体外诱导人脐带血间充质干细胞为软骨细胞的初步研究

目的探讨胰岛素样生长因子-1(IGF-1)体外诱导人脐带血间充质干细胞(MSCs)向软骨细胞分化的可能性。方法由人脐静脉获得MSCs,纯化培养后用含100ng/ml IGF-1的培养基诱导,扫描电镜鉴定诱导后细胞。结果来源于脐血的单个核细胞经体外培养贴壁后出现形态学上可见间充质样的细胞,间充质样细胞为类似成纤维样的细胞形态。IGF-1诱导16天后,MSCs开始呈现软骨细胞特点。结论人脐带血MSCs可在体外经IGF-1诱导为软骨细胞。

IGF- 1 induced condrogenic differentiation of mesenchymal stem cells isolated from human umbilici cord blood in vitro

Objective To explore the feasibility of mesenchymal stem cells(MSCs) from human umbilical cord blood(HUCB) differentiating into chondrocytes in vitro.Methods MSCs isolated from HUCB with Percoll density gradient solution were culture-expanded because the cells attached and grew on dishes,then MSCs were induced with 100 ng/ml insulin-like growth factor Ⅰ(IGF Ⅰ).The differentiated cells were identified by using scanning electron microscope(SEM).Results The HUCB-derived mononuclear cells,when set in culture,gave rise to adherent cells,which exhibited either a fibroblast-like or mesenchymal-like phenotype.16 days after IGF Ⅰ medium treatment,MSCs in monolayer cultures exhibited a chondrocytic phenotype.Conclusion MSCs can be cultured and differentiated into chondrocytes induced by IGF Ⅰ in vitro.