| 1株抗肝肿瘤新城疫病毒溶瘤株F基因的克隆和测序 |
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| 引用本文： | 杨建林,樊晓晖,唐深,梁莹.1株抗肝肿瘤新城疫病毒溶瘤株F基因的克隆和测序[J].中国热带医学,2009,9(3):418-420. |
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| 作者姓名： | 杨建林 樊晓晖 唐深 梁莹 |
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| 作者单位： | 1. 三峡大学分子生物学研究所,湖北,宜昌,443002
2. 广西医科大学微生物与免疲学教研室,广西,南宁,530021 |
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| 摘 要： | 目的对我们发现的1株高效抗肝肿瘤新城疫病毒溶瘤株的F基因进行扩增、序列测定和分析。方法RT—PCR扩增F基因片段，序列测定后与国际标准株弱毒株La Sota、B1及强毒株HER33、ITA／45、TEX／48、JS206WD、YG03的F基因比较研究，并对这株病毒F蛋白分子结构的疏水性、抗原表位及进化树分析。结果扩增出的F基因片段核苷酸长度为1656bp，单一的开放读码框编码552个氨基酸，其核苷酸序列与以上7种NDV的F基因同源性分别为：87．1％、87．5％、91．4％、91．7％、87．5％、90．7％、92.4％，氨基酸序列同源性为：90．2％、89．7％、94．9％、93．8％、90．8％、94．4％、95．5％。F基因裂解位点的氨基酸序列为112R—R—Q—K—R—F117，与所有强毒株在这一区域的序列（112R／K—R—Q—K／R—R—F117）相符，疏水性与抗原表位均与强毒株相似，进化树分析属于基因Ⅵ型。结论推测新发现的1株高效抗肝肿瘤新城疫病毒为NDV基因Ⅵ型强毒株。
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| 关 键 词： | 新城疫病毒溶瘤株 F基因 序列分析 |
Clonging and sequence analysis of fusion glycoprotein gene of newcastle disease virus a highly lyric anti-liver tumor strain |
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| YANG Jian-lin,FAN Xiao-hui,TANG Shen,et al..Clonging and sequence analysis of fusion glycoprotein gene of newcastle disease virus a highly lyric anti-liver tumor strain[J].China Tropical Medicine,2009,9(3):418-420. |
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| Authors: | YANG Jian-lin FAN Xiao-hui TANG Shen |
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| Institution: | 1.Institute of Molecular and Biology;Three Gorges University;Yichang 443002;Hubei;P.R.China;2 Department of Microbiology and Immunology;Guangxi Medical University;Nanning 530021;Guangxi;P.R.China |
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| Abstract: | Objective To amplyfy a strain of Newcastle disease virus (NDV) isolated from duck. Its fusion glycoprotein (F) gene was cloned and analyzed. Methods The hydrophobiity,antigenicity and phylogenetic tree of NDV D817 strainof fusiion protein molecular structure wa analyzed. Results Its F gene had a single operning reading fragment (ORF) lf 1 656 bp and its encoded 552 amino acids, Compared with the published avirulent ones (LaSota, BI) and vilulent strains (HER33, ITA/45, TEX/48, JS206WD, YG03) of NDV. The hom... |
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| Keywords: | Lytic NDV strain F gene Sequence analysis |
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