海南黎汉族冠心病ACE基因多态性与危险因素研究

目的探讨海南黎汉族冠心病（CHD）血管紧张素转换酶（ACE）基因多态性与危险因素的关系。方法采用聚合酶链反应（PCR）方法，检测海南黎族CHD患者150例及正常对照组150例以及汉族CHD患者150例及汉族正常对照组150例的ACE基因D／I多态性．观察DD、DI、Ⅱ基因型频率，并对所有普通PCR定为DD型的样本进行插入特异性PCR检测．以减少误分型率。结果海南黎族冠心病组DD、DI、Ⅱ基因的频率分别是24．7％、32．7％、42．6％，D及Ⅰ等位基因频率分别为41．0％、59．0％。黎族正常对照组DD、DI、Ⅱ基因型频率分别为14．0O％、44．0％、42．0％，D及Ⅰ等位基因频率分别为36．0％、64．0％，两组之间的DD、DI、Ⅱ基因频率及D、Ⅰ等住基因频率均有显著性差异（P〈0．05）；海南汉族冠心病组DD、DI、Ⅱ基因的频率分别是23．3％、45．4％、31．3％；D及Ⅰ等位基因频率分别为46．0％、54．0％。正常对照组DD、DI、Ⅱ基因型频率分别为13．3％、42．0％、44．7％；D及Ⅰ等位基因频率分别为34-3％、65．7％，两组之间的DD、DI、Ⅱ基因频率及D、Ⅰ等位基因频率均有显著性差异（P＜0．05）。且经多元逐步回归分析显示，海南黎汉族冠心病TG水平升高，HDL—C降低。结论血管紧张素转换酶多态性与黎汉族冠心病有显著关联，是海南黎汉族冠心病的主要致病基因，甘油三酯（TG）升高，高密度脂蛋白（HDL—C）降低是海南黎汉冠心病的危险因子。

Investigation into the ACE gene polymorphisms and risk factors in coronary heart disease of Li and Han Nationality in Hainan

Objective To probe into the angiotensin converting enzyme(ACE)gene polymorphisms and the risk factors in coronary heart disease(CHD) in the Hainan Li and Han nationality.Methods The polymorphisms of ACE gene insertion/deletion(I/D) in 150 patients with CHD and 150 healthy people from Hainan Li and Han nationality were determined by aid of PCR.The genotype frequencies and allele frequencies of DD, DI and II were observed.Specific PCR detection was performed for patients who have been determined as DD by norm...