| 补肾生精法对老龄大鼠睾丸蛋白表达谱的影响 |
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| 引用本文： | 陈通文,曾金雄,王海鹰,黄锦燕.补肾生精法对老龄大鼠睾丸蛋白表达谱的影响[J].广州中医药大学学报,2008,25(5). |
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| 作者姓名： | 陈通文 曾金雄 王海鹰 黄锦燕 |
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| 作者单位： | 1. 广东省中医院珠海医院男科,广东珠海,519015
2. 福州东方医院,福建福州,350000 |
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| 摘 要： | 目的]观察补肾生精法对老龄大鼠睾丸蛋白表达谱的影响.方法]选用20只22月龄老龄雄性SD大鼠随机分为2组,每组10只,分别为空白对照组和补肾生精组;补肾生精组给药剂量为0.05 g·kg-1·d-1,连续灌胃给药60 d.取大鼠右侧睾丸,采用双向凝胶电泳法分析空白对照组和补肾生精组大鼠睾丸差异表达蛋白.结果]与空白对照组比较,补肾生精组大鼠睾丸中有7种蛋白表达发生了显著变化,其中4种蛋白表达上调,3种下调.对7种差异表达蛋白点胶内酶切后进行质谱分析,其中5种蛋白分别鉴定为磷脂酰乙醇胺结合蛋白(phosphatidylethanolamihe-binding protein,PEBP)、热休克蛋白8(heat shock 70 kDa protein 8,HSP7C)、磷酸丙糖异构酶(triose-phosphate isomerase,TPIS)、3-磷酸甘油醛脱氢酶(glyceraldehyde-3-phosphate dehydrogenase,GAPDH)、谷胱苷肽-s-基转移酶1(ghtathione S-transferase Mu 1,GSTM1-1).结论]补肾生精法延缓衰老的作用可能与其能调节老龄大鼠睾丸蛋白表达有关.
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| 关 键 词： | 补肾生精法/治疗应用 衰老/中药疗法 蛋白质组 疾病模型 动物 大鼠 |
Effect of Therapy of Tonifying Kidney and Replenishing Essence on Protein Expression Profile in Testes of Aged Rats |
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| CHEN Tongwen,ZENG Jinxiong,WANG Haiying,HUANG Jinyan.Effect of Therapy of Tonifying Kidney and Replenishing Essence on Protein Expression Profile in Testes of Aged Rats[J].Journal of Guangzhou University of Traditional Chinese Medicine,2008,25(5). |
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| Authors: | CHEN Tongwen ZENG Jinxiong WANG Haiying HUANG Jinyan |
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| Institution: | CHEN Tongwen1,ZENG Jinxiong2,WANG Haiying1,HUANG Jinyan1(1.Dept.of ,rology,Zhuhai Hospital Affiliated to Guangdong Provincial Hospital of TCM,Zhuhai 519015 Guangdong,China,2.Dongfang Hospital of Fuzhou,Fuzhou 350000 Fujian,China) |
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| Abstract: | |
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| Keywords: | THERAPY OF TONIFYING KIDNEY AND REPLENISHING ESSENCE/therapeutic use AGING/TCD therapy PROTEOME DISEASE MODELS ANIMAL RATS |
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