红景天苷对脓毒症小鼠脾脏免疫功能的影响

目的 评价红景天苷对脓毒症小鼠脾脏免疫细胞数量及功能的影响。方法 采用随机数字表法将30只C57BL小鼠分为假手术组、对照组和实验组（每组10只）。七氟烷麻醉下制备盲肠结扎穿孔脓毒症模型，手术后假手术组和对照组尾静脉给予1 mL生理盐水，实验组手术后尾静脉给予1 mL红景天苷（20 mg/kg）。各组小鼠尾静脉给药后12 h，取小鼠脾脏及外周血，ELISA检测细胞因子IL-6、IL-2和IL-10水平，流式细胞术检测小鼠脾脏CD4⁺与CD8⁺ T细胞数量变化，RT-PCR检测趋化因子CXCL-10 mRNA表达水平。结果 对照组较假手术组小鼠脾脏及外周血细胞因子IL-6，IL-2，IL-10水平显著升高；脾脏中CD4⁺与CD8⁺ T细胞数量显著增加；趋化因子CXCL-10 mRNA表达水平显著升高。与对照组相比，实验组小鼠脾脏及外周血细胞因子IL-6、IL-2、IL-10水平显著降低；脾脏中CD4⁺与CD8⁺ T细胞数量显著减少；CXCL-10 mRNA表达水平显著降低。结论 脓毒症时小鼠脾脏免疫功能发生明显变化，红景天苷可显著改善脓毒症小鼠脾脏免疫功能。

Effect of salidroside on immunologic function of spleen in sepsis mice

Objective To estimate the effects of salidroside on the amount of immune cells and immunological function of spleen in sepsis mice.Methods Thirty C57BL mice were equally divided into 3 groups (sham group, control group and experimental group) randomly and anesthesized with sevoflurance via cecal ligation and puncture. In sham group and control group, 1 mL of normal saline were applied in caudal vein, while the same volum of salidroside (20 mg/kg) was applied in the experimental group.Spleen and blood were harvested 12 h after injection, and centrifuged for measurement. Then expression of IL-6, IL-2 and IL-10 in spleen and plasma were measured via ELISA, the counts of CD4⁺ T and CD8⁺ T cells were measured via flow cytometry, and the expression of CXCL-10 mRNA was measured via RT-PCR.Results The expression of IL-6, IL-2 and IL-10 in spleen and plasma, the counts of CD4⁺ T and CD8⁺ T cells and CXCL-10 mRNA in spleen were all significantly higher in control group than those in sham group. More interestingly, we observed that salidroside significantly reduced the expression of cytokines including IL-6, IL-2, IL-10 as well as CD4⁺ T and CD8⁺ T cells count in spleen in the experimental group compared with the control group.In addition, salidroside also reduced the mRNA expression of CXCL-10.Conclusion The immune statue in spleen was changed significantly in sepsis. Salidroside could attenuate the immune function of spleen in sepsis.