荧光定量PCR检测性传播病原体结果分析

目的了解深圳市女性性服务(FCSW)性传播感染(STIs)的流行现况。方法选取深圳市女性性服务，采集其宫颈和阴道分泌物，采用荧光定量PCR分别检测其中的CT、NG、HPV及HSV-2，评价其感染状况。结果391例FCSW中，STIs病原体总阳性检出率为53．20％(208／391)，其中，CT阳性检出率最高，为27．62％；余依次为HPV、NG和HSV-2，阳性检出率分别为26．34％，21．23％和2．81％；在STIs病原体的阳性中，感染2种以上STIs病原体83例，占39．90％(83／208)，其中，83例NG阳性中，CT的阳性栓出率为45．78％；108例CT阳性中，NG的阳性栓出率为35．18％。结论深圳市FCSW人群中，STIs流行率较高；加大该人群的STIs筛查及健康教育，是当前预防和控制STIs传播的重要策略。

Detection of sexually transmitted infections from sexual workers by using fluorescent quantitative PCR.

Objective To determine Chlamydia trachomatis (CT), Neisseria gonorrhoeae, (NG), herpes simplex virus type-2 (HSV-2) and human papilloma virus (HPV) in order to understand major STIs(sexually transmitted infections) prevalence among FCSW(Female Commercial Sex workers) in Shenzhen City. Methods Select 291 FCSW and collect cervical secretions and vaginal fluid. Use fluorescent quantitative PCR to test for CT, NG, HSV and HPV, then analyses corresponding infection status. Results Among 291 FCSWs, the overall infection rate for all STIs was 53.20% (208/309); the CT infection rate was the highest at 27.62%; followed by HPV, NG and HSV-2, with infection rates of 26.34%, 21.23% and 2.81% respectively. Among those infected with STIs, 83 (39.90%, 83/208) were co-infected with two or more STIs. Among the 83 cases positive for NG, the CT infection rate was 45.78%. Among the 108 cases positive for CT, the NG infection rate was 35.18%. Conclusion This study shows the STI infection rate among Shenzhen FCSWs to be relatively high. It is believed that strengthening STI screening and health education in this group is currently an important policy for preventing and controlling the spread of STIs.