNA expressions in the cerebral blood vessels of rats with prestroke condition caused by simulated cold wave |
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| LIN Jian-wen,SHI Xiao-geng,XIE Long-chang,LIU Chun-ling,HUANG Ru-xun.Changes in endothelial cellular adhesion molecule mRNA expressions in the cerebral blood vessels of rats with prestroke condition caused by simulated cold wave[J].Journal of Southern Medical University,2008,28(11):1954-1959. | |
| Authors: | LIN Jian-wen SHI Xiao-geng XIE Long-chang LIU Chun-ling HUANG Ru-xun |
| Institution: | LIN Jian-wen1,SHI Xiao-geng2,XIE Long-chang3,LIU Chun-ling1,HUANG Ru-xun1 1Department of Neurology,First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China,2Department of Neurology,Guangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou 510120,3Department of Neurology,First Affiliated Hospital,Shanxi Medical University,Taiyuan 030001 |
| Abstract: | Objective To study the changes in the mRNA expression of endothelial cellular adhesion molecules in the cerebral blood vessels in rats with prestroke condition caused by simulated cold wave.Methods Two-kidney two-clip renovascular hypertension was induced in 48 male SD rats,which were subsequently randomly assigned into cold wave exposure and non-exposed group (n=24).Each group was further divided into 4 sub-groups according to their systolic blood pressure,namely the sham-operated group with blood pressure... |
| Keywords: | VCAM-1 ICAM-1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |