通心痹合剂对动脉粥样硬化家兔血脂及炎性细胞因子的影响

目的:观察通心痹合剂对实验性动脉粥样硬化(atherosclerosis,AS)家兔血脂及炎症细胞因子水平的影响,探讨该方防治AS的机制。方法:将60只家兔随机分为空白组,模型组,辛伐他汀组,通心痹合剂大剂量组、中剂量组、小剂量组,每组10只。以球囊导管内皮损伤术和高脂饵食法建立家兔AS模型,通心痹合剂各剂量组及辛伐他汀组在造模的同时分别给药,空白组、模型组以等量生理盐水灌胃,8周后取出主动脉于光镜下进行病理观察,检测各组大鼠家兔血脂、白细胞介素-6(interleukin-6,IL-6)、IL-1、IL-10、肿瘤坏死因子-α(tumour necrosis factor alpha,TNF-α)。结果:病理结果显示,通心痹合剂大剂量组局部增厚的动脉内膜明显减轻,无泡沫细胞沉积及粥样斑块形成,优于辛伐他汀组;通心痹合剂各剂量组、辛伐他汀组均可明显降低家兔血脂、IL-6、IL-1、TNF-α水平,与模型组比较,差异均有统计学意义(P0.05,P0.01),但通心痹合剂大剂量组、中剂量组在降低LDL-C,升高HDL-C、IL-10方面与辛伐他汀组比较,差异有统计学意义(P0.05,P0.01)。结论:通心痹合剂对AS有明显的预防和治疗作用,调节血脂和降低炎症细胞因子水平可能是其作用机制之一。

Effect of Tongxinbi Mixture on the Blood Lipid and Inflammatory Cytokines of Atherosclerotic Rabbits

Objective:To observe the effect of Tongxinbi Mixture on serum lipids and inflammatory cytokines in experimental atherosclerosis (AS) rabbits and to explore the mechanism of Tongxinbi Mixture in prevention and treatment of atherosclerosis (AS).Methods:Sixty rabbits were randomly divided into blank group,model group,simvastatin group,high dose Tongxinbi Mixture group,middle dose Tongxinbi Mixture group and low dose Tongxinbi Mixture group,with 10 rats in each group.The AS model of rabbits was established with balloon catheter endothelium injury and high fat diet.The rats in Tongxinbi Mixture group and Simvastatin group were given the corresponding drugs,while the blank group and the model group were treated with equal volume of normal saline.After 8 weeks,the aorta was taken out and the pathology was observed under light microscope.The levels of serum lipids,interleukin-6 (IL-6),IL-1,IL-10,and tumor necrosis factor-α (TNF-α) of each group were detected.Results:The pathologic results showed that the thickening of arterial intima was notably reduced in the high dose Tongxinbi Mixture group and there was no foam cell deposition and atheromatous plaque formation were found,which were better than that of the Simvastatin group.There were significant decreases in levels of IL-6,IL-1 and TNF-α in all of the experimental groups compared with that of the model group (P ＜ 0.05,P ＜ 0.01).There were significant differences between the low dose group and the middle dose group(P ＜ 0.05,P ＜ 0.01) in the decrease of LDL-C and the increase of HDL-C and IL-10 of the simvastatin group.Conclusion:Tongxinbi Mixture has obvious preventive and therapeutic effects on AS and the mechanism may be the regulating of blood lipid and decreasing the level of inflammatory cytokines.