| 健脾清化方对2型糖尿病大鼠肝脏糖异生的影响 |
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| 引用本文： | 邱艳,谭凌婕,陈清光,龚凡,李俊燕,韩煦,柴菽彬,蔡梦洁,陆灏.健脾清化方对2型糖尿病大鼠肝脏糖异生的影响[J].上海中医药大学学报,2018,32(4):82-88. |
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| 作者姓名： | 邱艳 谭凌婕 陈清光 龚凡 李俊燕 韩煦 柴菽彬 蔡梦洁 陆灏 |
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| 作者单位： | 上海中医药大学附属曙光医院内分泌科 |
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| 基金项目： | 国家自然科学基金面上项目(81774235)；上海中医药大学研究生“创新能力培养“专项科研项目 |
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| 摘 要： | 目的:探讨健脾清化方对2型糖尿病大鼠肝脏糖异生的影响。方法:取Wistar大鼠,除正常组外,采用高脂喂养联合小剂量链脲左菌素(STZ)腹腔注射诱导2型糖尿病大鼠模型,成模后随机分为模型组、健脾清化方组。健脾清化方组予以含生药15 g·kg~(-1)·d~(-1)的混悬液灌胃,正常组和模型组用同体积双蒸水灌胃。连续灌胃4周后观察各组大鼠葡萄糖耐量和丙酮酸耐量,Western blot法测大鼠肝脏糖异生相关蛋白pFOXO1、FOXO1、PEPCK、G6Pase蛋白的表达,RT-PCR法测大鼠肝脏PEPCK、G6Pase的mRNA表达。结果:腹腔葡萄糖耐量和丙酮酸耐量试验结果显示,与正常组比较,模型组各时间点的血糖和曲线下面积(AUC)显著增加(P0.01);与模型组比较,健脾清化方能降低各时间点的血糖和AUC(P0.05)。肝糖异生相关蛋白及mRNA结果显示,与正常组比较,模型组大鼠肝脏pFOXO1/FOXO1蛋白表达的比值显著降低(P0.01),G6Pase、PEPCK蛋白及mRNA表达显著增加(P0.01);与模型组比较,健脾清化方组大鼠肝脏pFOXO1/FOXO1蛋白表达的比值显著增加(P0.01),G6Pase、PEPCK蛋白及mRNA表达降低(P0.05)。结论:2型糖尿病大鼠肝脏糖异生增加;健脾清化方可通过影响2型糖尿病大鼠肝脏糖异生相关酶的表达,减少其肝脏糖异生,从而降低血糖。
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| 关 键 词： | 健脾清化方 2型糖尿病 糖异生 |
Effects of Jianpi Qinghua Recipe on hepatic gluconeogenesis in rats with type 2 diabetes mellitus |
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| QIU Yan,TAN Lingjie,CHEN Qingguang,GONG Fan,LI Junyan,HAN Xu,CHAI Shubin,CAI Mengjie and LU Hao.Effects of Jianpi Qinghua Recipe on hepatic gluconeogenesis in rats with type 2 diabetes mellitus[J].Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai,2018,32(4):82-88. |
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| Authors: | QIU Yan TAN Lingjie CHEN Qingguang GONG Fan LI Junyan HAN Xu CHAI Shubin CAI Mengjie and LU Hao |
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| Institution: | Department of Endocrinology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China,Department of Endocrinology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China,Department of Endocrinology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China,Department of Endocrinology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China,Department of Endocrinology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China,Department of Endocrinology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China,Department of Endocrinology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China,Department of Endocrinology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China and Department of Endocrinology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China |
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| Abstract: | |
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| Keywords: | Jianpi Qinghua Recipe type 2 diabetes mellitus gluconeogenesis |
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