| 尾静脉注射STZ制备1型糖尿病大鼠模型实验的技巧 |
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| 引用本文： | 王继红,刘学政,杨雪佳.尾静脉注射STZ制备1型糖尿病大鼠模型实验的技巧[J].辽宁医学院学报,2010,31(1):7-9. |
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| 作者姓名： | 王继红 刘学政 杨雪佳 |
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| 作者单位： | 1. 辽宁中医药大学,辽宁,沈阳,110032
2. 辽宁医学院,辽宁,锦州121000 |
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| 摘 要： | 目的探讨STZ尾静脉不同给药方法和补造模时不同给药剂量致大鼠糖尿病成模状况的差异。方法本实验通过尾静脉不同的给药方法（非盐水导入组、盐水导入组）以建立大鼠糖尿病模型。以及未成模的大鼠给予不同剂量的STZ补造模（全量组、2／3全量组）。观察给药后大鼠成模情况、一般状况及检测各组成模后3d、4W、8W血糖值的变化。结果盐水导入方法可以大幅度降低STZ尾静脉注射造模时的死亡率（6．52％），并提高成模率（73．9％）。补造模时2／3剂量组死亡率降低（6．67％，P〈0．05）且成模后血糖各组间，各时间点无差异（P〉0．05）。结论盐水导入的尾静脉给药方法是注射链脲佐菌素致大鼠糖尿病模型的最佳途径，补造模时以2／3剂量为宜。
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| 关 键 词： | 链脲佐菌素 盐水导入法 糖尿病模型 大鼠 |
Empirical Skills of Establishing Type 1 Diabetie Model Rat by Injection Through Vena Caudalis Streptozotocin |
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| WANG Jihong,LIU Xuezheng,YANG Xuejia.Empirical Skills of Establishing Type 1 Diabetie Model Rat by Injection Through Vena Caudalis Streptozotocin[J].Journal of Liaoning Medical University (LNMU) Bimonthly,2010,31(1):7-9. |
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| Authors: | WANG Jihong LIU Xuezheng YANG Xuejia |
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| Institution: | 1.Liaoning University of Traditional Chinese Medicine/a>;Shenyang 110032 China/a>;2.Liaoning Medical University/a>;Jinzhou 121000 China |
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| Abstract: | Objective To explore the impact on induction of diabetes in rat models with different STZ administration and dosage.Methods SD rats received caudal vein injection of STZ to induce diabetes,with or without saline preceding the STZ injection.The impact of different additional amounts of STZ on the induction of diabetic model was also observed.The percentage of diabetic models,general condition and the blood glucose were determined on 3d,4w and 8w after STZ injection.Results The method of saline usage signific... |
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| Keywords: | streptozotocin importing with liquor natrii chloride diabetie model rats |
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