| ApoE^-/-；SAP—Tg小鼠模型的构建 |
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| 引用本文： | 吴腾,邢丽英,勾红菊,兰天,郑凌云,顾取良,温定文,王丽京.ApoE^-/-；SAP—Tg小鼠模型的构建[J].广东医药学院学报,2013(5):539-542. |
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| 作者姓名： | 吴腾 邢丽英 勾红菊 兰天 郑凌云 顾取良 温定文 王丽京 |
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| 作者单位： | [1]广东药学院,广东广州510006 [2]南方医科大学基础医学院,广东广州510515 [3]广东药学院基础学院,广东广州510006 |
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| 基金项目： | 国家自然科学基金（31271455;81200308;31100852;31200861） |
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| 摘 要： | 目的为研究SAP蛋白在动脉粥样硬化中的作用及机制，拟构建ApoE^-/-；SAP—Tg小鼠模型。方法分别选取6～8周龄的雄性ApoE^-/-小鼠与血清淀粉样蛋白P转基因（SAP—Tg）6周龄的雌性小鼠杂交，得到的杂交后代以剪尾方式提取基因组DNA，用PCR法检测小鼠的基因型。结果雄性ApoE^-/-小鼠与雌性SAP-Tg小鼠杂交得F1代后自交，在F2代获得ApoE^-/-；SAP—Tg基因型小鼠，模型构建成功。结论将ApoE^-/-小鼠与SAP—Tg小鼠杂交2代后成功建立了ApoE^-/-；SAP—Tg小鼠模型，并可通过筛选出的F2代ApoE^-/-小鼠和ApoE^-/-；SAP—Tg小鼠交配进行繁育筛选工作。
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| 关 键 词： | ApoE^- - 小鼠 血清淀粉样蛋白P 血清淀粉样蛋白P过表达（SAP—Tg）小鼠 动物模型 动脉粥样硬化 |
Establishment of the ApoE^-/- ;SAP-Tg mouse model |
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| Authors: | WU Teng XING Liying GOU Hongju LAN Tian ZHENG Lingyun GU Quliang WEN Dingwen WANG Lijing |
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| Institution: | 1. Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou 510006, China; 2. School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China ; 3. The Institute of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, China) |
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| Abstract: | Objective To establish the ApoE^-/-; SAP-Tg mouse model. Methods Female SAP-Tg mice were mated with male ApoE^-/- mice. The progeny of mice were genotyped by PCR. Results After female SAP-Tg mice were mated with male ApoE^-/- mice for three generations, ApoE^-/-; SAP-Tg mice were established. Conclusion ApoE^-/- ;SAP-Tg mice are successfully constructed through ApoE^-/- mice mating with SAP-Tg mice. |
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| Keywords: | ApoE gene deficient （ ApoE^-/- ） mice SAP SAP-Tg mice animal model atherosclerosis |
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